LOCUS       sequence-181515-        9989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression for Cas13a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   East-Seletsky A, O'Connell MR, Burstein D, Knott GJ, Doudna JA
  TITLE     RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas 
            Enzymes.
  JOURNAL   Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 
            10.1016/j.molcel.2017.04.008.
  PUBMED    28475872
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.04"; journalName: "Mol Cell"; date: 
            "2017-05-4- 4"; volume: "66"; issue: "3"; pages: "373-383.e3"
COMMENT     Sequence Label: p2CT-His-MBP-Ere_Cas13a_WT
FEATURES             Location/Qualifiers
     source          1..9989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      81..669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     570..589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     823..840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    855..994
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(920..939)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1080..1102
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1096..1287)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2745..2764
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2848..2867
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2848..2866
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2955..2977
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2997..3014
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3024..4124
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     4098..4121
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             4179..4199
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(8366..8384)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8380..8427
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(8793..8798,8799..8815,8816..8821))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8848..8866)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8934..9038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9039..9107,9108..9899)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9257..9276)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
       61 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
      121 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      181 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
      241 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      301 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
      481 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
      661 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      721 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      781 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      841 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
      901 cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
      961 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     1021 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     1081 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
     1141 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc
     1201 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
     1261 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
     1321 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     1381 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga
     1441 ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc
     1501 acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga
     1561 cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca
     1621 ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt
     1681 ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat
     1741 catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga
     1801 gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg
     1861 caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct
     1921 tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca
     1981 aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca
     2041 taaatcgccg tgacgatcag cggtccaatg atcgaagtta ggctggtaag agccgcgagc
     2101 gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac
     2161 gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct
     2221 cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg
     2281 gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg
     2341 tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg
     2401 tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag
     2461 aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact
     2521 gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg
     2581 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
     2641 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
     2701 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
     2761 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
     2821 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt
     2881 ttccctctag tgccggctcc ggagagctct ttaattaagc ggccgccctg caggactcga
     2941 gttctagaaa taattttgtt taactttaag aaggagatat acatatgaaa tcttctcacc
     3001 atcaccatca ccatggttct tctatgaaaa tcgaagaagg taaactggta atctggatta
     3061 acggcgataa aggctataac ggtctcgctg aagtcggtaa gaaattcgag aaagataccg
     3121 gaattaaagt caccgttgag catccggata aactggaaga gaaattccca caggttgcgg
     3181 caactggcga tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc
     3241 aatctggcct gttggctgaa atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt
     3301 ttacctggga tgccgtacgt tacaacggca agctgattgc ttacccgatc gctgttgaag
     3361 cgttatcgct gatttataac aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga
     3421 tcccggcgct ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc
     3481 aagaaccgta cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg
     3541 aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc
     3601 tgaccttcct ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca
     3661 tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat
     3721 ggtccaacat cgacaccagc aaagtgaatt atggtgtaac ggtactgccg accttcaagg
     3781 gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc gccagtccga
     3841 acaaagagct ggcaaaagag ttcctcgaaa actatctgct gactgatgaa ggtctggaag
     3901 cggttaataa agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg
     3961 cgaaagatcc acgtattgcc gccactatgg aaaacgccca gaaaggtgaa atcatgccga
     4021 acatcccgca gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca
     4081 gcggtcgtca gactgtcgat gaagccctga aagacgcgca gactaattcg agctcgaaca
     4141 acaacaacaa taacaataac aacaacctcg ggatcgagga aaacctgtac ttccaatcca
     4201 atgcaatgct gcgtcgtgat aaggaagtga aaaagcttta taacgttttt aatcagatcc
     4261 aggtcggtac gaagcctaaa aaatggaata atgatgaaaa attgtcgccc gaagagaatg
     4321 aacgtcgcgc tcaacagaaa aatatcaaga tgaaaaacta caaatggcgt gaagcctgct
     4381 caaagtatgt tgagtcctcg caacgcatta tcaacgatgt tattttttac tcttatcgta
     4441 aagcgaaaaa taagttacgc tacatgcgca agaatgagga tatcttgaag aagatgcaag
     4501 aggcagagaa gttgtccaag ttcagtggcg gcaagttgga ggattttgtc gcatacacct
     4561 tgcgtaagtc gttagtagtc tccaagtacg atacacaaga gttcgatagt ttagctgcta
     4621 tggttgtatt ccttgagtgt attggtaaaa ataacatctc cgatcatgag cgcgagatcg
     4681 tttgtaaact gcttgaatta attcgtaagg actttagtaa gttggatcca aatgttaagg
     4741 ggtcacaagg tgcaaacatt gtccgcagtg tccgcaatca gaatatgatt gtacaacccc
     4801 agggggatcg tttccttttc ccccaggtgt atgcgaagga gaatgagacc gtcactaata
     4861 aaaatgtaga aaaagaaggt ttaaacgagt tccttctgaa ttacgcgaat cttgacgacg
     4921 agaaacgcgc ggaaagtttg cgcaaattgc gccgtattct tgacgtttac ttttctgcac
     4981 caaaccacta tgaaaaggac atggatatca cattaagcga caacattgaa aaggagaagt
     5041 tcaacgtgtg ggagaaacac gaatgtggca aaaaggagac aggcttattt gtggacatcc
     5101 cagatgtatt aatggaagca gaagcagaga acattaaatt agacgccgtg gtagagaaac
     5161 gtgagcgtaa agtcttaaac gatcgtgtac gtaaacaaaa catcatctgc taccgctata
     5221 cgcgcgcagt ggtggagaaa tacaattcca acgagccttt gttctttgag aataatgcca
     5281 tcaaccaata ctggatccac catattgaaa acgccgtcga acgtattctt aaaaactgta
     5341 aagcaggtaa gttgttcaag ctgcgcaagg ggtatcttgc agagaaagtc tggaaagatg
     5401 ccatcaactt aatctccatc aaatacattg cgttagggaa ggccgtatac aattttgcgt
     5461 tagacgacat ctggaaggac aagaaaaata aggaactggg gatcgtggat gagcgcatcc
     5521 gtaacggcat cactagtttt gattatgaaa tgatcaaggc gcacgagaac ttgcaacgtg
     5581 aacttgctgt ggacattgcg ttttcggtga acaaccttgc acgcgctgtt tgtgacatga
     5641 gtaacttggg taataaagaa tcggatttcc tgttgtggaa acgcaacgac attgccgaca
     5701 agttaaagaa caaggatgac atggctagcg ttagcgctgt actgcagttt ttcggtggca
     5761 agtccagctg ggatatcaac atttttaaag atgcctataa aggtaaaaag aaatacaatt
     5821 acgaagttcg ttttatcgac gatctgcgca aggcgatcta ttgtgcacgc aacgagaatt
     5881 tccatttcaa gacagcgttg gttaacgatg aaaagtggaa tactgaattg tttggcaaaa
     5941 tcttcgaacg cgagacggag ttttgcctta atgtggagaa agaccgcttc tactcgaaca
     6001 acttatacat gttctaccag gtaagtgaac tgcgcaacat gcttgatcac ctgtatagtc
     6061 gctcagtaag tcgcgccgcc caagtgcctt cttacaattc tgtaattgtt cgcactgctt
