LOCUS       sequence-181542-       10239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human VEGFR1 with Y to F mutation at position 1333.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Raikwar NS, Liu KZ, Thomas CP
  TITLE     N-terminal cleavage and release of the ectodomain of Flt1 is 
            mediated via ADAM10 and ADAM 17 and regulated by VEGFR2 and the Flt1
            intracellular domain.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112794. doi: 
            10.1371/journal.pone.0112794. eCollection 2014.
  PUBMED    25387128
REFERENCE   2  (bases 1 to 10239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112794. doi"; pages: " 
            10.1371/journal.pone.0112794. eCollection 2014"
COMMENT     Sequence Label: hFlt1 Y1333F
FEATURES             Location/Qualifiers
     source          1..10239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..18
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        179..558
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        559..762
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     713..733
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     788..807
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        788..806
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             955..981
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6262..6291
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6310..6333
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(6353..6369)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6353..6369)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6380..7002
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6579..6598)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      complement(7151..7739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7231..7250)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    8068..8115
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     8140..8159
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(8347..9141)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(8347..9141)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8458..8477)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9068..9087
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      9190..9324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        9321..9512
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     9469..9489
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(9514..9618)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9645..10100
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9732..9751)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9942..9963
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(10226..10239,1..9)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgtaaaacga cggccagtgc caagctgatc tatacattga atcaatattg gcaattagcc
       61 atattagtca ttggttatat agcataaatc aatattggct attggccatt gcatacgttg
      121 tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttga
      181 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      241 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      301 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      361 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      421 gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      481 cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta
      541 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg
      601 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      661 caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg
      721 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
      781 aattttgtaa tacgactcac tatagggcgg ccgggaattc gtcgactgga tccggtaccg
      841 aggagatctg ccgccgcgat cgccatggtc agctactggg acaccggggt cctgctgtgc
      901 gcgctgctca gctgtctgct tctcacagga tctagttcag gttcaaaatt aaaataccca
      961 tatgacgtcc cagactacgc tgatcctgaa ctgagtttaa aaggcaccca gcacatcatg
     1021 caagcaggcc agacactgca tctccaatgc aggggggaag cagcccataa atggtctttg
     1081 cctgaaatgg tgagtaagga aagcgaaagg ctgagcataa ctaaatctgc ctgtggaaga
     1141 aatggcaaac aattctgcag tactttaacc ttgaacacag ctcaagcaaa ccacactggc
     1201 ttctacagct gcaaatatct agctgtacct acttcaaaga agaaggaaac agaatctgca
     1261 atctatatat ttattagtga tacaggtaga cctttcgtag agatgtacag tgaaatcccc
     1321 gaaattatac acatgactga aggaagggag ctcgtcattc cctgccgggt tacgtcacct
     1381 aacatcactg ttactttaaa aaagtttcca cttgacactt tgatccctga tggaaaacgc
     1441 ataatctggg acagtagaaa gggcttcatc atatcaaatg caacgtacaa agaaataggg
     1501 cttctgacct gtgaagcaac agtcaatggg catttgtata agacaaacta tctcacacat
     1561 cgacaaacca atacaatcat agatgtccaa ataagcacac cacgcccagt caaattactt
     1621 agaggccata ctcttgtcct caattgtact gctaccactc ccttgaacac gagagttcaa
     1681 atgacctgga gttaccctga tgaaaaaaat aagagagctt ccgtaaggcg acgaattgac
     1741 caaagcaatt cccatgccaa catattctac agtgttctta ctattgacaa aatgcagaac
