LOCUS       sequence-181623-        8466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral overexpression vector for Tau bimolecular fluorescence 
            complementation (BiFC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J, 
            Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L,
            Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY
  TITLE     TRIM28 regulates the nuclear accumulation and toxicity of both 
            alpha-synuclein and tau.
  JOURNAL   Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809.
  PUBMED    27779468
REFERENCE   2  (bases 1 to 8466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-10-25"; volume: "5. pii: e19809. doi"; pages: " 
            10.7554/eLife.19809"
COMMENT     Sequence Label: pBABE-Tau-nYFP
FEATURES             Location/Qualifiers
     source          1..8466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             5..474
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    539..738
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             939..1355
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1285..1307
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1323..1339
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(1392..1411)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1583..1962
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1963..2166
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2117..2137
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2163..2187
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2397..2415
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2397..2414
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2428..2447
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     protein_bind    2493..2517
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3877..3901)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3963..3965,3966..4424)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(4005..4026)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4266..4285)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        4457..4944
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4492..4515)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4877..4896
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4958..5761
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5021..5040)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5631..5650
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5957..6426
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6556..6573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6727..7315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6807..6826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7475..8266,8267..8335))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8098..8117
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8336..8440)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cagcctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
       61 ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
      121 tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt
      181 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
      241 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
      301 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
      361 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
      421 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg
      481 ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggcaa
      541 gctggccagc aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc
      601 gcctgcgtcg gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg
      661 acgagttctg aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt
      721 tttgtggccc gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg
      781 ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg
      841 tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc
      901 tctgtctgac tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct
      961 taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag
     1021 atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg
     1081 gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct
     1141 tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct
     1201 tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc
     1261 ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct
     1321 ccctttatcc agccctcact ccttctctag gcgccggccg gatctcccga tcccctatgg
     1381 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     1441 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     1501 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     1561 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     1621 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     1681 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     1741 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     1801 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     1861 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     1921 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     1981 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2041 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2101 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2161 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     2221 catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat tggaacgcgg
     2281 attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc cacccccttg
     2341 gcttcgttag aacgcggcta caattaatac ataaccttat gtatcataca catacgattt
     2401 aggtgacact atagaataac atccactttg cctttctctc cacaggtgtc cactcccagg
     2461 tccaactgca cctcggttct atcgattgat caacaagttt gtacaaaaaa gcaggctccg
     2521 cggccgcccc cttcaccatg gctgagcccc gccaggagtt cgaagtgatg gaagatcacg
     2581 ctgggacgta cgggttgggg gacaggaaag atcagggggg ctacaccatg caccaagacc
     2641 aagagggtga cacggacgct ggcctgaaag aatctcccct gcagaccccc actgaggacg
     2701 gatctgagga accgggctct gaaacctctg atgctaagag cactccaaca gcggaagatg
     2761 tgacagcacc cttagtggat gagggagctc ccggcaagca ggctgccgcg cagccccaca
     2821 cggagatccc agaaggaacc acagctgaag aagcaggcat tggagacacc cccagcctgg
     2881 aagacgaagc tgctggtcac gtgacccaag ctcgcatggt cagtaaaagc aaagacggga
     2941 ctggaagcga tgacaaaaaa gccaaggggg ctgatggtaa aacgaagatc gccacaccgc
     3001 ggggagcagc ccctccaggc cagaagggcc aggccaacgc caccaggatt ccagcaaaaa
     3061 ccccgcccgc tccaaagaca ccacccagct ctggtgaacc tccaaaatca ggggatcgca
     3121 gcggctacag cagccccggc tccccaggca ctcccggcag ccgctcccgc accccgtccc
     3181 ttccaacccc acccacccgg gagcccaaga aggtggcagt ggtccgtact ccacccaagt
     3241 cgccgtcttc cgccaagagc cgcctgcaga cagcccccgt gcccatgcca gacctgaaga
     3301 atgtcaagtc caagatcggc tccactgaga acctgaagca ccagccggga ggcgggaagg
     3361 tgcagataat taataagaag ctggatctta gcaacgtcca gtccaagtgt ggctcaaagg
     3421 ataatatcaa acacgtcccg ggaggcggca gtgtgcaaat agtctacaaa ccagttgacc
     3481 tgagcaaggt gacctccaag tgtggctcat taggcaacat ccatcataaa ccaggaggtg
     3541 gccaggtgga agtaaaatct gagaagcttg acttcaagga cagagtccag tcgaagattg
     3601 ggtccctgga caatatcacc cacgtccctg gcggaggaaa taaaaagatt gaaacccaca
     3661 agctgacctt ccgcgagaac gccaaagcca agacagacca cggggcggag atcgtgtaca
     3721 agtcgccagt ggtgtctggg gacacgtctc cacggcatct cagcaatgtc tcctccaccg
     3781 gcagcatcga catggtagac tcgccccagc tcgccacgct agctgacgag gtgtctgcct
     3841 ccctggccaa gcagggtttg aagggtgggc gcgccgaccc agctttcttg tacaaagtgg
     3901 ttgatccccg gggatcctct agagtcgacg gcggaggagg ttcaggaggc ggaggcgcgc
     3961 cagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     4021 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     4081 gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4141 tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
     4201 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4261 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4321 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4381 agctggagta caactacaac agccacaacg tctatatcac tgcctagtta acaacctcga
     4441 gagatctaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     4501 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     4561 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     4621 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     4681 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     4741 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     4801 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcggggcggg cgcccgaagg
     4861 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     4921 tcttcctcat ctccgggcct ttcgacctgc agccaatatg ggatcggcca ttgaacaaga
     4981 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     5041 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     5101 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     5161 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     5221 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     5281 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     5341 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     5401 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5461 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg atgatctcgt
     5521 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5581 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5641 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5701 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5761 aggggatccg tcgacctgca gccaagctta tcgataaaat aaaagatttt atttagtctc
     5821 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     5881 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca
     5941 ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     6001 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     6061 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc
     6121 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     6181 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     6241 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
     6301 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     6361 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     6421 ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa
     6481 atggccatag ttgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6541 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6601 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6661 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6721 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6781 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6841 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6901 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6961 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7021 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7081 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7141 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7201 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7261 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7321 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7381 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgcggc cgcaaatcaa
     7441 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7501 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7561 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7621 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7681 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7741 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7801 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7861 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7921 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7981 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8041 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8101 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8161 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8221 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8281 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8341 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8401 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8461 cacctg
//
