LOCUS       sequence-181503-        9287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of humanized BfCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9287)
  TITLE     Zhang lab Cpf1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pY125 (pcDNA3.1-huBfCpf1)
FEATURES             Location/Qualifiers
     source          1..9287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(36..56)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        41..370
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      221..356
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     283..302
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             437..1231
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(491..510)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1101..1120
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1405..1526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1442..1461)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1496..1515
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1575..1591)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1575..1591)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1588..1610)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1599..1615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1623..1629,1630..1647,1648..1653))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1668..1689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1806..1823)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1977..2565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2057..2076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2736..3527,3528..3596))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3359..3378
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3597..3701)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3776..3795)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3967..4346
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4347..4550
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4501..4521
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4595..4614
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4595..4613
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4655..4664
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4667..4687
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8402..8449
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8456..8536
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(8610..8627)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8616..8840
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(8886..9287,1..27)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8973..8992)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9183..9204
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
       61 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      121 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      181 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
      241 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
      301 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
      361 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
      421 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
      481 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
      541 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
      601 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
      661 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
      721 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
      781 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
      841 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
      901 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
      961 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     1021 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     1081 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     1141 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     1201 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     1261 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     1321 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     1381 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     1441 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     1501 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     1561 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     1621 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     1681 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     1741 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     1801 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1861 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1921 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1981 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2041 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2101 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2161 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2221 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2281 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2341 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2401 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2461 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2521 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2581 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2641 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2701 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2761 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     2821 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     2881 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     2941 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     3001 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     3061 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     3121 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     3181 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     3241 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     3301 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     3361 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     3421 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     3481 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     3541 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     3601 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3661 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3721 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     3781 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     3841 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     3901 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     3961 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     4021 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     4081 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     4141 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     4201 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     4261 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     4321 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     4381 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     4441 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     4501 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
