LOCUS       sequence-181607-        8992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector to introduce an osTir1 expressing cassette at the 
            mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron
            system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur 
            N, Dekker J, Mirny LA, Bruneau BG
  TITLE     Targeted Degradation of CTCF Decouples Local Insulation of 
            Chromosome Domains from Genomic Compartmentalization.
  JOURNAL   Cell. 2017 May 18;169(5):930-944.e22. doi: 
            10.1016/j.cell.2017.05.004.
  PUBMED    28525758
REFERENCE   2  (bases 1 to 8992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.05"; journalName: "Cell"; date: "2017-05-18- 
            18"; volume: "169"; issue: "5"; pages: "930-944.e22"
COMMENT     Sequence Label: pEN114 -
            pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-...
FEATURES             Location/Qualifiers
     source          1..8992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     235..257
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     319..338
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             397..2121
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             2122..2163
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(2254..2273)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2319..2374
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2373..2392)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2735..2751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2759..2775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2783..2789,2790..2807,2808..2813))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2828..2849
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    2909..2942
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        3006..3505
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3041..3064)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3438..3457
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        3517..3564
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3583..4182
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3583..3602)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4079..4099
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4214..4231)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4220..4444
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4499..4532
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        complement(5358..5376)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5397..5413)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5421..5437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5445..5451,5452..5469,5470..5475))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5490..5511
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5628..5645)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5799..6387)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5879..5898)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6558..7349,7350..7418))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7181..7200
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7419..7523)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7549..8004)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7686..7707)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7898..7917
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8131..8153
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8145..8162
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8146..8162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8169..8188
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8169..8187
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
       61 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
      121 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
      181 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
      241 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
      301 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
      361 aattcctcga gaattctcac gcgtctgcag gatatcatga catacttccc tgaggaggtg
      421 gtcgaacata ttttctcatt ccttcccgcc caacgagata ggaataccgt gtccctggtg
      481 tgcaaggtct ggtatgagat cgaacgcttg agccgcagag gtgtttttgt gggtaattgt
      541 tatgccgtac gagccgggcg agtggccgcc cggttcccga acgtcagggc ccttacagtg
      601 aagggcaagc ctcactttgc cgacttcaat ttggtgcctc cggactgggg cggctacgcc
      661 gggccctgga tcgaggccgc tgcgcgcggg tgtcatggtc tggaagaact caggatgaaa
      721 aggatggtgg tgtcagacga gtcactggaa ctgcttgccc gcagcttccc ccgattccgc
      781 gccctggtgc tgatcagctg tgaaggattt agcaccgacg gtctggctgc tgtagctagc
      841 cattgtaaac tgctgcgaga actggacctg caggaaaatg aggtagagga taggggacct
      901 cggtggctga gctgtttccc ggatagctgc actagcctgg tgagcttgaa tttcgcttgt
      961 atcaagggcg aagtgaatgc cgggagtttg gagagactcg tttcccgatc ccccaacctg
     1021 agatctctgc ggctgaacag atccgtttct gtggacacgc tcgccaagat tttgctccgg
     1081 actcccaacc tggaggatct ggggaccgga aatctgaccg atgatttcca gacagagagc
     1141 tactttaagc tcactagtgc tctggaaaaa tgcaaaatgc ttaggtcact gagcgggttt
     1201 tgggacgcta gccctgtctg cctgtctttt atatatcccc tgtgtgcaca gttgaccgga
     1261 cttaatctga gttacgctcc aaccctcgac gctagtgacc ttactaagat gatcagccgc
     1321 tgcgtgaagc tccaaaggct ctgggtactg gattgtattt cagacaaggg actgcaagtt
     1381 gtggcctcct cctgcaaaga tctgcaggag ctgagggtgt tcccttcaga tttctatgtg
     1441 gccggataca gcgcagtcac cgaggaggga ttggtagcag tgtccctggg gtgccccaaa
     1501 ctcaatagtt tgctgtactt ctgtcatcag atgaccaatg ctgcccttgt taccgttgcc
     1561 aaaaactgtc caaacttcac aaggtttaga ctgtgcatct tggagcccgg taagcccgac
     1621 gtcgtaacgt cacagccact ggacgaaggg ttcggtgcca tagtacgcga atgtaagggt
     1681 ctccagcggc tgagcatatc cggcctgctg actgacaaag tgttcatgta tattgggaag
     1741 tacgccaagc agctggaaat gctgtcaatc gcattcgccg gagattcaga taaagggatg
     1801 atgcacgtga tgaacgggtg caagaacctg agaaaactcg aaattcgcga ttcccctttt
     1861 ggggacgccg cactgctggg caatttcgcc cggtacgaga cgatgaggtc actttggatg
     1921 tcaagctgca acgtgaccct caagggatgc caagtcctgg ctagtaaaat gccaatgctg
     1981 aatgttgagg tcatcaacga acgcgacggt agtaatgaaa tggaggaaaa ccacggcgat
     2041 ctgcccaaag tggagaaact gtacgtctat cggaccacag ctggcgcacg agatgacgct
     2101 cccaatttcg tgaaaattct gggcaagccc atccccaacc ccctgctggg cctggacagc
     2161 acctgacccg ggtaagcggc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
     2221 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
     2281 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     2341 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     2401 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     2461 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     2521 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     2581 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     2641 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     2701 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     2761 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     2821 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2881 cgggaaacct gtcgtgccag cggatcccga agttcctatt ctctagaaag tataggaact
