LOCUS       sequence-181517-       10112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression for Cas13a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   East-Seletsky A, O'Connell MR, Burstein D, Knott GJ, Doudna JA
  TITLE     RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas 
            Enzymes.
  JOURNAL   Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 
            10.1016/j.molcel.2017.04.008.
  PUBMED    28475872
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.04"; journalName: "Mol Cell"; date: 
            "2017-05-4- 4"; volume: "66"; issue: "3"; pages: "373-383.e3"
COMMENT     Sequence Label: p2CT-His-MBP-Cam_Cas13a_WT
FEATURES             Location/Qualifiers
     source          1..10112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     103..122
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     356..373
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    388..527
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(453..472)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     613..635
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(629..820)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2278..2297
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2381..2400
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2381..2399
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2488..2510
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2530..2547
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2557..3657
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     3631..3654
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             3712..3732
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(8022..8040)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8036..8083
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(8449..8454,8455..8471,8472..8477))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8504..8522)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8590..8694
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8695..8763,8764..9555)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8913..8932)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9726..10112,1..202)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
       61 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      121 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      181 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      241 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      301 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      361 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
      421 cggtatttca caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
      481 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
      541 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
      601 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
      661 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct
      721 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
      781 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
      841 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
      901 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
      961 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag
     1021 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg
     1081 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc
     1141 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta
     1201 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg
     1261 acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg
     1321 tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat
     1381 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
     1441 ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc
     1501 ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct
     1561 cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca atgatcgaag ttaggctggt
     1621 aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag
     1681 catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa
     1741 ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat
     1801 gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc
     1861 ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct
     1921 ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag
     1981 ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg
     2041 gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga
     2101 ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag
     2161 gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
     2221 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