     4561 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
     4621 aagctggcta gcgtttaaac ttaagcttac cggtgccacc atggccccaa agaagaagcg
     4681 gaaggtcggt atccacggag tcccagcagc ctactacgag agcctgacca agctgtaccc
     4741 catcaagaaa accatccgga acgagctggt gcccatcggc aagaccctgg aaaacatcaa
     4801 gaagaacaac atcctggaag ccgacgagga ccggaagatc gcctacatca gagtgaaggc
     4861 catcatggac gactaccaca agcggctgat caacgaggcc ctgagcggct tcgccctgat
     4921 cgacctggat aaggccgcca acctgtacct gagcagaagc aagagcgccg acgacatcga
     4981 gagcttcagc cggttccagg acaagctgcg gaaggccatt gccaagagac tgagagagca
     5041 cgagaacttc ggcaagatcg gcaacaagga catcatcccc ctgctgcaga agctgagcga
     5101 gaacgaggac gattacaacg ccctggaatc cttcaagaac ttctacacct acttcgagag
     5161 ctacaacgac gtgcggctga acctgtacag cgacaaagaa aagtccagca ccgtggccta
     5221 ccgcctgatc aatgagaacc tgcccagatt cctggacaac atccgggcct acgacgccgt
     5281 gcagaaggcc ggcatcacaa gcgaggaact gagcagcgag gcccaggacg gcctgttcct
     5341 cgtgaacacc ttcaacaacg tgctgatcca ggatggcatc aacacctaca acgaggatat
     5401 cggcaagctg aacgtggcca tcaatctgta caaccagaaa aacgccagcg tgcagggctt
     5461 cagaaaggtg cccaagatga aggtgctgta caagcagatc ctgagcgacc gggaagagtc
     5521 cttcatcgac gagttcgaga gcgacaccga gctgctggac agcctggaaa gccactacgc
     5581 caatctggcc aagtacttcg gcagcaacaa ggtgcagctg ctgttcaccg ccctgcgcga
     5641 gtctaagggc gtgaacgtgt acgtgaagaa cgatatcgcc aagaccagct tcagcaacgt
     5701 ggtgttcggc agctggtccc ggatcgacga gctgattaac ggcgagtacg acgacaacaa
     5761 caatcggaag aaggacgaga agtactacga caagaggcag aaagagctga agaaaaacaa
     5821 gagctacacc atcgagaaga tcatcaccct gagcaccgag gacgtggacg tgatcggcaa
     5881 gtatatcgag aagctggaaa gcgacatcga cgatatccgg ttcaagggca agaatttcta
     5941 tgaggccgtg ctgtgcggcc acgaccgcag caagaagctg tccaagaaca agggcgccgt
     6001 ggaagccatc aagggctacc tggacagcgt gaaggacttc gagcgggacc tgaagctgat
     6061 caatggcagc ggccaggaac tggaaaagaa cctggtggtg tacggcgagc aggaagccgt
     6121 gctgagcgaa ctgagcggca tcgacagcct gtacaacatg acccggaact acctgacaaa
     6181 gaagcccttc agcaccgaga agattaagct gaacttcaac aagcccacct tcctggacgg
     6241 ctgggactac ggcaacgagg aagcctacct gggcttcttc atgatcaaag agggcaacta
     6301 ctttctggcc gtgatggacg ccaactggaa caaagagttc agaaacatcc ccagcgtgga
     6361 caagagcgac tgctacaaga aagtgatcta taagcagatc agcagccccg agaagtccat
     6421 ccagaatctg atggtcatcg atggcaagac cgtgaagaag aacggccgga aagagaaaga
     6481 aggcatccac agcggcgaga acctgattct ggaagaactg aagaacacct acctgcccaa
     6541 gaagatcaac gacatccgga agcggcggag ctacctgaac ggcgacacct tctccaagaa
     6601 ggacctgacc gagttcatcg gctactacaa acagcgcgtg atcgagtact acaacggcta
     6661 cagcttctac ttcaagtccg acgacgacta cgccagcttc aaagaatttc aggaagatgt
     6721 gggccggcag gcctaccaga tcagctatgt ggatgtgccc gtgtccttcg tggacgacct
     6781 gatcaacagc ggcaaactgt acctgttccg ggtgtacaac aaggatttca gcgagtacag
     6841 caagggcaga ctgaacctgc acaccctgta cttcaagatg ctgttcgacg agcgcaacct
     6901 gaagaatgtg gtgtacaagc tgaatggcca ggccgaggtg ttctaccggc ccagctctat
     6961 caagaaagag gaactgatcg tgcacagagc cggcgaggaa atcaagaaca aaaaccccaa
     7021 gagagccgcc cagaagccca ccagaaggct ggactacgac attgtgaagg accggcggta
     7081 cagccaggat aagttcatgc tgcacaccag catcatcatg aactttggcg ccgaggaaaa
     7141 tgtgtccttt aacgacatcg tgaacggcgt gctgcggaat gaggacaaag tgaatgtgat
     7201 cggaatcgac cggggcgagc ggaacctgct gtacgtggtc gtgattgacc ccgagggaaa
     7261 gatcctggaa cagcggagcc tgaactgcat caccgacagc aacctggaca tcgaaaccga
     7321 ttaccacaga ctgctggacg agaaagagag cgatagaaag atcgccagac gggactggac
     7381 cacaatcgag aacatcaaag aactgaaggc cggctacctg agccaggtgg tgcacattgt
     7441 ggccgaactg gtgctgaagt acaacgccat catctgcctg gaagatctga atttcggctt
     7501 caagcggggc agacagaaag tggaaaaaca ggtgtaccag aaatttgaga agatgctgat
     7561 tgacaaactg aattacctcg tgatggataa gagccgggaa cagctgtccc ctgagaagat
     7621 ctctggcgcc ctgaacgctc tgcagctgac ccccgacttc aagagcttca aggtgctggg
     7681 aaagcagacc ggcatcatct actacgtgcc cgcctacctg accagcaaga tcgaccccat
     7741 gaccggcttt gccaatctgt tctacgtgaa gtatgagaat gtggacaagg ccaaagagtt
     7801 tttcagcaag ttcgacagca tcaagtataa caaggacggc aagaactgga ataccaaggg
     7861 ctatttcgag ttcgccttcg actacaagaa gttcaccgat agagcctacg gccgggtgtc
     7921 cgagtggacc gtgtgtacag tgggcgagag aatcatcaag ttcaaaaaca aagagaagaa
     7981 caatagctac gatgacaaag tgatcgatct gaccaacagc ctgaaagaac tgttcgactc
     8041 ctacaaagtg acctacgagt ctgaggtgga cctgaaagac gctatcctgg ccatcgacga
     8101 ccccgccttc taccgcgatc tgacaagacg gctgcagcag accctgcaga tgcggaacag
     8161 ctcctgcgac ggcagccggg actacatcat cagccctgtg aagaactcca agggcgagtt
     8221 cttctgctcc gacaacaatg acgacaccac ccccaacgac gccgacgcca atggcgcctt
     8281 caatatcgcc agaaagggcc tgtgggtgct gaacgagatc agaaacagcg aggaaggctc
     8341 caagatcaac ctggccatga gcaacgccca gtggctggaa tacgcccagg acaacaccat
     8401 caagaggccg gcggccacga agaaggccgg ccaggccaag aagaagaagg gatcctaccc
     8461 atacgatgtt ccagattacg cttatcccta cgacgtgcct gattatgcat acccatatga
     8521 tgtccccgac tatgcctaag aattctgcag atatccagca cagtggcggc cgctcgagtc
     8581 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     8641 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     8701 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     8761 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     8821 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     8881 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     8941 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     9001 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     9061 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     9121 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     9181 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     9241 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagc
//
