LOCUS       sequence-181650-        9634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of humanized Mb3Cpf1 and crRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9634)
  TITLE     Zhang lab Cpf1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pY129 (pcDNA3.1-U6-huMb3Cpf1)
FEATURES             Location/Qualifiers
     source          1..9634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(58..78)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        63..392
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      243..378
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     305..324
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             459..1253
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(513..532)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1123..1142
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1427..1548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1464..1483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1518..1537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1597..1613)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1597..1613)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1610..1632)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1621..1637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1645..1651,1652..1669,1670..1675))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1690..1711
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1828..1845)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1999..2587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2079..2098)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2758..3549,3550..3618))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3381..3400
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3619..3723)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3798..3817)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3921..4169
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3921..3941
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4092..4111
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        4246..4625
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4626..4829
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4780..4800
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4874..4893
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4874..4892
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4934..4943
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4946..4966
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8771..8818
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8825..8905
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(8979..8996)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8985..9209
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9255..9634,1..49)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9342..9361)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9552..9573
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
       61 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
      121 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
      181 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
      241 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
      301 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
      361 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
      421 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
      481 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
      541 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
      601 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
      661 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
      721 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
      781 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
      841 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
      901 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
      961 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     1021 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     1081 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     1141 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     1201 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     1261 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     1321 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     1381 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     1441 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1501 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     1561 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1621 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1681 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1741 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1801 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1861 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1921 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1981 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2041 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2101 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2161 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2221 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2341 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2401 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2461 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2521 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2581 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2641 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2701 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2761 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2821 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2881 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2941 