LOCUS       sequence-181535-       11825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11825)
  TITLE     Zanpieri
REFERENCE   2  (bases 1 to 11825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p75 NTR
FEATURES             Location/Qualifiers
     source          1..11825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(21..40)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(700..1491,1492..1560))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1323..1342
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1561..1665)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1740..1759)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1931..2310
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2312..2510
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2465..2485
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2528..2708
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2755..2880
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3373..3606
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3791..3835
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4133..4250
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4315..5526
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5363..5382
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5590..5634
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             5635..5661
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(6865..7439,7440..7442,7443..7451)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7032..7049)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7259..7278
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7452..7454,7455..7457,7458..8171)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7497..7518)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7758..7777)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8105..8126
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8212..8800
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8265..8285)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8683..8694)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8803..8819)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8804..8820)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9325..9505
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9531..9548)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9537..9761
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9807..10235
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9894..9913)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10104..10125
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10244..10264)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10249..10578
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10429..10564
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10491..10510
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10626..10673
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10692..11066
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11196..11317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11233..11252)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11287..11306
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11366..11382)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11366..11382)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11379..11401)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11390..11406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11414..11420,11421..11438,11439..11444))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11459..11480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11597..11614)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
       61 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      121 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      181 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      241 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      301 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      361 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      421 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      481 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      541 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      601 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      661 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      721 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      781 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      841 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      901 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
      961 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1021 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1081 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1141 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1201 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1261 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1321 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1381 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1441 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1501 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1561 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1621 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1681 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     1741 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     1801 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     1861 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     1921 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     1981 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2041 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2101 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2161 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2221 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2281 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2341 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2401 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2461 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
     2521 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2581 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2641 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     2701 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     2761 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     2821 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     2881 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     2941 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3001 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3061 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3121 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3181 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     3241 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     3301 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     3361 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     3421 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     3481 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     3541 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     3601 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     3661 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     3721 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     3781 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     3841 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     3901 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     3961 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     4021 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     4081 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     4141 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     4201 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     4261 acagggacag cagagatcca gtttggttaa ttaacccgtg tcggctccag atctggcctc
     4321 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt
     4381 cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc
     4441 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
     4501 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
     4561 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
     4621 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
     4681 tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg
     4741 ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg
     4801 ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa
     4861 aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt
     4921 tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa
     4981 tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg
     5041 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc
     5101 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg
     5161 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
     5221 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
     5281 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
     5341 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
     5401 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
     5461 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
     5521 ttagacgaag cttgggctgc aggtcgactc tagaggatcc cggcgcgcct gttcgaacat
     5581 gctagcacca tgtctgcact tctgatccta gctcttgttg gagctgcagt tgcttatcca
     5641 tatgatgttc cagattatgc taaggagaca tgttccacag gcctgtacac ccacagcgga
     5701 gagtgctgca aagcctgcaa cttgggcgaa ggcgtggccc agccctgcgg agccaaccag
     5761 accgtgtgtg aaccctgcct ggacaatgtt acattctccg atgtggtgag cgccactgag
     5821 ccgtgcaagc cgtgcaccga gtgcctgggc ctgcagagca tgtccgctcc ctgtgtggag
     5881 gcagacgatg cagtgtgcag atgtgcctat ggctactacc aggacgagga gactggccac
     5941 tgtgaggctt gcagcgtgtg cgaggtgggc tcgggactcg tgttctcctg ccaggacaaa
     6001 cagaacacag tgtgtgaaga gtgcccagag ggcacatact cagacgaagc caaccacgtg
     6061 gacccgtgcc taccctgcac ggtgtgcgag gacactgagc gccagttacg cgagtgcacg
     6121 ccctgggctg atgctgaatg cgaagagatc cctggtcgat ggatcccaag gtctacgccc
     6181 ccggagggct ccgacagcac agcgcccagc acccaggagc ctgaggttcc tccagagcaa
     6241 gaccttgtac ccagtacagt ggcggatatg gtgaccactg tgatgggcag ctcccagcct
     6301 gtagtgaccc gcggcaccac cgacaacctc attcctgtct attgctccat cttggctgct
     6361 gtggtcgtgg gccttgtggc ctatattgct ttcaagaggt ggaacagctg caaacaaaat
     6421 aaacaaggcg ccaacagccg ccccgtgaac cagacgcccc caccggaggg agagaaactg
     6481 cacagcgaca gtggcatctc tgtggacagc cagagcctgc acgaccagca gacccatacg
     6541 cagactgcct caggccaggc cctcaagggt gatggcaacc tctacagtag cctgcccctg
     6601 accaagcgtg aggaggtaga gaaactgctc aacggggata cctggcgaca tctggcaggc
     6661 gagctgggtt accagcctga acatatagac tcctttaccc acgaggcctg cccagtgcga
     6721 gccctgctgg ccagctgggg tgcccaggac agtgcaacgc ttgatgccct tttagccgcc
     6781 ctgcgacgca tccagagagc tgacattgtg gagagtctat gcagcgagtc cactgccaca
     6841 tccccagtgt gaaccggtga tccgcccctc tccctccccc ccccctaacg ttactggccg
     6901 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     6961 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     7021 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     7081 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     7141 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     7201 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     7261 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     7321 gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa
     7381 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     7441 tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     7501 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     7561 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
     7621 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
     7681 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     7741 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     7801 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     7861 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     7921 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     7981 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     8041 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     8101 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     8161 tgtacaagta aagcggccgc gactctagaa ttcgatatca agcttatcga taatcaacct
     8221 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8281 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8341 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8401 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     8461 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     8521 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     8581 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     8641 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     8701 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     8761 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     8821 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     8881 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     8941 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     9001 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     9061 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     9121 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     9181 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     9241 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     9301 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     9361 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9421 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     9481 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     9541 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     9601 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     9661 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     9721 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     9781 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     9841 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     9901 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     9961 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    10021 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    10081 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    10141 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    10201 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
    10261 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    10321 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10381 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
    10441 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    10501 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    10561 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
    10621 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
    10681 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
    10741 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
    10801 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
    10861 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
    10921 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
    10981 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
    11041 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
    11101 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
    11161 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
    11221 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    11281 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
    11341 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    11401 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    11461 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    11521 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    11581 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    11641 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    11701 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    11761 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    11821 agagg
//
