LOCUS       sequence-181493-        9578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of humanized PgCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9578)
  TITLE     Zhang lab Cpf1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pY114 (pcDNA3.1-huPgCpf1)
FEATURES             Location/Qualifiers
     source          1..9578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(99..116)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    105..329
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      375..803
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(462..481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     672..693
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(812..832)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        856..1066
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        878..1074
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      925..1060
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     987..1006
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1141..1935
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1195..1214)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1805..1824
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2109..2230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2146..2165)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2200..2219
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2279..2295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2279..2295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2292..2314)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2303..2319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2327..2333,2334..2351,2352..2357))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2372..2393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2510..2527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2681..3269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2761..2780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3440..4231,4232..4300))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4063..4082
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4301..4405)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4480..4499)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4671..5050
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5051..5254
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5205..5225
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5299..5318
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5299..5317
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5359..5368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5371..5391
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9469..9516
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(9523..9578,1..25)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 cccatatgat gtccccgact atgcctaaga attctgcaga tatccagcac agtggcggcc
       61 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
      121 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
      181 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
      241 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
      301 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
      361 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      421 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      481 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      541 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
      601 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
      661 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
      721 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
      781 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
      841 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      901 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
      961 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     1021 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     1081 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     1141 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     1201 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     1261 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     1321 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     1381 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     1441 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     1501 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     1561 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     1621 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     1681 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     1741 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     1801 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     1861 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     1921 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     1981 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     2041 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     2101 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     2161 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     2221 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     2281 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2341 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     2401 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     2461 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     2521 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2581 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2641 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2701 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2761 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2821 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2881 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2941 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3001 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3061 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3121 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3181 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3241 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3301 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3361 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3421 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3481 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3541 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3601 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3661 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3721 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3781 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3841 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3901 