     6121 tccccgagta tatcacgaat gtattaggat atcagaaacc aagctacgat gctgacacgt
     6181 tagggaagtg gtacagcgcc tgctactatt tgttgaaaga aatctattac aattccttct
     6241 tacagtcgga tcgtgcgttg cagctttttg agaaatcagt taaaaccctg tcatgggacg
     6301 ataaaaaaca acaacgtgcg gtagataatt ttaaggatca cttttccgac attaaatcag
     6361 cctgcactag tttagcccag gtgtgtcaga tttatatgac ggagtacaac cagcagaaca
     6421 accaaattaa aaaggttcgc tcatcaaatg actctatttt cgatcaacca gtctaccaac
     6481 attacaaggt cttattgaag aaggcgattg caaacgcttt tgcggactat ttgaagaata
     6541 acaaggactt attcggtttt atcggcaaac cgttcaaggc taatgaaatc cgtgagattg
     6601 acaaagaaca gtttctgccc gactggacta gtcgcaaata cgaagcatta tgtatcgagg
     6661 ttagcggaag ccaagaattg cagaaatggt acatcgtagg taaattcttg aacgcccgta
     6721 gtttaaactt gatggtgggt tccatgcgct cctatatcca atacgtcaca gatattaaac
     6781 gccgtgcagc atctattggt aatgaattgc atgtatcggt acatgatgta gaaaaggtag
     6841 agaagtgggt gcaggtcatc gaggtttgtt ccttactggc ctcgcgtact agtaatcagt
     6901 tcgaagacta ctttaacgat aaggatgact acgcacgtta cttaaagtca tacgtcgatt
     6961 tttcaaacgt tgacatgccc tccgagtact cagccttagt ggattttagc aatgaggagc
     7021 aatctgattt gtatgtggac ccaaagaacc ctaaggtaaa tcgcaacatc gtgcactcga
     7081 aactgtttgc agcagatcat attttacgcg acatcgtcga gccagtcagc aaggataata
     7141 ttgaagaatt ttactcacag aaggcggaga ttgcttattg taaaatcaag gggaaagaga
     7201 tcacggcgga ggagcaaaaa gccgtgttga aatatcaaaa actgaaaaac cgtgtggaac
     7261 tgcgtgatat cgtagagtat ggcgaaatta tcaacgagtt gttgggacag ctgattaatt
     7321 ggagcttcat gcgtgaacgt gacttgttat acttccaact tggatttcat tacgactgtt
     7381 tacgcaacga tagtaagaaa cctgaaggtt ataagaatat taaagtggac gagaattcta
     7441 tcaaggacgc tatcttatac cagatcattg gaatgtatgt caatggggta actgtatacg
     7501 cgcctgaaaa agatggggat aaattgaagg aacagtgtgt gaaaggagga gtgggagtga
     7561 aagtaagtgc gttccaccgc tattctaaat atttaggact gaatgaaaaa acgctttaca
     7621 acgccggtct ggagattttc gaagtcgtag cggagcatga ggacattatc aacttacgca
     7681 acggcatcga tcactttaag tactatttgg gcgattatcg ttctatgttg tcgatttaca
     7741 gcgaagtctt cgatcgcttt ttcacttatg acattaaata ccagaaaaat gtattgaatt
     7801 tactgcaaaa tatccttttg cgccacaatg tcatcgtcga gccgattttg gagtcgggtt
     7861 tcaagaccat tggggagcag actaaaccag gtgcaaagtt gtcgattcgc agtattaaaa
     7921 gtgacacatt ccaatacaag gttaaaggcg ggacattgat tacagacgct aaggacgagc
     7981 gttaccttga aacgattcgt aagattctgt actacgctga aaatgaagag gataacctga
     8041 aaaagtcagt ggtcgttaca aacgctgaca aatacgagaa aaataaggag tcagacgacc
     8101 agaataaaca gaaagagaaa aagaataagg acaataaggg gaaaaagaac gaggagacaa
     8161 aatctgacgc cgagaaaaac aataacgaac gcttatcata caaccccttt gcgaatctta
     8221 actttaagct gagcaactaa taacattgga agtggataac ggatccgcga tcgcggcgcg
     8281 ccacctggtg gccggccggt accacgcgtg cgcgctgatc cggctgctaa caaagcccga
     8341 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     8401 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag
     8461 gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag
     8521 gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg
     8581 catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac
     8641 gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag
     8701 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact
     8761 gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca
     8821 aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     8881 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8941 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     9001 cctgataaat gcttcaataa cattgaaaaa ggaagagtat gagtattcaa catttccgtg
     9061 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     9121 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9181 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     9241 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     9301 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9361 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9421 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9481 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9541 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     9601 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9661 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9721 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9781 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9841 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9901 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9961 aaaggatcta ggtgaagatc ctttttgat
//
