LOCUS       sequence-18150-e         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for cloning and expressing your gene on 
            a bicistronic mRNA also encoding IRES-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pCAGIG
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(23..31,32..34,35..609)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(196..215)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     425..442
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gctcctcgcc cttgctcacc atggttgtgg ccatattatc atcgtgtttt tcaaaggaaa
       61 accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta aacacatgta
      121 aagcatgtgc accgaggccc cagatcagat cccatacaat ggggtacctt ctgggcatcc
      181 ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac aactatccaa
      241 ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac acgtggcttt
      301 tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag ggtcgctaca
      361 gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac attcaacaga
      421 ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa gaagacaggg
      481 ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa taacatatag
      541 acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg ggggggggag
      601 ggagaggggg gggggggggg gnggaanttt nnntannngn cccnnnnccn cnaggnattc
      661 cttgncnnaa tgang
//