     1801 aaagacaaag gactttatac ttgtcgtgta aggagtggac catcattcaa atctgttaac
     1861 acctcagtgc atatatatga taaagcattc atcactgtga aacatcgaaa acagcaggtg
     1921 cttgaaaccg tagctggcaa gcggtcttac cggctctcta tgaaagtgaa ggcatttccc
     1981 tcgccggaag ttgtatggtt aaaagatggg ttacctgcga ctgagaaatc tgctcgctat
     2041 ttgactcgtg gctactcgtt aattatcaag gacgtaactg aagaggatgc agggaattat
     2101 acaatcttgc tgagcataaa acagtcaaat gtgtttaaaa acctcactgc cactctaatt
     2161 gtcaatgtga aaccccagat ttacgaaaag gccgtgtcat cgtttccaga cccggctctc
     2221 tacccactgg gcagcagaca aatcctgact tgtaccgcat atggtatccc tcaacctaca
     2281 atcaagtggt tctggcaccc ctgtaaccat aatcattccg aagcaaggtg tgacttttgt
     2341 tccaataatg aagagtcctt tatcctggat gctgacagca acatgggaaa cagaattgag
     2401 agcatcactc agcgcatggc aataatagaa ggaaagaata agatggctag caccttggtt
     2461 gtggctgact ctagaatttc tggaatctac atttgcatag cttccaataa agttgggact
     2521 gtgggaagaa acataagctt ttatatcaca gatgtgccaa atgggtttca tgttaacttg
     2581 gaaaaaatgc cgacggaagg agaggacctg aaactgtctt gcacagttaa caagttctta
     2641 tacagagacg ttacttggat tttactgcgg acagttaata acagaacaat gcactacagt
     2701 attagcaagc aaaaaatggc catcactaag gagcactcca tcactcttaa tcttaccatc
     2761 atgaatgttt ccctgcaaga ttcaggcacc tatgcctgca gagccctgag agatcccctc
     2821 ataatttccc caaaacgtaa ccatgtgtga atagattttg agtaagcagg gttgcagcca
     2881 cgagtgagtc ttcccttgtt attgtgtagc cagaatgccg caaaacttcc atgcctaagc
     2941 gaactgttga gagtacgttt cgatttctga ctgtgttagc ctggaagtgc ttgtcccaac
     3001 cttgtttctg agcatgaacg cccgcaagcc aacatgttag ttgaagcatc agggcgatta
     3061 gcagcatgat atcaaaacgc tctgagctgc tcgttcggct atggcgtagg cctagtccgt
     3121 aggcaggact tttcaagtct cggaaggttt cttcaatctg cattcgcttc gaatagatat
     3181 taacaagttg tttgggtgtt cgaatttcaa caggtaagtt agttgctaga atccatggct
     3241 cctttgccga cgctgagtag attttaggtg acgggtggtg acaatgagtc cgtgtcgagc
     3301 gctgattttt tcggccttta gagcgagatt tatacaatag aatttggcat gagattggat
     3361 tgcttttagt cagcctctta tagcctaaag tctttgagtg actagatgac atatcatgta
     3421 agttgctgat aggtttccag ttttccgctc ctaggtctgc atattgtact tttcctctta
     3481 ctcgacttaa ccagtaccaa cccagcttct caacggattt ataccatggc actttaaagc
     3541 cagcatcact gacaatgagc ggtgtggtgt tactcggtag aatgctcgca aggtcggcta
     3601 gaaattggtc atgagctttc tttgaacatt gctctgaaag cgggaacgct ttctcataaa
     3661 gagtaacaga acgaccgtgt agtgcgactg aagctcgcaa taccataagc cgtttttgct
     3721 cacggatatc agaccagtca acaagtacaa tgggcatcgt attgcccgaa cagataaagc
     3781 tagcatgcca acggtataca gcgagtcgct ctttgtggag gtgacgatta cctaacaatc
     3841 ggtcgattcg tttgatgtta tgttttgttc tcgctttggt tggcaggtta cggccaagtt
     3901 cggtaagagt gagagtttta cagtcaagta aggcgtggca agccaacgtt aagctgttga
     3961 gtcgttttaa gtgtaattcg gggcagaatt ggtaaagaga gtcgtgtaaa atatcgagtt
     4021 cgcacatttt gttgtctgat tattgatttt tggcgaaacc atttgatcat atgacaagat
     4081 gtgtatctac cttaacttaa tgattttgat aaaaatcatt aggggattca tcagtgcaga
     4141 gccaggaatg tatacacagg ggaagaaatc ctccagaaga aagaaattac aatcagagat
     4201 caggaagcac catacctcct gcgaaacctc agtgatcaca cagtggccat cagcagttcc
     4261 accactttag actgtcatgc taatggtgtc cccgagcctc agatcacttg gtttaaaaac
     4321 aaccacaaaa tacaacaaga gcctggaatt attttaggac caggaagcag cacgctgttt
     4381 attgaaagag tcacagaaga ggatgaaggt gtctatcact gcaaagccac caaccagaag
     4441 ggctctgtgg aaagttcagc atacctcact gttcaaggaa cctcggacaa gtctaatctg
     4501 gagctgatca ctctaacatg cacctgtgtg gctgcgactc tcttctggct cctattaacc
     4561 ctctttatcc gaaaaatgaa aaggtcttct tctgaaataa agactgacta cctatcaatt
     4621 ataatggacc cagatgaagt tcctttggat gagcagtgtg agcggctccc ttatgatgcc
     4681 agcaagtggg agtttgcccg ggagagactt aaactgggca aatcacttgg aagaggggct
     4741 tttggaaaag tggttcaagc atcagcattt ggcattaaga aatcacctac gtgccggact
     4801 gtggctgtga aaatgctgaa agagggggcc acggccagcg agtacaaagc tctgatgact
     4861 gagctaaaaa tcttgaccca cattggccac catctgaacg tggttaacct gctgggagcc
     4921 tgcaccaagc aaggagggcc tctgatggtg attgttgaat actgcaaata tggaaatctc
     4981 tccaactacc tcaagagcaa acgtgactta ttttttctca acaaggatgc agcactacac
     5041 atggagccta agaaagaaaa aatggagcca ggcctggaac aaggcaagaa accaagacta
     5101 gatagcgtca ccagcagcga aagctttgcg agctccggct ttcaggaaga taaaagtctg
     5161 agtgatgttg aggaagagga ggattctgac ggtttctaca aggagcccat cactatggaa
     5221 gatctgattt cttacagttt tcaagtggcc agaggcatgg agttcctgtc ttccagaaag
     5281 tgcattcatc gggacctggc agcgagaaac attcttttat ctgagaacaa cgtggtgaag
     5341 atttgtgatt ttggccttgc ccgggatatt tataagaacc ccgattatgt gagaaaagga
     5401 gatactcgac ttcctctgaa atggatggct cccgaatcta tctttgacaa aatctacagc
     5461 accaagagcg acgtgtggtc ttacggagta ttgctgtggg aaatcttctc cttaggtggg
     5521 tctccatacc caggagtaca aatggatgag gacttttgca gtcgcctgag ggaaggcatg
     5581 aggatgagag ctcctgagta ctctactcct gaaatctatc agatcatgct ggactgctgg
     5641 cacagagacc caaaagaaag gccaagattt gcagaacttg tggaaaaact aggtgatttg
