LOCUS       sequence-181627-        7730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral overexpression vector for alpha-Syn/Tau bimolecular 
            fluorescence complementation (BiFC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J, 
            Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L,
            Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY
  TITLE     TRIM28 regulates the nuclear accumulation and toxicity of both 
            alpha-synuclein and tau.
  JOURNAL   Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809.
  PUBMED    27779468
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-10-25"; volume: "5. pii: e19809. doi"; pages: " 
            10.7554/eLife.19809"
COMMENT     Sequence Label: pBABE-Tau-cYFP
FEATURES             Location/Qualifiers
     source          1..7730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(66..395)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      80..215
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     380..400
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(436..687)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(478..499)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    752..776
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2136..2160)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2206..2225)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(2238..2256)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2239..2256)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2466..2490)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(2487..2690)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2516..2536)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2691..3070
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3242..3261
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(3298..3714)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(3314..3330)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3346..3368)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3915..4114
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             4179..4648
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        4679..4783
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4784..4852,4853..5644)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5002..5021)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5804..6392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6293..6312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6546..6563
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             6693..7162
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7466..7486)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc ggtcatggta
       61 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
      121 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
      181 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
      241 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
      301 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
      361 gagcctgggg actttccaca ccctaactga cacacattcc acagggtcga ccactgtgct
      421 ggcgaatagc ttctacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
      481 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      541 gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      601 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      661 caccttgatg ccgttcttct gcttgtcctg cagcgaacct ccgcctcctg aacctcctcc
      721 gccgtcgact ctagaggatc cccggggatc aaccactttg tacaagaaag ctgggtcggc
      781 gcgcccaccc ttcaaaccct gcttggccag ggaggcagac acctcgtcag ctagcgtggc
      841 gagctggggc gagtctacca tgtcgatgct gccggtggag gagacattgc tgagatgccg
      901 tggagacgtg tccccagaca ccactggcga cttgtacacg atctccgccc cgtggtctgt
      961 cttggctttg gcgttctcgc ggaaggtcag cttgtgggtt tcaatctttt tatttcctcc
     1021 gccagggacg tgggtgatat tgtccaggga cccaatcttc gactggactc tgtccttgaa
     1081 gtcaagcttc tcagatttta cttccacctg gccacctcct ggtttatgat ggatgttgcc
     1141 taatgagcca cacttggagg tcaccttgct caggtcaact ggtttgtaga ctatttgcac
     1201 actgccgcct cccgggacgt gtttgatatt atcctttgag ccacacttgg actggacgtt
     1261 gctaagatcc agcttcttat taattatctg caccttcccg cctcccggct ggtgcttcag
     1321 gttctcagtg gagccgatct tggacttgac attcttcagg tctggcatgg gcacgggggc
     1381 tgtctgcagg cggctcttgg cggaagacgg cgacttgggt ggagtacgga ccactgccac
     1441 cttcttgggc tcccgggtgg gtggggttgg aagggacggg gtgcgggagc ggctgccggg
     1501 agtgcctggg gagccggggc tgctgtagcc gctgcgatcc cctgattttg gaggttcacc
     1561 agagctgggt ggtgtctttg gagcgggcgg ggtttttgct ggaatcctgg tggcgttggc
     1621 ctggcccttc tggcctggag gggctgctcc ccgcggtgtg gcgatcttcg ttttaccatc
     1681 agcccccttg gcttttttgt catcgcttcc agtcccgtct ttgcttttac tgaccatgcg
     1741 agcttgggtc acgtgaccag cagcttcgtc ttccaggctg ggggtgtctc caatgcctgc
     1801 ttcttcagct gtggttcctt ctgggatctc cgtgtggggc tgcgcggcag cctgcttgcc
     1861 gggagctccc tcatccacta agggtgctgt cacatcttcc gctgttggag tgctcttagc
     1921 atcagaggtt tcagagcccg gttcctcaga tccgtcctca gtgggggtct gcaggggaga
     1981 ttctttcagg ccagcgtccg tgtcaccctc ttggtcttgg tgcatggtgt agcccccctg
     2041 atctttcctg tcccccaacc cgtacgtccc agcgtgatct tccatcactt cgaactcctg
     2101 gcggggctca gccatggtga agggggcggc cgcggagcct gcttttttgt acaaacttgt
     2161 tgatcaatcg atagaaccga ggtgcagttg gacctgggag tggacacctg tggagagaaa
     2221 ggcaaagtgg atgttattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     2281 atgtattaat tgtagccgcg ttctaacgaa gccaaggggg tgggcctata gactctatag
     2341 gcggtactta cgtcactctt ggcacgggga atccgcgttc caatgcaccg ttcccggccg
     2401 cggaggctgg atcggtcccg gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc
     2461 caggcgatct gacggttcac taaacgagct ctgcttatat agacctccca ccgtacacgc
     2521 ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga
