LOCUS       sequence-181495-        9377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of humanized Mb3Cpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9377)
  TITLE     Zhang lab Cpf1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pY117 (pcDNA3.1-huMb3Cpf1)
FEATURES             Location/Qualifiers
     source          1..9377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3408..3455
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3462..3542
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(3616..3633)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3622..3846
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3892..4320
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3979..3998)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4189..4210
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4329..4349)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4334..4663
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4514..4649
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4576..4595
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4730..5524
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4784..4803)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5394..5413
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5698..5819
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5735..5754)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5789..5808
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5868..5884)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5868..5884)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5881..5903)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5892..5908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5916..5922,5923..5940,5941..5946))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5961..5982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6099..6116)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6270..6858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6350..6369)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7029..7820,7821..7889))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7652..7671
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7890..7994)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8069..8088)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8260..8639
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8640..8843
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8794..8814
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8888..8907
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8888..8906
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      8948..8957
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8960..8980
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcgccaagct gttcaaggac ggcaaagagc tgggcgacct ggccaagttc gtgatcgccc
       61 aggaaggcga gagcagccct aagctggccc acctggccca tttcgagaag ttcagcacct
      121 acttcaccgg cttccacgac aaccgcaaga acatgtacag cgacgaggac aagcacaccg
      181 ccattgccta ccggctgatc cacgagaacc tgccccggtt catcgacaac ctgcagatcc
      241 tggccaccat caagcagaag cacagcgccc tgtacgacca gatcatcaac gagctgaccg
      301 ccagcggcct ggatgtgtct ctggcctctc acctggatgg ctaccacaag ctgctgacac
      361 aggaaggcat caccgcctac aacaccctgc tgggcggaat ctctggcgag gccggcagca
      421 gaaagatcca gggcatcaat gaactgatca acagccacca caaccagcac tgccacaaga
      481 gcgagcggat tgccaagctg cggcccctgc acaagcagat tctgagcgac ggaatgggcg
      541 tgtccttcct gcccagcaag ttcgccgacg acagcgaagt gtgccaggcc gtgaatgagt
      601 tctaccggca ctacgccgac gtgttcgcca aggtgcagag cctgttcgac ggcttcgacg
      661 actatcagaa agacggcatc tacgtggagt acaagaacct gaacgagctg tccaagcagg
      721 ccttcggcga cttcgctctg ctgggaagag tgctggacgg ctactatgtg gacgtcgtga
      781 accccgagtt caacgagaga tttgccaagg ccaagaccga caacgccaaa gccaagctga
      841 caaaagagaa ggacaagttc atcaagggcg tgcactccct ggcttctctg gaacaggcca
      901 tcgagcacta caccgccaga cacgacgacg agtctgtgca ggccggaaag ctgggccagt
      961 acttcaagca cggactggcc ggcgtggaca accccatcca gaagatccac aacaaccact
     1021 ctacaatcaa gggcttcctg gaaagagagc ggcctgccgg cgaaagagcc ctgcccaaga
     1081 tcaagagcga caagagcccc gagatcagac agctgaaaga gctgctggac aacgccctga
     1141 acgtggccca cttcgccaaa ctgctgacca ccaagaccac actgcacaat caggatggca
     1201 acttctacgg cgagttcgga gccctgtatg atgagctggc caagatcgcc accctgtaca
     1261 acaaagtgcg ggactacctg agccagaagc ccttcagcac cgagaagtac aagctgaatt
     1321 tcggcaaccc taccctgctg aacggctggg acctgaacaa agagaaagat aacttcggcg
     1381 tgatcctgca gaaggacggc tgctactacc tggccctgct ggataaggcc cacaagaagg
     1441 tgttcgataa cgcccccaac accggcaagt ctgtgtatca gaagatgatc tataagctgc
     1501 tgcctggccc caacaagatg ctgcccaagg tgttcttcgc caagagcaac ctggactact
     1561 acaaccccag cgccgaactg ctggacaaat acgcccaggg cacacacaag aaaggcgaca
     1621 acttcaacct gaaggactgc cacgccctga tcgatttctt caaggccgga atcaacaagc
     1681 accccgagtg gcagcacttc ggcttcaagt tcagccccac cagcagctac caggacctga
     1741 gcgacttcta cagagaggtg gaaccccagg gctaccaagt gaagttcgtg gacatcaacg
     1801 ccgactacat caatgagctg gtggaacagg gccagctgta cctgtttcag atctacaaca
     1861 aggactttag ccccaaggcc cacggcaagc ccaacctgca caccctgtat ttcaaggccc
     1921 tgtttagcga ggacaacctc gtgaatccca tctacaaact gaacggggag gccgagatct
     1981 tctaccggaa ggcctccctg gacatgaacg agacaaccat ccacagagcc ggcgaggtgc
     2041 tggaaaacaa gaaccctgac aaccctaaga aacggcagtt cgtgtacgac atcatcaagg
     2101 acaagcggta cacccaggat aagttcatgc tgcacgtgcc catcaccatg aacttcggag
     2161 tgcagggcat gaccatcaaa gagttcaaca aaaaagtgaa ccagagcatc cagcagtacg
     2221 acgaagtgaa cgtgatcggc atcgaccggg gcgagcggca tctgctgtat ctgaccgtga
     2281 tcaactccaa gggcgagatt ctggaacaga gatccctgaa cgacatcacc accgcctccg
     2341 ccaacggcac ccagatgacc accccttacc acaagatcct ggacaagcgc gagatcgagc
     2401 ggctgaacgc cagagtggga tggggagaga tcgagacaat caaagaactg aagtccggct
     2461 acctgtccca cgtggtgcat cagatctccc agctgatgct gaagtacaac gccatcgtgg
     2521 tgctggaaga tctgaatttt ggcttcaaga ggggccggtt caaggtggaa aagcagatct
     2581 accagaactt cgagaatgcc ctgatcaaga aactgaacca cctggtgctg aaagacaagg
     2641 ccgacgacga gatcggcagc tacaagaacg ccctgcagct gactaacaac ttcaccgatc
     2701 tgaagagtat cggcaagcag accggctttc tgttctacgt gcccgcctgg aataccagca
     2761 agatcgaccc cgagacaggc ttcgtggacc tgctgaagcc tagatacgag aatatcgccc
     2821 agagccaggc cttcttcggc aagttcgaca agatctgcta caacgccgat aggggctact
     2881 tcgagttcca catcgactac gccaagttca atgacaaggc caaaaacagc cggcagatct
     2941 ggaaaatctg cagccacggc gataagcgct acgtgtacga taagaccgcc aaccagaaca