     5701 cttcaagcaa atgtacaaca ggatggtaaa gactacatcc caatcaatgc catactgaca
     5761 ggaaatagtg ggtttacata ctcaactcct gccttctctg aggacttctt caaggaaagt
     5821 atttcagctc cgaagtttaa ttcaggaagc tctgatgatg tcagatatgt aaatgctttc
     5881 aagttcatga gcctggaaag aatcaaaacc tttgaagaac ttttaccgaa tgccacctcc
     5941 atgtttgatg actaccaggg cgacagcagc actctgttgg cctctcccat gctgaagcgc
     6001 ttcacctgga ctgacagcaa acccaaggcc tcgctcaaga ttgacttgag agtaaccagt
     6061 aaaagtaagg agtcggggct gtctgatgtc agcaggccca gtttctgcca ttccagctgt
     6121 gggcacgtca gcgaaggcaa gcgcaggttc acctacgacc acgctgagct ggaaaggaaa
     6181 atcgcgtgct gctccccgcc cccagactac aactcggtgg tcctgttctc caccccaccc
     6241 atcacgcgta cgcggccgct cgagcagaaa ctcatctcag aagaggatct ggcagcaaat
     6301 gatatcctgg attacaagga tgacgacgat aaggtttaaa cggccggccg cggtcatagc
     6361 tgtttcctga acagatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     6421 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     6481 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     6541 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     6601 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     6661 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     6721 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     6781 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     6841 tcccttccct gtccttctga ttttaaaata actataccag caggaggacg tccagacaca
     6901 gcataggcta cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt
     6961 cctctcatgc gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc
     7021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7141 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7201 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7261 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7321 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7381 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7441 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7501 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7561 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7621 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7681 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7741 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7801 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7861 gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg
     7921 ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa
     7981 aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc
     8041 agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct
     8101 gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag
     8161 ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca
     8221 tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg
     8281 aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag
     8341 tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     8401 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     8461 atcacgggta gccaacgcta tgtcctgata gcgatccgcc acacccagcc ggccacagtc
     8521 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     8581 ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc
     8641 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     8701 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     8761 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     8821 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     8881 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     8941 tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     9001 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     9061 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     9121 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc
     9181 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg
     9241 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg
     9301 aactgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
     9361 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat
     9421 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
     9481 acacattcca cagctggttc tttccgcctc aggactcttc ctttttcaat attattgaag
     9541 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9601 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     9661 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     9721 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     9781 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     9841 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     9901 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     9961 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    10021 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
    10081 aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
    10141 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    10201 ttaagttggg taacgccagg gttttcccag tcacgacgt
//