     2281 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
     2341 gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact cactataggg
     2401 agaccacaac ggtttccctc tagtgccggc tccggagagc tctttaatta agcggccgcc
     2461 ctgcaggact cgagttctag aaataatttt gtttaacttt aagaaggaga tatacatatg
     2521 aaatcttctc accatcacca tcaccatggt tcttctatga aaatcgaaga aggtaaactg
     2581 gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg taagaaattc
     2641 gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga agagaaattc
     2701 ccacaggttg cggcaactgg cgatggccct gacattatct tctgggcaca cgaccgcttt
     2761 ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc gttccaggac
     2821 aagctgtatc cgtttacctg ggatgccgta cgttacaacg gcaagctgat tgcttacccg
     2881 atcgctgttg aagcgttatc gctgatttat aacaaagatc tgctgccgaa cccgccaaaa
     2941 acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg
     3001 atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga cgggggttat
     3061 gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga taacgctggc
     3121 gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat gaatgcagac
     3181 accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat gaccatcaac
     3241 ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt aacggtactg
     3301 ccgaccttca agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc aggtattaac
     3361 gccgccagtc cgaacaaaga gctggcaaaa gagttcctcg aaaactatct gctgactgat
     3421 gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct gaagtcttac
     3481 gaggaagagt tggcgaaaga tccacgtatt gccgccacta tggaaaacgc ccagaaaggt
     3541 gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg tactgcggtg
     3601 atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc gcagactaat
     3661 tcgagctcga acaacaacaa caataacaat aacaacaacc tcgggatcga ggaaaacctg
     3721 tacttccaat ccaatgcaat gaagttcagt aaagtcgatc atacacgtag tgctgttggt
     3781 attcaaaagg ccacagatag cgttcacggg atgctgtata ctgaccccaa aaaacaggaa
     3841 gtcaatgatc ttgataaacg cttcgaccaa ttaaacgtaa aggcgaaacg cttgtataac
     3901 gtgttcaatc aatccaaagc ggaggaggac gatgacgaga aacgtttcgg aaaggtggta
     3961 aaaaaattaa atcgcgaact gaaggatctg ctttttcacc gtgaagtgtc tcgttataac
     4021 tccattggga acgccaagta caattactat gggattaaat ctaacccaga agaaattgtc
     4081 tccaacctgg gaatggtgga gagtcttaag ggcgagcgtg atccccaaaa ggttatctca
     4141 aaattactgc tttattacct tcgtaaggga cttaagccgg gaaccgacgg cctgcgtatg
     4201 attctggagg cttcatgtgg tttacgtaaa ctttccggtg acgaaaagga attaaaggtt
     4261 tttcttcaaa ccctggatga ggatttcgaa aagaagacgt ttaaaaaaaa cctgattcgc
     4321 tcgatcgaaa atcaaaatat ggccgttcag ccttccaatg agggtgatcc tatcatcggc
     4381 attacccagg gacgttttaa ctcgcagaag aacgaagaga aaagcgccat cgaacgcatg
     4441 atgtcaatgt atgctgacct gaatgaggat caccgtgaag atgtattacg caaactgcgt
     4501 cgccttaatg tcctgtattt taacgttgac accgaaaaaa cagaggagcc gacacttcca
     4561 ggcgaggtgg atacaaatcc cgtttttgag gtgtggcatg accacgaaaa aggcaaagaa
     4621 aatgaccgcc agtttgctac ctttgcaaaa attttaactg aagaccgtga gacacgtaaa
     4681 aaggagaagt tggctgtcaa agaggcgttg aacgatttga aatccgccat tcgcgaccat
     4741 aatattatgg cctaccgttg ctctattaag gttactgaac aggacaaaga cgggctgttc
     4801 ttcgaggatc aacgtatcaa tcgcttttgg atccaccaca tcgagagcgc agtcgagcgc
     4861 atcttagcat cgattaatcc cgaaaaattg tataaactgc gcattgggta tcttggagag
     4921 aaggtatgga aggatctttt gaactatctt tctattaaat acatcgccgt gggcaaggca
     4981 gtgtttcact tcgctatgga ggatttggga aaaactgggc aagacatcga attgggcaaa
     5041 ctgtcaaaca gtgtaagcgg cggcttaaca tccttcgatt acgagcagat tcgcgctgac
     5101 gaaacgcttc aacgtcagtt aagcgttgaa gttgcctttg cagcaaataa cctgttccgt
     5161 gccgttgtag gacagaccgg caagaagatt gagcaatcca aatctgagga gaatgaggag
     5221 gacttccttt tatggaaagc tgaaaaaatt gcggaatcaa ttaaaaaaga aggtgaggga
     5281 aacacgctta aaagtatctt gcaattcttc gggggagcga gctcctggga tttgaaccat
     5341 ttttgcgcag cgtatggtaa cgagtcgtct gcattagggt atgaaactaa attcgcagac
     5401 gacctgcgca aggcgattta ctcactgcgt aatgagacct ttcattttac tacacttaac
     5461 aaaggatcat ttgactggaa tgcaaagtta atcggtgaca tgtttagcca cgaggctgca
     5521 acggggatcg cagtagaacg cacgcgcttt tactcgaaca atttaccgat gttttaccgc
     5581 gagtcggact tgaaacgcat catggaccat ttatacaata cctaccaccc ccgtgcgtcc
     5641 caagttccta gttttaactc agttttcgtg cgcaagaatt tccgcctgtt tctgagtaat
     5701 acgctgaaca ccaatacctc attcgacact gaagtatacc aaaagtggga gagcggtgtc
     5761 tattatctgt tcaaggagat ctattataac agtttcttac caagtggtga cgcccaccat
     5821 cttttctttg aagggttacg tcgcatccgt aaagaagctg acaatctgcc gatcgtgggt
     5881 aaagaagcta agaaacgcaa tgcggttcaa gattttggtc gtcgttgtga cgaacttaag
     5941 aatctttctc tgagcgccat ttgccagatg attatgaccg aatataatga gcaaaataat
