LOCUS       sequence-181511-       10267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression for Cas13a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10267)
  AUTHORS   East-Seletsky A, O'Connell MR, Burstein D, Knott GJ, Doudna JA
  TITLE     RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas 
            Enzymes.
  JOURNAL   Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 
            10.1016/j.molcel.2017.04.008.
  PUBMED    28475872
REFERENCE   2  (bases 1 to 10267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.04"; journalName: "Mol Cell"; date: 
            "2017-05-4- 4"; volume: "66"; issue: "3"; pages: "373-383.e3"
COMMENT     Sequence Label: p2CT-His-MBP-Lba_Cas13a_WT
FEATURES             Location/Qualifiers
     source          1..10267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(233..238,239..255,256..261))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(288..306)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        374..478
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(479..547,548..1339)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(697..716)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1510..2098
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1999..2018
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2252..2269
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2284..2423
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2349..2368)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2509..2531
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2525..2716)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     4174..4193
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4277..4296
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4277..4295
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4384..4406
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4426..4443
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4453..5553
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     5527..5550
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             5608..5628
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(10073..10091)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      10087..10134
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg
       61 gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag
      121 cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat
      181 ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc
      241 ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg
      301 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      361 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      421 tccgctcatg agacaataac cctgataaat gcttcaataa cattgaaaaa ggaagagtat
      481 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      541 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      601 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      661 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      721 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      781 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      841 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      901 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      961 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1021 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1081 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1141 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1201 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1261 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1321 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1381 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1441 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1501 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1561 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1621 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1681 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1741 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1801 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1861 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1921 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1981 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2041 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2101 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2161 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2221 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaca
     2281 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc
