LOCUS       sequence-181527-        4830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Dang L, Yoon K, Wang M, Gaiano N
  TITLE     Notch3 signaling promotes radial glial/progenitor character in the 
            mammalian telencephalon.
  JOURNAL   Dev Neurosci. 2006;28(1-2):58-69.
  PUBMED    16508304
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev 
            Neurosci."; date: "2006"; volume: "28(1-2)"; pages: "58-69"
COMMENT     Sequence Label: pCAGGS-NICD
FEATURES             Location/Qualifiers
     source          1..4830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     336..358
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     424..443
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             484..507
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3370..3389)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3435..3490
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3489..3508)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3851..3867)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3851..3867)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3864..3886)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3875..3891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3899..3905,3906..3923,3924..3929))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3944..3965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4024..4219
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4070..4205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4225..4359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4262..4281)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4316..4335
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4427..4444)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4678..4697)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
       61 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
      121 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
      181 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
      241 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
      301 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
      361 cggctctagc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
      421 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg gaattcgacc
      481 atggactaca aagacgatga cgataaaggg gggatcattc cttacaagat tgaggccgtg
      541 aagagtgagc cggtggagcc tccgctgccc tcgcagctgc acctcatgta cgtggcagcg
      601 gccgccttcg tgctcctgtt ctttgtgggc tgtggggtgc tgctgtcccg caagcgccgg
      661 cggcagcatg gccagctctg gttccctgag ggtttcaaag tgtcagaggc cagcaagaag
      721 aagcggagag agcccctcgg cgaggactca gtcggcctca agcccctgaa gaatgcctca
      781 gatggtgctc tgatggacga caatcagaac gagtggggag acgaagacct ggagaccaag
      841 aagttccggt ttgaggagcc agtagttctc cctgacctga gtgatcagac tgaccacagg
      901 cagtggaccc agcagcacct ggacgctgct gacctgcgca tgtctgccat ggccccaaca
      961 ccgcctcagg gggaggtgga tgctgactgc atggatgtca atgttcgagg accagatggc
     1021 ttcacacccc tcatgattgc ctcctgcagt ggagggggcc ttgagacagg caacagtgaa
     1081 gaagaagaag atgcacctgc tgtcatctct gacttcatct accagggcgc cagcttgcac
     1141 aaccagacag accgcaccgg ggagaccgcc ttgcacttgg ctgcccgata ctctcgttca
     1201 gatgctgcaa agcgcttgct ggaggccagt gcagatgcca acatccagga caacatgggc
     1261 cgtactccgt tacatgcagc agtttctgca gatgctcagg gtgtcttcca gatcctgctc
     1321 cggaacaggg ccacagatct ggatgcccga atgcatgatg gcacaactcc actgatcctg
     1381 gctgcgcgcc tggccgtgga gggcatgctg gaggacctca tcaactcaca tgctgacgtc
     1441 aatgccgtgg atgacctagg caagtcggct ttgcattggg cggccgcggt gaacaatgtg
     1501 gatgctgctg ttgtgctcct gaagaacgga gccaacaagg acatgcagaa caacaaggag
     1561 gagactcccc tgttcctggc cgcccgtgag ggcagctatg agactgccaa agtgttgctg
     1621 gaccactttg ccaaccggga catcacggat cacatggacc gattgccgcg ggacatcgca
     1681 caggagcgta tgcaccacga tatcgtgcgg cttttggatg agtacaacct ggtgcgcagc
     1741 ccacagctgc atggcactgc cctgggtggc acacccactc tgtctcccac actctgctcg
     1801 cccaatggct acctgggcaa tctcaagtct gccacacagg gcaagaaggc ccgcaagccc
     1861 agcaccaaag ggctggcttg tggtagcaag gaagctaagg acctcaaggc acggaggaag
     1921 aagtcccagg atggcaaggg ctgcctgttg gacagctcga gcatgctgtc gcctgtggac
     1981 tccctcgagt caccccatgg ctacttgtca gatgtggcct cgccacccct cctcccctcc
     2041 ccattccagc agtctccatc catgcctctc agccacctgc ctggtatgcc tgacactcac
     2101 ctgggcatca gccacttgaa tgtggcagcc aagcctgaga tggcagcact ggctggaggt
     2161 agccggttgg cctttgagcc acccccgcca cgcctctccc acctgcctgt agcctccagt
     2221 gccagcacag tgctgagtac caatggcacg ggggctatga atttcaccgt gggtgcaccg
     2281 gcaagcttga atggccagtg taagtggctt ccccggctcc agaatggcat ggtgcccagc
     2341 cagtacaacc cactacggcc gggtgtgacg ccgggcacac tgagcacaca ggcagctggc
     2401 ctccagcata gcatgatggg gccactacac agcagcctct ccaccaatac cttgtccccg
     2461 attatttacc agggcctgcc caacacacgg ctggcaacac agcctcacct ggtgcagacc
     2521 cagcaggtgc agccacagaa cttacagctc cagcctcaga acctgcagcc accatcacag
     2581 ccacacctca gtgtgagctc ggcagccaat gggcacctgg gccggagctt cttgagtggg
     2641 gagcccagtc aggcagatgt acaaccgctg ggccccagca gtctgcctgt gcacaccatt
     2701 ctgccccagg aaagccaggc cctgcccaca tcactgccat cctccatggt cccacccatg
     2761 accactaccc agttcctgac ccctccttcc cagcacagtt actcctcctc ccctgtggac
     2821 aacaccccca gccaccagct gcaggtgcca gagcacccct tcctcacccc atcccctgag
     2881 tcccctgacc agtggtccag ctcctccccg cattccaaca tctctgattg gtccgagggc
     2941 atctccagcc cgcccaccac catgccgtcc cagatcaccc acattccaga ggcatttaaa
     3001 taaacagaga tgtgggatgc aggaccccag cttccgttcc caagccctgt tgggagtcct
     3061 ttccagtgct tcaggatgct ggggcgacca aaggagcctt ttaaaaaatg tttttataca
     3121 aaataagagg acaagaattt ccattttttt ttttagtatt tatttatgta cttttatttt
     3181 ccacagaaac actgcctttt tatttatatg tattgttttc tatggcacta gggaaaaaca
     3241 tatctgttcc aagaaaataa actagagata tcgcggccgc ctcgagcaat tcactcctca
     3301 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     3361 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     3421 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     3481 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     3541 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
     3601 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
     3661 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
     3721 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
     3781 cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg
     3841 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     3901 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     3961 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     4021 tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4081 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4141 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4201 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa
     4261 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4321 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc
     4381 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     4441 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4501 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4561 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4621 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4681 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4741 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4801 gctgtaggta tctcagttcg gtgtaggtcg
//
