LOCUS       sequence-181549-        3117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  47xCAG repeats in pBluescript vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Jain A, Vale RD
  TITLE     RNA phase transitions in repeat expansion disorders.
  JOURNAL   Nature. 2017 Jun 8;546(7657):243-247. doi: 10.1038/nature22386. Epub
            2017 May 31.
  PUBMED    28562589
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature22386"; journalName: "Nature"; date: "2017-06-8- 8"; 
            volume: "546"; issue: "7657"; pages: "243-247"
COMMENT     Sequence Label: pBluescript-CTG-47
FEATURES             Location/Qualifiers
     source          1..3117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(305..325)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(305..323)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(344..360)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(344..360)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(357..379)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    368..384
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(392..398,399..416,417..422))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    437..458
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(575..592)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(746..1334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(826..845)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1505..2296,2297..2365))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2128..2147
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2366..2470)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2496..2951)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2633..2654)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2845..2864
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3078..3100
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3092..3109
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3093..3109
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc gcgtcgacga
       61 gctccgtctc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
      121 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
      181 ctgctgctgc tgctgctgct gctgctgctg tgagaccgaa ttcgatatca agctttagct
      241 agctaactag ctaactaggc tcttccggcc tctcgagggg aggcccggta cccagctttt
      301 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
      361 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      421 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      481 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      541 gaggcggttt gcgtattggg cgcacttccg cttcctcgct cactgactcg ctgcgctcgg
      601 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      661 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      721 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      781 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      841 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      901 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      961 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1021 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1081 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1141 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     1201 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1261 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1321 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1381 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1441 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1501 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1561 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1621 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1681 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1741 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1801 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1861 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1921 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1981 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2041 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2101 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2161 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2221 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2281 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2341 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2401 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2461 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     2521 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     2581 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     2641 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     2701 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     2761 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     2821 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     2881 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     2941 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     3001 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     3061 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgc
//