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3961 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4021 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4081 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4141 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4201 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4261 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4321 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     4381 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     4441 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     4501 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     4561 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     4621 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     4681 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     4741 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     4801 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     4861 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     4921 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     4981 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     5041 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     5101 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     5161 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     5221 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
     5281 ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt
     5341 aaacttaagc ttaccggtgc caccatggcc ccaaagaaga agcggaaggt cggtatccac
     5401 ggagtcccag cagccgaaaa catcttcgac cagttcatcg gcaagtacag cctgagcaag
     5461 accctgagat tcgagctgaa gcccgtgggc aagaccgagg acttcctgaa gatcaacaag
     5521 gtgttcgaga aggaccagac catcgacgac agctacaacc aggccaagtt ctacttcgac
     5581 agcctgcacc agaagttcat cgacgccgcc ctggccagcg acaagacaag cgagctgagc
     5641 ttccagaact tcgccgacgt gctggaaaag cagaacaaga tcatcctgga caagaaacgc
     5701 gagatgggcg ccctgcggaa gcgggacaaa aatgccgtgg gcatcgaccg gctgcagaaa
     5761 gagatcaacg acgccgagga catcatccag aaagaaaaag agaagatcta taaggacgtg
     5821 cggaccctgt tcgacaacga ggccgagagc tggaaaacct actaccagga acgcgaggtg
     5881 gacggcaaga agatcacctt cagcaaggcc gacctgaagc agaagggcgc cgatttcctg
     5941 accgccgctg gcatcctgaa ggtgctgaag tacgagttcc ccgaagagaa agagaaagag
     6001 ttccaggcca agaaccagcc cagcctgttc gtggaagaaa aagaaaaccc cggccagaag
     6061 cggtacatct ttgacagctt cgacaagttc gccggctacc tgaccaagtt ccagcagacc
     6121 aagaagaacc tgtacgccgc cgacggcacc tctacagccg tggccacaag aatcgccgac
     6181 aacttcatca tcttccacca gaacacaaag gtgttccggg acaagtacaa gaacaaccac
     6241 accgacctgg gcttcgacga agagaatatc ttcgagatcg agcgctacaa gaactgcctg
     6301 ctgcagcggg aaatcgagca catcaagaac gagaactcct acaacaagat tatcggccgc
     6361 atcaacaaga aaatcaaaga gtacagggac cagaaggcca aggacaccaa gctgaccaag
     6421 agcgacttcc cattcttcaa gaacctggat aagcagatcc tgggggaggt ggaaaaagag
     6481 aagcagctga tcgagaaaac ccgggaaaag accgaagagg acgtgctgat tgagcggttc
     6541 aaagagttta tcgagaacaa cgaggaacgg ttcaccgccg ccaagaaact gatgaacgcc
     6601 ttctgcaacg gcgagttcga gagcgagtac gagggcatct acctgaagaa caaggccatc
     6661 aacaccatca gccggcggtg gttcgtgtcc gacagggact tcgaactgaa gctgccccag
     6721 cagaagtcta agaacaagag cgagaagaac gagcccaaag tgaagaagtt tatctctatc
     6781 gccgagatca agaatgctgt ggaagaactg gacggcgaca tcttcaaggc cgtgttctac
     6841 gataagaaga ttattgccca ggggggcagc aagctggaac agttcctcgt gatctggaag
     6901 tatgagttcg agtacctgtt ccgcgacatc gagagagaga acggcgagaa gctgctgggc
     6961 tacgactcct gtctgaagat cgccaagcag ctgggaatct tcccacagga aaaagaagcc
     7021 cgcgagaagg ccaccgccgt gattaagaac tacgccgatg ccggcctggg catcttccag
     7081 atgatgaagt acttcagcct ggacgacaag gaccggaaga acacccccgg acagctgagc
     7141 accaacttct acgccgagta cgacggctac tacaaggact tcgagttcat caagtactac
     7201 aacgagttcc ggaatttcat caccaagaag cccttcgacg aggacaagat caagctgaac
     7261 ttcgagaacg gggccctgct gaaaggctgg gacgagaaca aagaatacga cttcatgggc
     7321 gtgatcctga agaaagaggg ccggctgtat ctgggcatca tgcacaagaa ccaccggaag
     7381 ctgtttcaga gcatgggcaa cgccaagggc gacaacgcca acagatacca gaagatgatc
     7441 tacaagcaga tcgccgacgc cagcaaggat gtgcccagac tgctgctgac cagcaagaaa
     7501 gccatggaaa agttcaagcc cagccaggaa atcctgcgga tcaagaaaga aaagaccttc
     7561 aagcgcgaga gcaagaactt ctccctgcgg gacctgcacg ccctgatcga gtactaccgg
     7621 aactgcatcc cccagtacag caattggagc ttctacgact tccagtttca agacaccggg
     7681 aagtaccaga acatcaaaga gttcaccgac gacgtgcaga agtacggcta caagatcagc
     7741 tttcgggaca tcgacgatga gtacatcaat caggccctga acgagggcaa gatgtatctg
     7801 ttcgaggtcg tgaacaagga catctacaac accaagaacg gcagcaagaa tctgcacacc
     7861 ctgtactttg agcacatcct gagcgccgag aacctgaacg accccgtgtt caagctgagc
     7921 ggcatggccg agatcttcca gcggcagcct agcgtgaacg agagagaaaa gatcaccacc
     7981 cagaaaaacc agtgcattct ggacaagggc gatcgggcct acaagtaccg gcggtacacc
     8041 gagaagaaaa ttatgttcca catgagcctg gtgctgaaca ccggcaaggg cgagatcaaa
     8101 caggtgcagt tcaacaaaat catcaaccag cggatcagca gctccgacaa tgagatgaga
     8161 gtgaacgtga tcggcatcga cagaggcgag aaaaacctgc tgtactacag cgtcgtgaaa
     8221 cagaacgggg agatcatcga gcaggccagc ctgaatgaga tcaatggcgt gaactaccgc
     8281 gacaagctga ttgaacgcga gaaagagcgg ctgaagaacc ggcagtcctg gaagcctgtc
     8341 gtgaaaatca aggatctgaa gaagggctac atcagccacg tgatccacaa gatctgccag
     8401 ctgattgaga agtacagcgc catcgtggtg ctggaagatc tgaacatgcg gttcaagcag
     8461 attagaggcg gcatcgagcg gagcgtgtac cagcagtttg agaaggccct gattgacaag
     8521 ctgggctatc tggtgttcaa ggacaaccgg gacctgagag cccctggcgg cgtgctgaat
     8581 ggctatcagc tgagcgcccc cttcgtgtcc ttcgagaaga tgcggaagca gaccggcatc
     8641 ctgttctata cacaagccga gtacacctcc aagaccgacc ccatcaccgg ctttcggaag
     8701 aacgtgtaca tcagcaacag cgcctccctg gataagatca aagaagccgt gaagaaattc
     8761 gacgccatcg gctgggatgg caaagagcag agctacttct tcaagtacaa cccctacaac
     8821 ctggccgacg agaagtataa gaatagcacc gtgtccaaag agtgggccat cttcgccagc
     8881 gcccccagaa tcagacggca gaaaggcgag gatggctact ggaagtacga ccgcgtgaaa
     8941 gtgaacgagg aatttgagaa actgctgaaa gtgtggaact tcgtgaaccc caaggccaca
     9001 gacatcaagc aggaaatcat taagaaagag aaggccggcg acctgcaggg ggagaaagaa
     9061 ctggatggcc ggctgagaaa cttctggcac agctttatct acctgttcaa cctggtgctg
     9121 gaactgcgga acagcttcag cctgcagatc aagatcaagg ccggggaagt gatcgccgtg
     9181 gacgagggcg tggactttat cgccagcccc gtgaagcctt tcttcaccac ccccaatccc
     9241 tacatcccca gcaatctgtg ctggctggcc gtggaaaatg ccgatgccaa cggcgcctac
     9301 aatatcgccc ggaagggcgt gatgattctg aaaaagatca gagagcacgc caagaaggac
     9361 cctgagttca agaagctgcc taacctgttc atctccaacg ccgagtggga cgaggccgcc
     9421 agagactggg gcaaatacgc cggaaccaca gccctgaacc tggaccacaa gaggccggcg
     9481 gccacgaaga aggccggcca ggccaagaag aagaagggat cctacccata cgatgttcca
     9541 gattacgctt atccctacga cgtgcctgat tatgcata
//