     6001 ggaaatcgta aggtcaagtc tacacgtgaa gataaacgta agccagatat tttccagcac
     6061 tataaaatgc tgttactgcg caccttgcag gaggcttttg cgatctatat tcgtcgcgaa
     6121 gagttcaagt ttatcttcga cttgccaaaa accctttatg tgatgaaacc agtcgaagag
     6181 ttcttgccta attggaaatc cgggatgttc gatagtttag tggaacgcgt aaagcagtcg
     6241 cccgacttac agcgctggta cgtgttgtgt aaatttttaa atgggcgttt gttgaatcaa
     6301 ttatcagggg taattcgctc atatatccag tttgcaggag atatccaacg ccgcgcaaaa
     6361 gctaatcata accgcttgta tatggataac acgcagcgcg ttgagtatta ttcaaacgtc
     6421 ttagaagtgg tggatttctg tattaagggc acttctcgct tctccaacgt gttctctgat
     6481 tatttccgcg acgaagatgc atatgccgat taccttgaca attacttgca atttaaagat
     6541 gagaaaatcg cggaggtctc cagttttgca gccttgaaga cattttgtaa cgaagaggag
     6601 gttaaagcag gaatttacat ggatggagaa aaccccgtta tgcagcgcaa tatcgttatg
     6661 gccaaacttt tcgggccgga tgaggttctg aaaaatgtgg ttccgaaggt tacgcgtgag
     6721 gagatcgagg aatattacca gttagagaaa cagattgctc catatcgcca gaatggatat
     6781 tgtaaatccg aggaggacca aaagaaactt cttcgctttc agcgtatcaa aaatcgtgtt
     6841 gaattccaga cgatcaccga gttcagcgag attattaatg agctgttagg acagctgatt
     6901 tcctggagct tcttacgtga acgcgacttg ctgtactttc agctgggctt tcactacctt
     6961 tgtcttcaca atgacacgga gaagcctgcc gaatacaagg aaatttctcg cgaggacggc
     7021 acagttatcc gtaatgccat tttgcatcag gtggctgcta tgtatgtggg tggattgccc
     7081 gtttacacct tagccgacaa gaagttggcc gcattcgaga agggcgaggc ggattgcaaa
     7141 ctgagcattt cgaaagatac agcaggggcc ggtaagaaga ttaaggattt tttccgctac
     7201 agtaaatacg tattgattaa agatcgtatg ctgaccgacc agaatcagaa atacacaatc
     7261 tacttagcgg gtctggagtt gttcgagaac acggatgaac atgacaatat tacggacgta
     7321 cgcaagtacg tagatcattt taagtattat gctacgagtg atgaaaacgc catgtctatt
     7381 cttgatctgt acagtgagat tcatgatcgt ttcttcacgt atgacatgaa gtatcaaaag
     7441 aacgtagcca acatgcttga gaatatcctt ttacgtcact tcgtgcttat ccgtcctgag
     7501 tttttcactg gtagtaagaa agtcggggaa ggaaaaaaga ttacatgtaa ggctcgtgca
     7561 caaattgaga ttgcagaaaa cgggatgcgt tcagaggact ttacgtataa actgtccgat
     7621 gggaaaaaga acattagcac ctgtatgatt gcggcacgcg accagaagta tctgaatacg
     7681 gtcgcgcgtt tactgtacta tccacacgag gcaaagaagt cgatcgtcga cacgcgcgaa
     7741 aagaaaaaca ataagaagac aaaccgcggt gacggcacct tcaataagca gaaaggtact
     7801 gcacgtaagg aaaaggataa tgggccccgc gaattcaacg atacgggctt tagcaatacg
     7861 ccatttgcag gtttcgaccc ctttcgtaat agctaataac attggaagtg gataacggat
     7921 ccgcgatcgc ggcgcgccac ctggtggccg gccggtacca cgcgtgcgcg ctgatccggc
     7981 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     8041 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     8101 atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa
     8161 gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg
     8221 gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga
     8281 tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta
     8341 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca
     8401 tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat
     8461 cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta
     8521 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     8581 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8641 ctcatgagac aataaccctg ataaatgctt caataacatt gaaaaaggaa gagtatgagt
     8701 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8761 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8821 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8881 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     8941 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     9001 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     9061 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     9121 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     9181 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     9241 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     9301 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     9361 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9421 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9481 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9541 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9601 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9661 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9721 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9781 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9841 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     9901 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9961 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10021 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10081 acgacctaca ccgaactgag atacctacag cg
//