     2581 ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
     2641 gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata
     2701 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
     2761 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
     2821 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
     2881 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
     2941 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
     3001 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat
     3061 aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc
     3121 ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc ttaaattttg
     3181 ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg gcttaactat
     3241 gcggcatcag agcagattgt actgagagtg caccataggg gatcgggaga tccggccggc
     3301 gcctagagaa ggagtgaggg ctggataaag ggaggatcga ggcggggtcg aacgaggagg
     3361 ttcaaggggg agagacgggg cggatggagg aagaggaggc ggaggcttag ggtgtacaaa
     3421 gggcttgacc cagggagggg ggtcaaaagc caaggcttcc caggtcacga tgtaggggac
     3481 ctggtctggg tgtccatgcg ggccaggtga aaagaccttg atcttaacct gggtgatgag
     3541 gtctcggtta aaggtgccgt ctcgcggcca tccgacgtta aaggttggcc attctgcaga
     3601 gcagaaggta acccaacgtc tcttcttgac atctaccgac tggttgtgag cgatccgctc
     3661 gacatctttc cagtgaccta aggtcaaact taagggagtg gtaacagtct ggccctaatt
     3721 ttcagacaaa tacagaaaca cagtcagaca gagacaacac agaacgatgc tgcagcgctg
     3781 cagcagacaa gacgcgcggc ttcggttcca aaccgaaagc aaaaattcag acggaggcgg
     3841 gaactgtttt aggttctcgt ctcctaccag aaccacatat cctgacgggg tcggattcca
     3901 catcgactcc cttcctcagg tcgggccaca aaaacggccc ccaaagtccc tgggacgtct
     3961 cccagggttg cggccgggtg ttcagaactc gtcagttcca ccacgggtcc gccagataca
     4021 gagctagtta gctaactagt accgacgcag gcgcataaaa tcagtcatag acactagaca
     4081 atcggacaga cacagataag ttgctggcca gcttgcctcc cggtggtggg tcggtggtcc
     4141 ctgggcaggg gtctcccgat cccggacgag cccccaaatg aaagaccccc gctgacgggt
     4201 agtcaatcac tcagaggaga ccctcccaag gaacagcgag accacaagtc ggatgcaact
     4261 gcaagagggt ttattggata cacgggtacc cgggcgactc agtcaatcgg aggactggcg
     4321 ccccgagtga ggggttgtgg gctcttttat tgagctcggg gagcagaagc gcgcgaacag
     4381 aagcgagaag cgaactgatt ggttagttca aataaggcac agggtcattt caggtccttg
     4441 gggcaccctg gaaacatctg atggttctct agaaactgct gagggctgga ccgcatctgg
     4501 ggaccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga tatcctgttt
     4561 ggcccatatt cagctgttcc atctgttctt ggccctgagc cggggcagga actgcttacc
     4621 acagatatcc tgtttggccc atattcaggc tgcaggtggc acttttcggg gaaatgtgcg
     4681 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4741 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4801 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4861 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4921 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4981 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5041 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5101 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5161 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5221 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5281 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5341 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5401 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5461 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5521 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5581 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5641 gtaactgtca gaccaagttt actcatatat actttagatt gatttgcggc cgcaaaagga
     5701 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5761 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5821 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5881 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5941 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6001 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6061 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6121 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6181 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6241 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6301 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6361 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6421 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6481 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6541 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6601 ccgcgcgttg gccgattcat taatgcaact atggccattt aatgtaaata cttaagaaaa
     6661 aaaaccaaat taattttgat acatgctgca tgtgaaagac ccccgctgac gggtagtcaa
     6721 tcactcagag gagaccctcc caaggaacag cgagaccaca agtcggatgc aactgcaaga
     6781 gggtttattg gatacacggg tacccgggcg actcagtcaa tcggaggact ggcgccccga
     6841 gtgaggggtt gtgggctctt ttattgagct cggggagcag aagcgcgcga acagaagcga
     6901 gaagcgaact gattggttag ttcaaataag gcacagggtc atttcaggtc cttggggcac
     6961 cctggaaaca tctgatggtt ctctagaaac tgctgagggc tggaccgcat ctggggacca
     7021 tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct gtttggccca
     7081 tattcagctg ttccatctgt tcttggccct gagccggggc aggaactgct taccacagat
     7141 atcctgtttg gcccatattc agctgttcca tctgttcctg accttgatct gaacttctct
     7201 attctcagtt atgtattttt ccatgccttg caaaatggcg ttacttaagc tagcttgcca
     7261 aacctacagg tggggtcttt cattcccccc tttttctgga gactaaataa aatcttttat
     7321 tttatcgatg catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg
     7381 cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
     7441 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
     7501 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
     7561 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg
     7621 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
     7681 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc
//
