LOCUS       sequence-181497-        9266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of humanized Lb4Cpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9266)
  TITLE     Zhang lab Cpf1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pY118 (pcDNA3.1-huLb4Cpf1)
FEATURES             Location/Qualifiers
     source          1..9266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..123)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    112..336
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      382..810
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(469..488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     679..700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(819..839)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        824..1153
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1004..1139
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1066..1085
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1220..2014
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1274..1293)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1884..1903
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2188..2309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2225..2244)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2279..2298
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2358..2374)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2358..2374)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2371..2393)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2382..2398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2406..2412,2413..2430,2431..2436))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2451..2472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2589..2606)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2760..3348)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2840..2859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3519..4310,4311..4379))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4142..4161
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4380..4484)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4559..4578)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4750..5129
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5130..5333
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5284..5304
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5378..5397
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5378..5396
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5438..5447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5450..5470
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9164..9211
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(9218..9266,1..32)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 atgcataccc atatgatgtc cccgactatg cctaagaatt ctgcagatat ccagcacagt
       61 ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
      121 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
      181 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
      241 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
      301 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
      361 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      421 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      481 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      541 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
      601 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
      661 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
      721 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
      781 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
      841 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
      901 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      961 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     1021 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     1081 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     1141 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     1201 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     1261 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     1321 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     1381 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     1441 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     1501 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     1561 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     1621 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     1681 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     1741 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     1801 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     1861 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     1921 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     1981 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     2041 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     2101 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     2161 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     2221 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2281 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     2341 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2401 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2461 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2521 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2581 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2641 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2701 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2761 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2821 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2881 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2941 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3001 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3061 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3121 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3181 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3241 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3301 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3361 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3421 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3481 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3541 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3601 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3661 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3721 