LOCUS       sequence-181583-        8833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral expression of mouse Gfi1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Vo BT, Li C, Morgan MA, Theurillat I, Finkelstein D, Wright S, Hyle 
            J, Smith SM, Fan Y, Wang YD, Wu G, Orr BA, Northcott PA, Shilatifard
            A, Sherr CJ, Roussel MF
  TITLE     Inactivation of Ezh2 Upregulates Gfi1 and Drives Aggressive 
            Myc-Driven Group 3 Medulloblastoma.
  JOURNAL   Cell Rep. 2017 Mar 21;18(12):2907-2917. doi: 
            10.1016/j.celrep.2017.02.073.
  PUBMED    28329683
REFERENCE   2  (bases 1 to 8833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.02"; journalName: "Cell Rep"; date: 
            "2017-03-21- 21"; volume: "18"; issue: "12"; pages: "2907-2917"
COMMENT     Sequence Label: pCDH-CMV-mouse Gfi1-GFP
FEATURES             Location/Qualifiers
     source          1..8833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     97..119
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     111..128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     112..128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        143..369
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             370..550
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    597..722
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1215..1448
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1633..1677
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1944..2060
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2150..2353
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2304..2324
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2350..2374
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3754..4303
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3898..3915)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4125..4144
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4306..4308,4309..4311,4312..5025)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4306..4308,4309..4311,4312..5022)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4351..4372)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4612..4631)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4959..4980
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5050..5637
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5102..5122)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5520..5531)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5711..5944
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6016..6137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6053..6072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6107..6126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6177..6312
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6345..6361)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6345..6361)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6358..6380)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6369..6385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6393..6399,6400..6417,6418..6423))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6438..6459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6576..6593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6747..7335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7506..8297,8298..8366))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8129..8148
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8367..8471)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8539..8557
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8595..8617)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8717..8736
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
       61 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
      121 cggccagtgc caagctgacg cgtgtagtct tatgcaatac tcttgtagtc ttgcaacatg
      181 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
      241 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
      301 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
      361 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      421 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
      481 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
      541 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
      601 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
      661 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
      721 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
      781 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
      841 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
      901 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
      961 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     1021 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccactgat cttcagacct
     1081 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1141 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1201 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1261 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1321 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1381 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1441 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1501 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     1561 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     1621 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     1681 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     1741 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     1801 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     1861 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     1921 ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca
     1981 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     2041 attacaaaat tcaaaatttt atcgatacta gtattatgcc cagtacatga ccttatggga
     2101 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2161 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2221 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2281 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggttta
     2341 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     2401 tgacctccat agaagattct agagctagcg aattcgatat caccggtacc atgccgcgct
     2461 cattcctggt caagagcaag aaggcgcaca gctatcacca gccgcgttct ccggggccgg
     2521 actactccct gcgcctggag accgtgcctg cgccgggcag agcagagggc ggcgctgtga
     2581 gtgcaggcga gtcgaaaatg gagccccgag agcgtttgtc ccccgactct cagcttaccg
     2641 aggctcccga cagggcctcc gcgtccccca acagctgcga aggcagcgtt tgtgacccct
     2701 gctccgagtt cgaggacttt tggaggcccc cttctccctc cgtgtctcca gcgtcggaga
     2761 agtcactgtg ccgctctctg gacgaagccc agccctacac gctgcctttc aagccctatg
     2821 catggagcgg tcttgctggg tctgacctgc ggcacctggt gcagagctat cggcagtgca
     2881 gcgcgctgga gcgcagcgcg ggcctgagcc tcttctgcga gcgcggctcg gagccgggcc
     2941 gcccggcagc gcgctacggc cccgagcagg ctgcgggcgg agccggtgcg ggacagccag
     3001 ggagctgcgg ggtcgccggg ggcgccacca gcgctgcggg cctggggctc tacggcgact
     3061 tcgcgcctgc ggcggccggg ctgtacgagc ggccgagcac agcagcaggc cggctgtacc
     3121 aagatcatgg ccacgagctg cacgcggaca agagcgtggg cgtcaaggtg gagtcggagc
     3181 tgctttgcac ccgtctgctg ctgggcggcg gctcctacaa atgcatcaaa tgcagcaagg
     3241 tgttctccac accgcacggg ctggaggtgc acgtgcgccg gtcccacagc ggcacaagac
     3301 cctttgcgtg cgagatgtgc ggcaagacct tcgggcacgc ggtgagcctg gagcaacaca
     3361 aggcagtgca ctcccaggaa cgcagctttg actgtaagat ctgtggcaag agcttcaaga
     3421 ggtcatccac gctgtccaca catctgctca ttcactcgga cacccggccc tatccctgtc
     3481 agtactgtgg caaaagattc caccagaagt cagatatgaa gaaacacacc ttcatccaca
     3541 caggtgagaa gccccacaaa tgccaggtgt gcggcaaagc cttcagtcag agctccaacc
     3601 tcatcactca tagcagaaag cacacaggct tcaagccctt tggctgtgac ctgtgtggga
     3661 agggcttcca gaggaaggtg gatctcagga ggcaccgaga gactcagcat ggactcaaat
     3721 gactcgagcg ggatcaattc cgcccccccc ctaacgttac tggccgaagc cgcttggaat
     3781 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     3841 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     3901 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     3961 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     4021 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     4081 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     4141 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     4201 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     4261 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatggt gagcaagggc
     4321 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     4381 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     4441 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     4501 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     4561 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     4621 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     4681 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     4741 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     4801 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     4861 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     4921 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     4981 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     5041 agagtcgaca atcaacctct gattacaaaa tttgtgaaag attgactggt attcttaact
     5101 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5161 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5221 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5281 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5341 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5401 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     5461 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5521 cggccctcaa tccagcggac cttccttccc gcgccctgct gccggctctg cggcctcttc
     5581 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     5641 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5701 aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct ttttgcttgt
     5761 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5821 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5881 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5941 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     6001 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6061 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6121 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     6181 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6241 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     6301 tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata gctgtttcct
     6361 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6421 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6481 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6541 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6601 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6661 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6721 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6781 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6841 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6901 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6961 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7021 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7081 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7141 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7201 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7261 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7321 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7381 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7441 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7501 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7561 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7621 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7681 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7741 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7801 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7861 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7921 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7981 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8041 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8101 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8161 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8221 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8281 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8341 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8401 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8461 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     8521 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     8581 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     8641 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     8701 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     8761 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     8821 ctgcgcaact gtt
//
