LOCUS       sequence-181574-       10354 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral overexpression vector to make stable bicistronic cell 
            line for alpha-Syn screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10354)
  AUTHORS   Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J, 
            Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L,
            Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY
  TITLE     TRIM28 regulates the nuclear accumulation and toxicity of both 
            alpha-synuclein and tau.
  JOURNAL   Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809.
  PUBMED    27779468
REFERENCE   2  (bases 1 to 10354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-10-25"; volume: "5. pii: e19809. doi"; pages: " 
            10.7554/eLife.19809"
COMMENT     Sequence Label: pLenti-DsRed_IRES_SNCA:EGFP
FEATURES             Location/Qualifiers
     source          1..10354
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             376..633
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2057..2174
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2226..2529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2530..2733
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2684..2704
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2730..2754
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2837..2846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2843..3520
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(2973..2991)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(3023..3043)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3241..3260
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3435..3458
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3546..4119
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3569..4121
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3713..3730)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3940..3959
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4571..4573,4574..4576,4577..5290)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4571..4573,4574..4576,4577..5287)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4616..4637)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4877..4896)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5224..5245
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5310..5329)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5311..5329)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5374..5397)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5747..5766
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5835..6629
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5889..5908)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6499..6518
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6743..7331
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6796..6816)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7214..7225)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7406..7639
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7758..7776)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7844..7948
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7949..8017,8018..8809)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8167..8186)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8980..9568
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9469..9488
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9722..9739
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9979..10000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10015..10020,10021..10038,10039..10045)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10053..10069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10058..10080
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10077..10093
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10077..10093
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10143..10162
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat tcacaaatgg
     2041 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2101 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2161 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg actagtggag
     2221 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2281 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2341 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2401 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2461 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2521 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     2581 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     2641 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     2701 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     2761 agacgccatc cacgctgttt tgacctccat agaagacacc ggcggcctag cgctaccgga
     2821 ctcagatcca ccggtcgcca ccatggcctc ctccgaggac gtcatcaagg agttcatgcg
     2881 cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     2941 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
     3001 cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt
     3061 gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg
     3121 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     3181 gcaggacggc tccttcatct acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg
     3241 ccccgtaatg cagaagaaga ctatgggctg ggaggcctcc accgagcgcc tgtacccccg
     3301 cgacggcgtg ctgaagggcg agatccacaa ggccctgaag ctgaaggacg gcggccacta
     3361 cctggtggag ttcaagtcca tctacatggc caagaagccc gtgcagctgc ccggctacta
     3421 ctacgtggac tccaagctgg acatcacctc ccacaacgag gactacacca tcgtggagca
     3481 gtacgagcgc gccgagggcc gccaccacct gttcctgtag cggccaattc tgcagtcgac
     3541 atccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     3601 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     3661 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     3721 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     3781 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     3841 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     3901 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     3961 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     4021 cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     4081 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa tcgatatgga
     4141 tgtattcatg aaaggacttt caaaggccaa ggagggagtt gtggctgctg ctgagaaaac
     4201 caaacagggt gtggcagaag cagcaggaaa gacaaaagag ggtgttctct atgtaggctc
     4261 caaaaccaag gagggagtgg tgcatggtgt ggcaacagtg gctgagaaga ccaaagagca
     4321 agtgacaaat gttggaggag cagtggtgac gggtgtgaca gcagtagccc agaagacagt
     4381 ggagggagca gggagcattg cagcagccac tggctttgtc aaaaaggacc agttgggcaa
     4441 gaatgaagaa ggagccccac aggaaggaat tctggaagat atgcctgtgg atcctgacaa
     4501 tgaggcttat gaaatgcctt ctgaggaagg gtatcaagac tacgaacctg aagccctcga
     4561 ggcggccgct atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     4621 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     4681 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     4741 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     4801 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     4861 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     4921 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     4981 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     5041 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     5101 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     5161 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     5221 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     5281 gtacaagtaa tctagataac tgatcctctc cctatagtga gtcgtattaa attctaccgg
     5341 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     5401 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     5461 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     5521 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     5581 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     5641 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     5701 gggtccgggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     5761 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     5821 agcccaagct taccatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     5881 tggagaggct attcggctat gactgggcac aacagacgat cggctgctct gatgccgccg
     5941 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     6001 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     6061 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     6121 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     6181 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     6241 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     6301 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     6361 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     6421 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     6481 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     6541 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     6601 tctatcgcct tcttgacgag ttcttctgac ctgaggatca taatcagcca taccacattt
     6661 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     6721 atgaatgcaa ttgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac
     6781 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6841 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6901 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6961 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7021 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7081 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7141 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7201 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7261 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7321 cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc
     7381 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     7441 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7501 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7561 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7621 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     7681 gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca
     7741 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     7801 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7861 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7921 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7981 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     8041 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     8101 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     8161 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     8221 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8281 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8341 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8401 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8461 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8521 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8581 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8641 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8701 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8761 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8821 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8881 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8941 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9001 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9061 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9121 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9181 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9241 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9301 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9361 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9421 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9481 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9541 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9601 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9661 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     9721 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     9781 gcgttggccg attcattaat gcagcaagct catggctgac taattttttt tatttatgca
     9841 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9901 ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg
     9961 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
    10021 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
    10081 aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag
    10141 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    10201 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    10261 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    10321 tatttgttaa atatgtacta caaacttagt agtt
//
