LOCUS       sequence-181588-       10623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral overexpression vector (doxycycline-inducible) for 
            alpha-Syn.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J, 
            Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L,
            Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY
  TITLE     TRIM28 regulates the nuclear accumulation and toxicity of both 
            alpha-synuclein and tau.
  JOURNAL   Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809.
  PUBMED    27779468
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-10-25"; volume: "5. pii: e19809. doi"; pages: " 
            10.7554/eLife.19809"
COMMENT     Sequence Label: pINDUCER20-alpha-Syn
FEATURES             Location/Qualifiers
     source          1..10623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3161..3190
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(3210..3234)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3287..3313
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3399..4610
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4447..4466
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4669..5286,5287..5292,5293..5328,5329..5331,5332..5367,
                     5368..5370,5371..5406,5407..5415)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5453..6026
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5620..5637)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5847..5866
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6042..6836
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6042..6836
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6096..6115)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6706..6725
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7013..7601
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7066..7086)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7484..7495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7676..7909
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8028..8046)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8114..8218
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8219..8287,8288..9079)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8437..8456)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9250..9838
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9739..9758
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9992..10009
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10249..10270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10285..10290,10291..10308,10309..10315)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10323..10339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10328..10350
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10347..10363
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10347..10363
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10413..10432
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctaagct tgctcgaggg atggatgtat tcatgaaagg
     2761 actttcaaag gccaaggagg gagttgtggc tgctgctgag aaaaccaaac agggtgtggc
     2821 agaagcagca ggaaagacaa aagagggtgt tctctatgta ggctccaaaa ccaaggaggg
     2881 agtggtgcat ggtgtggcaa cagtggctga gaagaccaaa gagcaagtga caaatgttgg
     2941 aggagcagtg gtgacgggtg tgacagcagt agcccagaag acagtggagg gagcagggag
     3001 cattgcagca gccactggct ttgtcaaaaa ggaccagttg ggcaagaatg aagaaggagc
     3061 cccacaggaa ggaattctgg aagatatgcc tgtggatcct gacaatgagg cttatgaaat
     3121 gccttctgag gaagggtatc aagactacga acctgaagcc gagcagaagc tgatcagcga
     3181 ggaggacctg tgagcccggt gcggccgcaa cccagctttc ttgtacaaag tggttgatat
     3241 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaatacc catacgacgt
     3301 cccagactac gcttagtaat gattaattaa actagaaatt ctaccgggta ggggaggcgc
     3361 ttttcccaag gcagtctgga gtaacccacc caagatctgg cctccgcgcc gggttttggc
     3421 gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag
     3481 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
     3541 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
     3601 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
     3661 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
     3721 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
     3781 ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc
     3841 gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg
     3901 cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc
     3961 gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg
     4021 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
     4081 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
     4141 ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt
     4201 gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg
     4261 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
     4321 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
     4381 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
     4441 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
     4501 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
     4561 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac gaagcttggt
     4621 accgagctcg gatctccacc ccgtaccggt cctgcagtcg aattcaccat gtctagactg
     4681 gacaagagca aagtcataaa cggagctctg gaattactca atggtgtcgg tatcgaaggc
     4741 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac
     4801 gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat
     4861 acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag
     4921 tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc
     4981 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc
     5041 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc
     5101 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat
     5161 tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct
     5221 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa
     5281 agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     5341 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac
     5401 atgctccccg ggtaactaag taaggatccg cggccgcact agaggaattc cgcccctctc
     5461 cccccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     5521 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5581 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5641 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5701 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5761 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5821 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5881 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5941 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     6001 ttttcctttg aaaaacacga tgataagctt accggtccac catgattgaa caagatggat
     6061 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6121 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6181 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     6241 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6301 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6361 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6421 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6481 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6541 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     6601 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6661 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6721 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6781 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgatgta
     6841 caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     6901 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     6961 gttgttgttt agtccctccc aattcgatat caagcttatc gcgatagatc ctaatcaacc
     7021 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7081 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7141 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7201 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7261 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7321 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7381 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     7441 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7501 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7561 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca
     7621 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     7681 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     7741 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7801 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7861 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     7921 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     7981 cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg
     8041 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     8101 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8161 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8221 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8281 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8341 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8401 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8461 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8521 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8581 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8641 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     8701 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     8761 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     8821 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     8881 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     8941 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     9001 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     9061 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     9121 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9181 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9241 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     9301 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9361 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     9421 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9481 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     9541 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     9601 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     9661 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     9721 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     9781 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     9841 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     9901 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     9961 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    10021 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct
    10081 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    10141 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg
    10201 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    10261 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    10321 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac
    10381 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10441 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    10501 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    10561 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    10621 agt
//