     2341 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     2401 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     2461 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     2521 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
     2581 cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
     2641 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     2701 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
     2761 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     2821 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
     2881 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
     2941 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
     3001 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
     3061 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
     3121 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
     3181 gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg
     3241 cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat
     3301 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt
     3361 cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc
     3421 ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt
     3481 gacgatcagc ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag
     3541 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
     3601 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
     3661 cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc
     3721 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     3781 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     3841 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     3901 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     3961 tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag
     4021 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
     4081 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
     4141 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
     4201 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
     4261 ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctagt
     4321 gccggctccg gagagctctt taattaagcg gccgccctgc aggactcgag ttctagaaat
     4381 aattttgttt aactttaaga aggagatata catatgaaat cttctcacca tcaccatcac
     4441 catggttctt ctatgaaaat cgaagaaggt aaactggtaa tctggattaa cggcgataaa
     4501 ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc
     4561 accgttgagc atccggataa actggaagag aaattcccac aggttgcggc aactggcgat
     4621 ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg
     4681 ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat
     4741 gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg
     4801 atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg
     4861 gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac
     4921 ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag
     4981 tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg
     5041 gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct
     5101 gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc
     5161 gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc
     5221 aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg
     5281 gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa
     5341 gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca
     5401 cgtattgccg ccactatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag
     5461 atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag
     5521 actgtcgatg aagccctgaa agacgcgcag actaattcga gctcgaacaa caacaacaat
     5581 aacaataaca acaacctcgg gatcgaggaa aacctgtact tccaatccaa tgcaatgaag
     5641 atcagtaaag tacgcgaaga aaaccgtggg gccaaattga ctgtgaacgc caagactgct
     5701 gtagtgtccg agaaccgttc gcaagaaggg atcctgtata atgacccaag tcgttacggt
     5761 aaaagccgca aaaatgacga ggatcgtgac cgctacatcg agtcccgtct taagtcttct
     5821 gggaaattat accgcatttt taatgaggac aagaacaaac gcgagacaga cgaactgcaa
     5881 tggttcctta gtgagatcgt gaagaagatc aatcgtcgca atggtttggt attatcggat
     5941 atgcttagtg tggacgaccg cgcattcgag aaagccttcg aaaagtatgc agagttatcg
     6001 tatacgaatc gccgtaataa agtttctggt agcccggcgt tcgagacctg cggagtagat
     6061 gcagcaacag ctgaacgttt aaaaggcatt atctcggaaa ccaactttat caaccgtatc
     6121 aaaaacaata tcgacaacaa agtctcggag gacattatcg atcgtattat cgctaagtac
     6181 ttgaagaaaa gcttgtgtcg tgagcgtgtc aaacgtgggt taaagaaact tttaatgaat
     6241 gccttcgact tgccttacag tgaccctgac atcgatgtgc aacgcgattt tattgattac
     6301 gttttagagg atttctacca tgttcgtgcc aagtcacaag tgtctcgttc gatcaaaaac
     6361 atgaatatgc cggtgcagcc cgaaggcgat ggtaagtttg cgatcacagt aagtaagggt
     6421 gggacggaat cggggaacaa acgctctgcc gaaaaagaag cattcaaaaa gttcctgagt
     6481 gattacgcgt cgctggatga acgtgttcgt gacgacatgc ttcgtcgcat gcgccgcctg
