LOCUS       sequence-181644-        4382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of a fluorescent protein, mini Singlet Oxygen 
            Generator (miniSOG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: pBAD miniSOG
FEATURES             Location/Qualifiers
     source          1..4382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..300
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     223..242
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             327..349
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             359..676
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     CDS             725..742
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(798..815)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      968..1054
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1146..1173
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1192..1283
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1284..1352,1353..2144)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1502..1521)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2315..2903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2804..2823
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3057..3074
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3089..3229
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3155..3174)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3315..3337
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3493..4371)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
       61 tcttttactg gctgttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
      121 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
      181 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
      241 ccataagatt agccgatcct acctgacgct ttttatcgca actctctact gtttctcgat
      301 acccgttttt ttgggctaga aataattttg tttaacttta agaaggagat atggatccat
      361 ggaaaaaagc ttcgtgatta ctgacccgcg cctgccggat aatccgatca tcttcgcctc
      421 cgatggcttc ctggaactga ctgaatacag ccgtgaggaa attctgggtc gtaatggccg
      481 tttcctgcag ggcccggaaa ccgatcaagc aactgttcag aaaattcgtg atgcaatccg
      541 tgatcaacgt gaaatcaccg tgcagctgat taactatacc aaaagcggca agaaattctg
      601 gaacctgttg cacctgcagc cgatgcgcga tcagaaaggt gagctgcagt acttcatcgg
      661 ggttcagctg gatggtgaat tcatccctaa ccctctcctc ggtctcgatt ctacgcgtac
      721 cggtcatcat caccatcacc attgagttta aacggtctcc agcttggctg ttttggcgga
      781 tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa
      841 cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa
      901 gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc
      961 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1021 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc
     1081 gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat
     1141 taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat
     1201 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1261 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1321 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1381 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1441 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1501 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     1561 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1621 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1681 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1741 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1801 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     1861 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1921 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1981 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2041 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2101 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2161 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2221 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2281 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2341 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2401 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2461 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2521 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2581 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2641 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2701 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2761 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2821 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2881 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2941 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3001 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3061 agtcagtgag cgaggaagcg gtagagcgcc tgatgcggta ttttctcctt acgcatctgt
     3121 gcggtatttc acaccgtata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3181 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     3241 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     3301 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     3361 cgcgaggcag cagatcaatt cgcgcgcgaa ggcgaagcgg catgcataat gtgcctgtca
     3421 aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg
     3481 attcgttacc aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc
     3541 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg
     3601 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag
     3661 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg
     3721 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc
     3781 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg
     3841 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg
     3901 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat
     3961 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc
     4021 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa
     4081 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc
     4141 tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt
     4201 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg
     4261 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg
     4321 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat
     4381 ac
//