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3781 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3841 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3901 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3961 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4021 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4081 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4141 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4201 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4261 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4321 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4381 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4441 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4501 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     4561 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     4621 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     4681 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     4741 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     4801 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     4861 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     4921 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     4981 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     5041 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     5101 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     5161 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     5221 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     5281 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
     5341 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
     5401 cccaagctgg ctagcgttta aacttaagct taccggtgcc accatggccc caaagaagaa
     5461 gcggaaggtc ggtatccacg gagtcccagc agccggcctg tacgacggct tcgtgaacag
     5521 atacagcgtg tccaagaccc tgagattcga gctgatcccc cagggccgga ccagagagta
     5581 catcgagaca aacggcatcc tgagcgacga cgaggaacgg gccaaggact acaagaccat
     5641 caagcggctg atcgacgagt accacaagga ttacatcagc cggtgcctga agaacgtgaa
     5701 catcagctgc ctggaagagt actaccacct gtacaacagc agcaaccggg acaagcggca
     5761 cgaggaactg gacgccctgt ccgaccagat gagaggcgag atcgccagct tcctgaccgg
     5821 caacgatgag tacaaagagc agaagtcccg cgacatcatc atcaacgagc ggatcatcaa
     5881 cttcgccagc accgacgaag aactggccgc cgtgaagcgg ttccggaagt tcaccagcta
     5941 cttcaccggc tttttcacca accgcgagaa catgtacagc gccgagaaga agtccaccgc
     6001 cattgcccac cggatcatcg acgtgaacct gcccaaatac gtggacaaca tcaaggcctt
     6061 caacaccgcc atcgaggccg gcgtgttcga tatcgccgag ttcgagagca acttcaaggc
     6121 catcaccgat gagcacgagg tgtccgacct gctggacatc accaagtaca gccggttcat
     6181 ccggaacgag gatatcatca tctacaacac cctgctgggc ggcatcagca tgaaggacga
     6241 gaagatccag ggcctgaatg agctgatcaa cctgcacaac cagaagcacc ccggcaagaa
     6301 ggtgcccctg ctgaaggtgc tgtacaagca gatcctgggc gacagccaga cccacagctt
     6361 cgtggacgac cagttcgagg acgatcagca agtgatcaat gccgtgaagg ccgtgaccga
     6421 caccttctcc gagacactgc tgggcagcct gaagatcatc attaacaaca tcggccacta
     6481 cgacctggac cggatctata tcaaggccgg ccaggatatc accaccctga gcaagagagc
     6541 cctgaacgac tggcacatca tcaccgagtg cctggaaagc gagtacgacg acaagttccc
     6601 caagaacaag aagtccgaca cctacgagga aatgcgcaac agatacgtga agtccttcaa
     6661 gagcttcagc atcggccggc tgaacagcct cgtgaccacc tataccgagc aggcctgttt
     6721 cctggaaaac tacctgggca gcttcggcgg cgacaccgac aagaattgcc tgaccgactt
     6781 caccaacagc ctgatggaag tggaacatct gctgaactcc gagtaccccg tgaccaatcg
     6841 gctgatcacc gactacgaga gcgtgcgcat cctgaagcgg ctgctggata gcgaaatgga
     6901 agtgatccac tttctgaagc ctctgctggg aaacggcaac gagagcgaca aggacctggt
     6961 gttctacggc gagtttgagg ccgagtacga gaagctgctg cccgtgatca aggtgtacaa
     7021 tagagtgcgg aactacctga cccggaagcc ctttagcaca gagaagatca agctgaactt
     7081 caacagccct accctgctgt gtggctggtc ccagagcaaa gaaaaagagt acatgggcgt
     7141 gatcctgcgg aaggacggcc agtactatct gggcatcatg acccccagca acaagaagat
     7201 ctttagcgag gcccccaagc ccgacgagga ctgctatgag aagatggtgc tgcggtacat
     7261 cccccacccc taccagatgc tgcccaaggt gttcttcagc aagagcaata tcgccttctt
     7321 caaccccagc gacgagatcc tgagaatcaa gaagcaggaa tcctttaaga agggcaagag
     7381 ctttaaccgg gacgactgcc acaagttcat cgacttctac aaggactcca tcaaccggca
     7441 cgaagagtgg cgcaagttca atttcaagtt cagcgatacc gatagctacg aggacatctc
     7501 ccggttctac aaagaggtgg aaaaccaggc cttcagcatg agcttcacca agatccccac
     7561 cgtgtacatc gacagcctgg tggacgaggg caagctgtac ctgtttaagc tgcacaacaa
     7621 ggatttcagc gagcacagca agggcaagcc caatctgcac acagtgtact ggaatgccct
     7681 gttcagcgag tacaacctgc agaataccgt gtaccagctg aacggctccg ccgagatctt
     7741 tttcagaaag gccagcatcc ccgagaacga gcgcgtgatc cacaagaaaa acgtgcccat
     7801 cacccggaag gtggccgagc tgaatggcaa gaaagaagtg tccgtgttcc cctacgacat
     7861 cattaagaac cggcggtaca ccgtggataa gttccagttt cacgtgcccc tgaagatgaa
     7921 tttcaaggcc gatgagaaga agcggatcaa cgacgacgtg atcgaggcca tcagaagcaa
     7981 caagggcatc cacgtgatcg gcatcgaccg gggcgagcgg aatctgctgt acctgagcct
     8041 gatcaacgag gaaggcagaa tcatcgagca gcggtccctg aacatcatcg actccggcga
     8101 gggccacacc cagaactaca gagatctgct ggattcccgc gagaaggaca gagagaaggc
     8161 ccgggaaaac tggcaggaaa ttcaggaaat caaggatctg aaaaccggct acctgtccca
     8221 ggccatccac accatcacaa agtggatgaa ggagtacaac gccatcatcg tgctggaaga
     8281 tctgaacgac agattcacca acggccggaa aaaagtggaa aaacaggtgt accagaaatt
     8341 cgagaagatg ctgatcgata agctgaatta ctatgtggac aaggacgaag agttcgaccg
     8401 gatgggcggc acccatagag ccctgcagct gaccgagaag ttcgagtcct tccagaagct
     8461 gggccggcag accggcttca tcttctacgt gcccgcctgg aacaccagca agctggaccc
     8521 taccaccgga ttcgtggacc tgctgtatcc caagtacaag agcgtggacg ccaccaagga
     8581 ctttatcaag aagtttgact tcatccggtt caacagcgag aagaactact tcgagttcgg
     8641 cctgcactac tccaacttca ccgagcgggc catcggctgc cgggacgagt ggattctgtg
     8701 cagctacggc aaccggatcg tgaacttcag aaacgccgcc aaaaacaaca gctgggatta
     8761 caaagagatc gacattacca aacagctgct ggacctgttt gagaagaacg gcatcgatgt
     8821 gaaacaggaa aacctgatcg actccatctg cgagatgaag gacaagccat tcttcaagtc
     8881 cctgatcgcc aacattaagc tgatcctgca gatccggaac agcgccagcg gcaccgacat
     8941 cgactacatg atcagccccg ccatgaacga tcggggcgag ttcttcgaca ccagaaaggg
     9001 cctgcagcag ctgcccctgg acgccgatgc taacggcgcc tacaacattg ccaagaaagg
     9061 cctgtggatc gtggaccaga tcagaaatac caccggcaac aacgtgaaga tggccatgag
     9121 caacagagaa tggatgcact ttgcccagga aagccggctg gccaagaggc cggcggccac
     9181 gaagaaggcc ggccaggcca agaagaagaa gggatcctac ccatacgatg ttccagatta
     9241 cgcttatccc tacgacgtgc ctgatt
//
