LOCUS       sequence-18157-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Veldman T, Etheridge KT, Counter CM
  TITLE     Loss of hPot1 function leads to telomere instability and a cut-like 
            phenotype.
  JOURNAL   Curr Biol. 2004 Dec 29. 14(24):2264-70.
  PUBMED    15620654
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2004 Dec 29. 14(24):2264-70."
COMMENT     Sequence Label: PSuperPuroRetro-1660s(hPot1)
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..71
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     56..72
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(165..379)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(225..244)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        390..889
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(425..448)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     822..841
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ctactcctta tcagccctca ctccttctct aggcgccgga attagatcga tctctcgagg
       61 tcgacggtat cgataggctt ctagtacttt ttggaaaagc ttttccaaaa agtactagag
      121 cctatctcat ccttgaatga gataggcttc tagtacgggg atctgtggtc tcatacagaa
      181 cttataagat tcccaaatcc aaagacattt cacgtttatg gtgatttccc agaacacata
      241 gcgacatgca aatattgcag ggcgccactc ccctgtccct cacagccatc ttcctgccag
      301 ggcgcacgcg cgctgggtgt tcccgcctag tgacactggg cccgcgattc cttggagcgg
      361 gttgatgacg tcagcgttcg aattctaccg ggtaggggag gcgcttttcc caaggcagtc
      421 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
      481 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
      541 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
      601 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
      661 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
      721 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
      781 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
      841 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
      901 aagctagctt accatgaccg agtacaagcc caacgg
//
