LOCUS       sequence-181579-        9131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of human Igfbp2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Vo BT, Li C, Morgan MA, Theurillat I, Finkelstein D, Wright S, Hyle 
            J, Smith SM, Fan Y, Wang YD, Wu G, Orr BA, Northcott PA, Shilatifard
            A, Sherr CJ, Roussel MF
  TITLE     Inactivation of Ezh2 Upregulates Gfi1 and Drives Aggressive 
            Myc-Driven Group 3 Medulloblastoma.
  JOURNAL   Cell Rep. 2017 Mar 21;18(12):2907-2917. doi: 
            10.1016/j.celrep.2017.02.073.
  PUBMED    28329683
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.02"; journalName: "Cell Rep"; date: 
            "2017-03-21- 21"; volume: "18"; issue: "12"; pages: "2907-2917"
COMMENT     Sequence Label: MSCV-human Igf2bp2-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..39
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     167..186
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             386..902
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    966..1307
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1374..1790
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1720..1742
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1758..1774
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        2021..2400
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2401..2604
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2555..2575
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2649..2668
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2649..2667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    4478..5027
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4622..4639)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4849..4868
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5030..5032,5033..5035,5036..5749)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5030..5032,5033..5035,5036..5746)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5075..5096)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5336..5355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5683..5704
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5911..6425
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6587..6603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6611..6617,6618..6635,6636..6641))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6656..6677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6794..6811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6965..7553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7045..7064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7724..8515,8516..8584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8347..8366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8585..8689)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8757..8775
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8813..8835)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat
       61 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
      121 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
      181 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
      241 agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
      301 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
      361 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
      421 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
      481 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
      541 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
      601 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
      661 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
      721 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
      781 gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggac
      841 tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg gggtctttca
      901 tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc cccccgccgg
      961 gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt gtttgtgccg
     1021 gcatctaatg tttgcgcctg cgtctgtact agttagctaa ctagctctgt atctggcgga
     1081 cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg
     1141 actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc
     1201 cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     1261 aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca
     1321 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tagggccaga
     1381 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
     1441 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
     1501 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
     1561 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
     1621 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
     1681 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
     1741 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattggga
     1801 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1861 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     1921 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     1981 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     2041 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     2101 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     2161 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2221 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2281 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2341 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2401 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2461 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2521 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2581 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
     2641 atcgaaatta atacgactca ctatagggag acccaagctg gctagttaag cttggtaccg
     2701 agctcggatc cactagtcca gtgtggtgga attgcccttg atgatgaaca agctttacat
     2761 cgggaacctg agccccgccg tcaccgccga cgacctccgg cagctctttg gggacaggaa
     2821 gctgcccctg gcgggacagg tcctgctgaa gtccggctac gccttcgtgg actaccccga
     2881 ccagaactgg gccatccgcg ccatcgagac cctctcgggt aaagtggaat tgcatgggaa
     2941 aatcatggaa gttgattact cagtctctaa aaagctaagg agcaggaaaa ttcagattcg
     3001 aaacatccct cctcacctgc agtgggaggt gttggatgga cttttggctc aatatgggac
     3061 agtggagaat gtggaacaag tcaacacaga cacagaaacc gccgttgtca acgtcacata
     3121 tgcaacaaga gaagaagcaa aaatagccat ggagaagcta agcgggcatc agtttgagaa
     3181 ctactccttc aagatttcct acatcccgga tgaagaggtg agctcccctt cgccccctca
     3241 gcgagcccag cgtggggacc actcttcccg ggagcaaggc cacgcccctg ggggcacttc
     3301 tcaggccaga cagattgatt tcccgctgcg gatcctggtc cccacccagt ttgttggtgc
     3361 catcatcgga aaggagggct tgaccataaa gaacatcact aagcagaccc agtcccgggt
     3421 agatatccat agaaaagaga actctggagc tgcagagaag cctgtcacca tccatgccac
     3481 cccagagggg acttctgaag catgccgcat gattcttgaa atcatgcaga aagaggcaga
     3541 tgagaccaaa ctagccgaag agattcctct gaaaatcttg gcacacaatg gcttggttgg
     3601 aagactgatt ggaaaagaag gcagaaattt gaagaaaatt gaacatgaaa cagggaccaa
     3661 gataacaatc tcatctttgc aggatttgag catatacaac ccggaaagaa ccatcactgt
     3721 gaagggcaca gttgaggcct gtgccagtgc tgagatagag attatgaaga agctgcgtga
     3781 ggcctttgaa aatgatatgc tggctgttaa cacccactcc ggatacttct ccagcctgta
     3841 cccccatcac cagtttggcc cgttcccgca tcatcactct tatccagagc aggagattgt
     3901 gaatctcttc atcccaaccc aggctgtggg cgccatcatc gggaagaagg gggcacacat
     3961 caaacagctg gcgagattcg ccggagcctc tatcaagatt gcccctgcgg aaggcccaga
     4021 cgtcagcgaa aggatggtca tcatcaccgg gccaccggaa gcccagttca aggcccaggg
     4081 acggatcttt gggaaactga aagaggaaaa cttctttaac cccaaagaag aagtgaagct
     4141 ggaagcgcat atcagagtgc cctcttccac agctggccgg gtgattggca aaggtggcaa
     4201 gaccgtgaac gaactgcaga acttaaccag tgcagaagtc atcgtgcctc gtgaccaaac
     4261 gccagatgaa aatgaggaag tgatcgtcag aattatcggg cacttctttg ctagccagac
     4321 tgcacagcgc aagatcaggg aaattgtaca acaggtgaag cagcaggagc agaaataccc
     4381 tcagggagtc gcctcacagc gcagcaagaa gggcaattct gcagatatcc agcacagtgg
     4441 cggccgctcg agcgggatca attccgcccc ccccctaacg ttactggccg aagccgcttg
     4501 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     4561 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     4621 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     4681 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     4741 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     4801 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     4861 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     4921 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     4981 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca tggtgagcaa
     5041 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     5101 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     5161 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     5221 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     5281 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     5341 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     5401 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     5461 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     5521 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5581 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     5641 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5701 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga
     5761 ctctagagtc gacctgcagg catgcaagct tcaggtagcc ggctaacgtt aacaaccggt
     5821 acctctagaa ctatagctag catgcgcaaa tttaaagcgc tgatatcgat aaaataaaag
     5881 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     5941 ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt
     6001 tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt
     6061 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc
     6121 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     6181 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     6241 tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgataga
     6301 ctgcgtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     6361 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     6421 ttcatgggta acagtttctt gaagttggag aacaacattc tgagggtagg agtcgaatat
     6481 taagtaatcc tgactcaatt agccactgtt ttgaatccac atactccaat actcctgaaa
     6541 tagttcatta tgaacagcgc agaaagagct ggggagaatt gtgaaattgt tatccgctca
     6601 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6661 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6721 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7381 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8341 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8581 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8641 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8701 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     8761 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     8821 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     8881 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     8941 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     9001 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     9061 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     9121 gattaagttg g
//
