LOCUS       sequence-181646-        5729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of a fluorescent protein, Infrared Fluorescent 
            Protein 1.4 + Heme oxygenase-1 (IFP1.4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Shu X, Royant A, Lin MZ, Aguilera TA, Lev-Ram V, Steinbach PA, Tsien
            RY
  TITLE     Mammalian expression of infrared fluorescent proteins engineered 
            from a bacterial phytochrome.
  JOURNAL   Science. 2009 May 8;324(5928):804-7. doi: 10.1126/science.1168683.
  PUBMED    19423828
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2009-05-8- 8"; volume: "324"; issue: 
            "5928"; pages: "804-7"
COMMENT     Sequence Label: pBAD IFP1.4
FEATURES             Location/Qualifiers
     source          1..5729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..303
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     226..245
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             330..352
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1331..1348
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2148..2165)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2318..2404
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2496..2523
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2542..2633
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2634..2702,2703..3494)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2852..2871)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3665..4253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4154..4173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4407..4424
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4439..4579
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4505..4524)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4665..4687
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4843..5721)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact
       61 gcgtctttta ctggctgttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc
      121 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg
      181 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt
      241 tatccataag attagccgat cctacctgac gctttttatc gcaactctct actgtttctc
      301 gatacccgtt tttttgggct agaaataatt ttgtttaact ttaagaagga gatatggatc
      361 catggctcgt gatccgctgc cgtttttccc gcccctgtat ctgggtggtc cggagatcac
      421 cactgaaaac tgtgagcgtg aaccgattca tatcccgggt tccatccagc cgcacggtgc
      481 actgctgacc gcagatggcc actctggcga agtgctgcag gtgtctctga acgctgcaac
      541 tttcctgggt caggagccga ccgttctgcg tggccagacc ctggcggctc tgctgccgga
      601 acagtggccg gctctgcagg cggctctgcc gccgggttgc ccggatgcgc tgcagtaccg
      661 cgctaccctg gattggccgg cagctggtca cctgtccctg accgttcacc gtgtggctga
      721 actgttgatc ctggaatttg aaccgaccga agcatgggat tccatcggcc cgcacgcact
      781 gcgtaacgct atgtttgcac tggaatccgc accgaacctg cgtgctctgg cggaggttgc
      841 tactcagacc gttcgcgaac tgactggttt tgatcgcgta atgctgtaca agttcgcacc
      901 ggatgcgact ggtgaaatga tcgcggaagc tcgtcgcgaa ggtatgcaag cttttctggg
      961 tcaccgtttc ccggctagcc acaccccggc gcaggctcgc gcactgtaca ctcgtcacct
     1021 gctgcgtctg actgcggaca cccgcgcagc tgcggttccg ctggacccgg ttctgaaccc
     1081 gcagaccaac gcgccgaccc cgctgggcgg cgcggttctg cgtgcgacca gcccgatgca
     1141 tatgcagtac ctgcgtaaca tgggtgttgg cagctccctg tctgtgagcg tggtagttgg
     1201 cggtcagctg tggggtctga tcgtgtgtca ccaccagact ccgtacgttc tgccgccgga
     1261 tctgcgcacc accctggagg agctgggtcg taagctgagc ggccaggtac agcgcaaaga
     1321 ggcggaattc catcatcacc atcaccattg aaagaaggag atatacatac ccatgagtgt
     1381 caacttagct tcccagttgc gggaagggac gaaaaaatcc cactccatgg cggagaacgt
     1441 cggctttgtc aaatgcttcc tcaagggcgt tgtcgagaaa aattcctacc gtaagctggt
     1501 tggcaatctc tactttgtct acagtgccat ggaagaggaa atggcaaaat ttaaggacca
     1561 tcccatcctc agccacattt acttccccga actcaaccgc aaacaaagcc tagagcaaga
     1621 cctgcaattc tattacggct ccaactggcg gcaagaagtg aaaatttctg ccgctggcca
     1681 agcctatgtg gaccgagtcc ggcaagtggc cgctacggcc cctgaattgt tggtggccca
     1741 ttcctacacc cgttacctgg gggatctttc cggcggtcaa attctcaaga aaattgccca
     1801 aaatgccatg aatctccacg atggtggcac agctttctat gaatttgccg acattgatga
     1861 cgaaaaggct tttaaaaata cctaccgtca agctatgaat gatctgccca ttgaccaagc
     1921 caccgccgaa cggattgtgg atgaagccaa tgacgccttt gccatgaaca tgaaaatgtt
     1981 caacgaactt gaaggcaacc tgatcaaggc gatcggcatt atggtgttca acagcctcac
     2041 ccgtcgccgc agtcaaggca gcaccgaagt tggcctcgcc acctccgaag gctaggttta
     2101 aacggtctcc agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt
     2161 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg
     2221 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     2281 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     2341 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     2401 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
     2461 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
     2521 ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc
     2581 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     2641 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2701 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2761 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2821 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     2881 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2941 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3001 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3061 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3121 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3181 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3241 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3301 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3361 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3421 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3481 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3541 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3601 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3661 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3721 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3781 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3841 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3901 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3961 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4021 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4081 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4141 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4201 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4261 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4321 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4381 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gtagagcgcc
     4441 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgtata tggtgcactc
     4501 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     4561 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     4621 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     4681 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa
     4741 ggcgaagcgg catgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct
     4801 atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc
     4861 tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca
     4921 ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt
     4981 ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc
     5041 gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg
     5101 cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc
     5161 gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt
     5221 aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga
     5281 atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg
     5341 ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag
     5401 ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc
     5461 ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg
     5521 tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt
     5581 gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc
     5641 ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg
     5701 atcattttgc gcttcagcca tacttttca
//