     3001 agggcgccac catcggagtg aatgtgaacg atgagctgaa gtccctgttc acccgctacc
     3061 acatcaacga taagcagccc aatctcgtga tggacatctg ccagaacaac gacaaagagt
     3121 ttcacaagag cctgatgtat ctgctgaaaa cactgctggc tctgcggtac agcaacgcca
     3181 gctccgacga ggatttcatc ctgagccccg tggccaacga cgagggcgtg ttcttcaata
     3241 gcgccctggc cgacgatacc cagccccaga atgccgatgc caacggcgcc taccacattg
     3301 ccctgaaggg actgtggctg ctgaacgaac tgaagaacag cgacgatctg aacaaagtga
     3361 agctggccat cgacaaccag acctggctga atttcgccca gaacagaaag aggccggcgg
     3421 ccacgaagaa ggccggccag gccaagaaga agaagggatc ctacccatac gatgttccag
     3481 attacgctta tccctacgac gtgcctgatt atgcataccc atatgatgtc cccgactatg
     3541 cctaagaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt
     3601 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     3661 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     3721 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     3781 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     3841 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     3901 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3961 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4021 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4081 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4141 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4201 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4261 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4321 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4381 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4441 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4501 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4561 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     4621 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     4681 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4741 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4801 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     4861 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     4921 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     4981 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5041 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5101 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5161 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5221 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     5281 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5341 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5401 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5461 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5521 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5581 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5641 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     5701 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5761 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5821 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     5881 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     5941 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6001 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6061 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6121 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6181 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6241 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6301 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6361 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6421 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6481 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6541 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6601 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6661 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6721 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6781 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6841 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6901 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6961 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7021 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7081 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7141 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7201 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7261 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7321 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7381 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7441 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7501 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7561 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7621 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7681 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7741 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7801 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7861 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7921 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7981 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     8041 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8101 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     8161 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     8221 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     8281 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     8341 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     8401 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     8461 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     8521 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     8581 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     8641 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     8701 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     8761 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     8821 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
     8881 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct
     8941 taccggtgcc accatggccc caaagaagaa gcggaaggtc ggtatccacg gagtcccagc
     9001 agccctgttc caggacttca cccacctgta ccccctgagc aagaccgtca gattcgagct
     9061 gaagcccatc ggcaagaccc tggaacacat ccacgccaag aactttctga accaggacga
     9121 gacaatggcc gacatgtacc agaaagtgaa ggccatcctg gacgactacc accgggactt
     9181 tatcgccgac atgatgggcg aagtgaagct gaccaagctg gccgagttct acgacgtgta
     9241 cctgaagttc cggaagaacc ccaaggacga cggcctgcag aagcagctga aagacctgca
     9301 ggctgtgctg cggaaagaaa tcgtgaagcc tatcggaaac ggcggcaagt acaaggccgg
     9361 ctacgacaga ctgttcg
//