     2941 tcaggtctga agaggagttt acgtccagcc aagctagctt ggctgcaggt cgtcgaaatt
     3001 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     3061 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     3121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     3181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     3241 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     3301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     3361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     3421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     3481 ctcttcctca tctccgggcc tttcgacctg cagcctgttg acaattaatc atcggcatag
     3541 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatgaccga gtacaagccc
     3601 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     3661 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     3721 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     3781 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     3841 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     3901 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     3961 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     4021 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     4081 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     4141 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaggggatca attctctaga
     4201 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     4261 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4321 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     4381 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     4441 atggcttctg aggcggaaag aaccagctgg ggctcgacta gagcttgcgg aacccttcga
     4501 agttcctatt ctctagaaag tataggaact tcatcagtca ggtacataat ggatccggaa
     4561 gagggggaag ggattctccc aggcccaggg cggtcctcag aagccaggag gcagcagaga
     4621 actcccagaa aggtattgca acactcccct cccccctccg gagaagggtg cggccttctc
     4681 cccgcctact ccactgcagc tcccttactg ataacaactc agagcgactt tgggagagca
     4741 agtgcttcct gcctccaaaa cagcccaact gagccctcgc tccttccctc cactccccgg
     4801 agtgcgcgat ggaggtctgg ctcagcacgc ccctcttgag gcaactcaag tcggaaacgt
     4861 gcttgcaccc gccccgcagc cgctcagccc tactgcccgt ccccgccccc agcgcgcgct
     4921 tcctgccacg ttgcgcaggg gcgcgcggcc agactctgcg gcgcggggcc gaggggaggg
     4981 ccggaacctg ggagcgcctc ctcgccgccc ccgctggccg gcggatggac tcaacttgca
     5041 cgaacacgag ccaatggcaa gggccagttt tctgggcccc gagagccaat cagacgacga
     5101 ggcccggccg gcggcgggta aaacgactcc cccagaggaa ggggagggtg ggcggccgct
     5161 cgcgcgcgag ctactttcgc tgaccctccc tcccctcccc cgccccgcca gaggccgacc
     5221 gcgcccgcac gtccagctcg cctcacccca cctacctccc gccccaccca gtgggcagag
     5281 cgaggctgcc ggcggctgcg cactccggct gccgttaact ggcggccgcc accgcggtgg
     5341 agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca
     5401 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5461 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     5521 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     5581 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     5641 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5701 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5761 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5821 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5881 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5941 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6001 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6061 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6121 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6181 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6241 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6301 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6361 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6421 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6541 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6601 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6661 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6721 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6781 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6841 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6901 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6961 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7021 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7081 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7141 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7201 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7261 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7321 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7381 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7441 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7501 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     7561 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     7621 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     7681 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     7741 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     7801 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     7861 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     7921 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     7981 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     8041 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     8101 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     8161 gtgaattgta atacgactca ctatagggcg aattgggtac cataaatgga gaaaaaggag
     8221 agaggcattc atgggagtgg aaagttaagc cttaggttgg attctcaata catctattgt
     8281 tgtaaaactc cctggactga gaataggccc aaatgtggaa caccacctga cgggagaggt
     8341 gatagacact gaaaattaag gatcaaggca aaggatcaac aaaaagtgta ctaaggagtt
     8401 ataaaagaac tgcagtgttg aggccccagc tacagcctcg atttgtggtg tatgtaacta
     8461 atctgtctgg tttcatgagt catcagactt ctaagatcag gaaggggaaa atgccaatgc
     8521 tctgtctagg ggttggataa gccagtctaa taaatgaaaa tggggctaaa atgagtgttc
     8581 taaaatacct tttgataagg ctgcagaagg agcgggagaa atggatatga agtactgggc
     8641 tctttaaaaa tgattaaaat tctgcttaca tagtctaact cgcgacactg taatttcata
     8701 ctgtagtaag gatctcaagc aggagagtat aaaactcggg tgagcatgtc tttaatctac
     8761 ctcgatggaa aatactccga ggcggatcac aagcaataat aacctgtagt tttgctgcat
     8821 aaaaccccag atgactacct atcctcccat tttccttatt tgcccctatt aaaaaacttc
     8881 ccgacaaaac cgaaaatctg tgggaagtct tgtccctcca attttacacc tgttcaattc
     8941 ccctgcagga caacgcccac acaccaggtt agcctttaag cctctcgagg tc
//
