LOCUS       sequence-181550-       10436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd 
            generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Jain A, Vale RD
  TITLE     RNA phase transitions in repeat expansion disorders.
  JOURNAL   Nature. 2017 Jun 8;546(7657):243-247. doi: 10.1038/nature22386. Epub
            2017 May 31.
  PUBMED    28562589
REFERENCE   2  (bases 1 to 10436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature22386"; journalName: "Nature"; date: "2017-06-8- 8"; 
            volume: "546"; issue: "7657"; pages: "243-247"
COMMENT     Sequence Label: pHR-tdMCP-YFP
FEATURES             Location/Qualifiers
     source          1..10436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      834..1422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1323..1342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1576..1593
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1663..1683)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1668..1997
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1848..1983
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          3131..3196
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3326..3346
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3771..3905
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3808..3827)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3862..3881
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4074..4707
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4754..4879
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5371..5604
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5789..5833
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6116..6233
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6386..6793
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      6827..6836
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7589..7591,7592..8305)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7631..7652)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7892..7911)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8239..8260
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8371..8959
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8424..8444)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8842..8853)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8962..8978)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8963..8979)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9069..9302
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9327..9345)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9328..9345)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9635..9654)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     9988..10010
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10048..10066)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10134..10238
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10239..10307,10308..10436,1..663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1441 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     1561 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     1621 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag
     1681 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     1741 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     1801 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     1861 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     1921 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     1981 gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata
     2041 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     2101 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     2161 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     2221 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     2281 tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg
     2341 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
     2401 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
     2461 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
     2521 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
     2581 tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
     2641 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
     2701 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
     2761 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
     2821 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
     2881 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
     2941 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
     3001 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt
     3061 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     3121 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     3181 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     3241 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     3301 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     3361 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     3421 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     3481 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
     3541 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
     3601 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
     3661 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     3721 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     3781 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3841 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3901 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     3961 ttaccactgc caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta
     4021 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg
     4081 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg
     4141 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
     4201 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
     4261 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     4321 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
     4381 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
     4441 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
     4501 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4561 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4621 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4681 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     4741 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     4801 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     4861 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     4921 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     4981 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     5041 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     5101 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     5161 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     5221 cggtgatctt cagacctgga cgatatatat gagggacaat tggagaagtg aattatataa
     5281 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     5341 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     5401 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     5461 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     5521 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     5581 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     5641 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     5701 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     5761 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     5821 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     5881 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     5941 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     6001 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     6061 cattcgatta gtgaacggat ctcgacggtc gccaaatggc agtattcatc cacaatttta
     6121 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     6181 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     6241 attacaggga cagcagagat ccagtttgga tcgataagct tgatatcgaa ttcctgcagc
     6301 cccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     6361 ctgtaggttt ggcaagctag ctgcagtaac gccattttgc aaggcatgga aaaataccaa
     6421 accaagaata gagaagttca gatcaagggc gggtacatga aaatagctaa cgttgggcca
     6481 aacaggatat ctgcggtgag cagtttcggc cccggcccgg ggccaagaac agatggtcac
     6541 cgcagtttcg gccccggccc gaggccaaga acagatggtc cccagatatg gcccaaccct
     6601 cagcagtttc ttaagaccca tcagatgttt ccaggctccc ccaaggacct gaaatgaccc
     6661 tgcgccttat ttgaattaac caatcagcct gcttctcgct tctgttcgcg cgcttctgct
     6721 tcccgagctc tataaaagag ctcacaaccc ctcactcggc gcgccagtcc tccgacagac
     6781 tgagtcgccc gggggggatc tggagctctc gagaattctc acgcgtgcca ccatggccag
     6841 caacttcacc cagttcgtgc tggtggacaa cggcggcacc ggcgacgtga ccgtggcccc
     6901 cagcaacttc gccaacggca tcgccgagtg gatcagcagc aacagccgca gccaggccta
     6961 caaggtgacc tgcagcgtgc gccagagcag cgcccagaac cgcaagtaca ccatcaaggt
     7021 ggaggtgccc aagggcgcct ggcgcagcta cctgaacatg gagctgacca tccccatctt
     7081 cgccaccaac agcgactgcg agctgatcgt gaaggccatg cagggcctgc tgaaggacgg
     7141 caaccccatc cccagcgcca tcgccgccaa cagcggcatc tacggcggcg ctggaggtgc
     7201 aggtggatcc atggccagca acttcaccca gttcgtgctg gtggacaacg gcggcaccgg
     7261 cgacgtgacc gtggccccca gcaacttcgc caacggcatc gccgagtgga tcagcagcaa
     7321 cagccgcagc caggcctaca aggtgacctg cagcgtgcgc cagagcagcg cccagaaccg
     7381 caagtacacc atcaaggtgg aggtgcccaa gggcgcctgg cgcagctacc tgaacatgga
     7441 gctgaccatc cccatcttcg ccaccaacag cgactgcgag ctgatcgtga aggccatgca
     7501 gggcctgctg aaggacggca accccatccc cagcgccatc gccgccaaca gcggcatcta
     7561 cggcggcgct ggaggtgcag gtggatccgt gagcaagggc gaggagctgt tcaccggggt
     7621 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     7681 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     7741 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt
     7801 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     7861 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     7921 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     7981 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     8041 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     8101 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     8161 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaaactga gcaaagaccc
     8221 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     8281 cggcatggac gagctgtaca agtgatgaat tgcggccgcg actctagagt cgacctgcag
     8341 gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     8401 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     8461 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8521 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8581 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8641 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8701 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8761 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8821 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8881 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8941 ctttgggccg cctccccgca tcgataccgt cgacctcgag ggaattaatt cgagctcggt
     9001 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     9061 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     9121 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     9181 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     9241 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     9301 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     9361 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     9421 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     9481 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     9541 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     9601 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     9661 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     9721 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     9781 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     9841 ggagagcgtc gaatggtgca ctctcagtac aatctagctc tgatgccgca tagttaagcc
     9901 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     9961 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
    10021 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
    10081 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
    10141 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    10201 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    10261 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    10321 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    10381 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatg
//