     6541 gtcgtcttgt acttttacgg atcagacgat agcaaattat cagatgttaa cgaaaaattc
     6601 gacgtatggg aggaccatgc tgcccgccgc gtagacaatc gcgagttcat caagcttccg
     6661 ttggagaata aacttgccaa cggcaagacc gataaagatg cggaacgtat ccgtaagaac
     6721 accgttaaag aactgtatcg taaccaaaat attggatgct atcgccaggc tgttaaagcc
     6781 gtagaggagg acaacaacgg tcgctacttc gacgacaaga tgctgaacat gttctttatt
     6841 caccgcatcg agtacggtgt ggaaaagatt tacgccaatt tgaaacaagt cacggagttc
     6901 aaggcccgca ctgggtatct ttcagagaaa atctggaaag acttgatcaa ttacatttcc
     6961 attaaataca tcgctatggg caaggcagtc tacaattacg ccatggatga gctgaatgcc
     7021 tcagacaaaa aggagatcga gctgggcaag atcagtgaag aatatttgtc cgggatcagt
     7081 tcgtttgact atgaattgat caaagccgaa gagatgctgc aacgcgagac cgctgtatat
     7141 gttgctttcg cggctcgcca cttgtcctcc cagaccgttg aactggattc cgagaactcc
     7201 gacttccttc ttttgaagcc aaagggaact atggacaaga acgataaaaa caagttagcc
     7261 tccaacaaca ttcttaattt tcttaaggac aaggaaacac ttcgcgatac cattttacag
     7321 tactttggcg gccacagtct gtggaccgac tttccgtttg acaaatactt ggccggcggg
     7381 aaagacgacg tcgacttcct gacagacctt aaagatgtga tttactccat gcgtaatgat
     7441 agttttcact atgctactga aaaccacaac aatggaaaat ggaataagga attaattagt
     7501 gcaatgtttg aacatgaaac agagcgtatg acagtagtca tgaaagataa attctactcg
     7561 aataatcttc ctatgttcta taagaatgac gacttgaaga aattgctgat cgatttgtac
     7621 aaggataacg tagagcgtgc gagccaggtc ccatctttta acaaggtctt cgtgcgcaag
     7681 aattttcctg ctttggttcg cgataaagac aacttgggga ttgaattaga ccttaaggcg
     7741 gacgcggata aaggtgagaa cgagttaaaa ttctacaacg ctctttatta catgtttaaa
     7801 gagatctact ataacgcgtt cttaaatgat aagaatgtgc gtgaacgttt tattactaag
     7861 gcgacgaaag tggccgataa ctatgatcgc aataaggagc gcaaccttaa agaccgtatt
     7921 aagtccgcgg ggtcggatga gaaaaaaaag ttacgtgaac agctgcagaa ttacattgct
     7981 gagaatgact tcgggcagcg tatcaagaat atcgtgcagg tcaatccgga ttataccctg
     8041 gctcaaatct gccaacttat tatgacggag tataatcaac aaaacaacgg gtgcatgcag
     8101 aaaaagtctg ctgcccgcaa agacatcaat aaggatagtt accagcatta caagatgctg
     8161 cttttggtta atttacgcaa agcatttttg gaatttatta aggagaatta tgcttttgtt
     8221 ttgaaaccct ataaacacga cctttgtgat aaagccgatt tcgtgccaga tttcgccaaa
     8281 tacgtcaaac cctacgctgg cttgatcagc cgtgtagcag gatcaagtga gctgcaaaag
     8341 tggtacatcg tgagccgctt cttgtctccg gcacaggcca atcacatgct tgggttcctg
     8401 cactcgtaca agcagtacgt atgggatatt taccgccgcg catccgaaac cggtacagag
     8461 attaaccact ctatcgcaga ggacaagatt gctggggttg acattaccga cgtcgacgca
     8521 gtgatcgatt tatcggtgaa attgtgcgga acaatctcta gcgagatttc ggattatttc
     8581 aaggatgacg aggtctacgc ggagtacatc tcatcatatt tagatttcga atacgatggt
     8641 ggtaattaca aagactcgct gaaccgtttc tgtaattcag acgcagtaaa tgaccaaaaa
     8701 gtggcactgt attatgacgg cgaacacccg aaattgaatc gtaacattat cttgtcgaaa
     8761 ctttacggag agcgccgttt cttggagaaa atcactgacc gcgtttctcg ttcagatatt
     8821 gtggagtatt acaagctgaa gaaggagacg tcgcagtatc agacgaaagg gattttcgat
     8881 agtgaggacg aacagaagaa tattaagaag tttcaggaga tgaaaaacat cgtggaattt
     8941 cgtgatctga tggattactc ggagatcgcg gacgaattac aaggacaact gatcaactgg
     9001 atttatcttc gtgaacgtga cctgatgaac ttccaactgg ggtatcatta cgcctgcttg
     9061 aacaacgact caaataagca agctacttat gtaactcttg attaccaagg taaaaaaaac
     9121 cgcaagatta acggtgcgat tttatatcag atttgtgcaa tgtatattaa tggactgccg
     9181 ctttactacg tcgataaaga cagttcggag tggacggtga gcgacggtaa ggaatccacg
     9241 ggagccaaga ttggtgaatt ctaccgctat gcaaagtcct ttgaaaatac gtctgactgc
     9301 tacgcttcgg gcttagagat cttcgagaat atcagtgagc acgacaacat cacggaatta
     9361 cgcaactaca ttgaacattt tcgttactac agctcttttg accgttcctt tttggggatc
     9421 tattcagagg tgtttgatcg ctttttcaca tacgacttaa agtatcgcaa aaacgtacct
     9481 accatcttgt ataatatttt actgcaacac tttgttaatg tccgcttcga gttcgtaagc
     9541 ggcaaaaaaa tgattggcat tgacaaaaag gaccgcaaga ttgccaaaga gaaggaatgc
     9601 gctcgtatta caatccgcga aaagaacggt gtctactcgg agcagttcac ttacaagttg
     9661 aagaatggga ccgtgtatgt cgatgcgcgt gacaaacgct acctgcagtc cattattcgt
     9721 cttttgtttt accccgaaaa ggtcaacatg gacgaaatga tcgaagttaa ggaaaagaag
     9781 aagccgagtg acaataatac gggaaagggc tatagcaaac gcgaccgcca gcaggatcgt
     9841 aaagaatatg acaagtacaa agaaaaaaag aaaaaggagg ggaatttcct ttcaggtatg
     9901 ggaggcaata tcaactggga cgagattaac gctcaactta agaactagta aattggaagt
     9961 ggataacgga tccgcgatcg tggcgcgcca cctggtggcc ggccggtacc acgcgtgcgc
    10021 gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
    10081 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
    10141 ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat
    10201 agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc
    10261 tccgaga
//