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3001 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3061 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3121 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3181 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3241 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3301 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3361 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3421 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3481 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3541 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3601 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3661 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3721 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     3781 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     3841 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     3901 caaggcttga ccgacaattg gagggcctat ttcccatgat tccttcatat ttgcatatac
     3961 gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag
     4021 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     4081 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
     4141 tatatatctt gtggaaagga cgaaacaccg aatttctact gtttgtagat ggagacgata
     4201 tatcgtctct tttttttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     4261 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     4321 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     4381 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     4441 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     4501 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4561 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4621 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     4681 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     4741 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     4801 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
     4861 ggcttatcga aattaatacg actcactata gggagaccca agctggctag cgtttaaact
     4921 taagcttacc ggtgccacca tggccccaaa gaagaagcgg aaggtcggta tccacggagt
     4981 cccagcagcc ctgttccagg acttcaccca cctgtacccc ctgagcaaga ccgtcagatt
     5041 cgagctgaag cccatcggca agaccctgga acacatccac gccaagaact ttctgaacca
     5101 ggacgagaca atggccgaca tgtaccagaa agtgaaggcc atcctggacg actaccaccg
     5161 ggactttatc gccgacatga tgggcgaagt gaagctgacc aagctggccg agttctacga
     5221 cgtgtacctg aagttccgga agaaccccaa ggacgacggc ctgcagaagc agctgaaaga
     5281 cctgcaggct gtgctgcgga aagaaatcgt gaagcctatc ggaaacggcg gcaagtacaa
     5341 ggccggctac gacagactgt tcggcgccaa gctgttcaag gacggcaaag agctgggcga
     5401 cctggccaag ttcgtgatcg cccaggaagg cgagagcagc cctaagctgg cccacctggc
     5461 ccatttcgag aagttcagca cctacttcac cggcttccac gacaaccgca agaacatgta
     5521 cagcgacgag gacaagcaca ccgccattgc ctaccggctg atccacgaga acctgccccg
     5581 gttcatcgac aacctgcaga tcctggccac catcaagcag aagcacagcg ccctgtacga
     5641 ccagatcatc aacgagctga ccgccagcgg cctggatgtg tctctggcct ctcacctgga
     5701 tggctaccac aagctgctga cacaggaagg catcaccgcc tacaacaccc tgctgggcgg
     5761 aatctctggc gaggccggca gcagaaagat ccagggcatc aatgaactga tcaacagcca
     5821 ccacaaccag cactgccaca agagcgagcg gattgccaag ctgcggcccc tgcacaagca
     5881 gattctgagc gacggaatgg gcgtgtcctt cctgcccagc aagttcgccg acgacagcga
     5941 agtgtgccag gccgtgaatg agttctaccg gcactacgcc gacgtgttcg ccaaggtgca
     6001 gagcctgttc gacggcttcg acgactatca gaaagacggc atctacgtgg agtacaagaa
     6061 cctgaacgag ctgtccaagc aggccttcgg cgacttcgct ctgctgggaa gagtgctgga
     6121 cggctactat gtggacgtcg tgaaccccga gttcaacgag agatttgcca aggccaagac
     6181 cgacaacgcc aaagccaagc tgacaaaaga gaaggacaag ttcatcaagg gcgtgcactc
     6241 cctggcttct ctggaacagg ccatcgagca ctacaccgcc agacacgacg acgagtctgt
     6301 gcaggccgga aagctgggcc agtacttcaa gcacggactg gccggcgtgg acaaccccat
     6361 ccagaagatc cacaacaacc actctacaat caagggcttc ctggaaagag agcggcctgc
     6421 cggcgaaaga gccctgccca agatcaagag cgacaagagc cccgagatca gacagctgaa
     6481 agagctgctg gacaacgccc tgaacgtggc ccacttcgcc aaactgctga ccaccaagac
     6541 cacactgcac aatcaggatg gcaacttcta cggcgagttc ggagccctgt atgatgagct
     6601 ggccaagatc gccaccctgt acaacaaagt gcgggactac ctgagccaga agcccttcag
     6661 caccgagaag tacaagctga atttcggcaa ccctaccctg ctgaacggct gggacctgaa
     6721 caaagagaaa gataacttcg gcgtgatcct gcagaaggac ggctgctact acctggccct
     6781 gctggataag gcccacaaga aggtgttcga taacgccccc aacaccggca agtctgtgta
     6841 tcagaagatg atctataagc tgctgcctgg ccccaacaag atgctgccca aggtgttctt
     6901 cgccaagagc aacctggact actacaaccc cagcgccgaa ctgctggaca aatacgccca
     6961 gggcacacac aagaaaggcg acaacttcaa cctgaaggac tgccacgccc tgatcgattt
     7021 cttcaaggcc ggaatcaaca agcaccccga gtggcagcac ttcggcttca agttcagccc
     7081 caccagcagc taccaggacc tgagcgactt ctacagagag gtggaacccc agggctacca
     7141 agtgaagttc gtggacatca acgccgacta catcaatgag ctggtggaac agggccagct
     7201 gtacctgttt cagatctaca acaaggactt tagccccaag gcccacggca agcccaacct
     7261 gcacaccctg tatttcaagg ccctgtttag cgaggacaac ctcgtgaatc ccatctacaa
     7321 actgaacggg gaggccgaga tcttctaccg gaaggcctcc ctggacatga acgagacaac
     7381 catccacaga gccggcgagg tgctggaaaa caagaaccct gacaacccta agaaacggca
     7441 gttcgtgtac gacatcatca aggacaagcg gtacacccag gataagttca tgctgcacgt
     7501 gcccatcacc atgaacttcg gagtgcaggg catgaccatc aaagagttca acaaaaaagt
     7561 gaaccagagc atccagcagt acgacgaagt gaacgtgatc ggcatcgacc ggggcgagcg
     7621 gcatctgctg tatctgaccg tgatcaactc caagggcgag attctggaac agagatccct
     7681 gaacgacatc accaccgcct ccgccaacgg cacccagatg accacccctt accacaagat
     7741 cctggacaag cgcgagatcg agcggctgaa cgccagagtg ggatggggag agatcgagac
     7801 aatcaaagaa ctgaagtccg gctacctgtc ccacgtggtg catcagatct cccagctgat
     7861 gctgaagtac aacgccatcg tggtgctgga agatctgaat tttggcttca agaggggccg
     7921 gttcaaggtg gaaaagcaga tctaccagaa cttcgagaat gccctgatca agaaactgaa
     7981 ccacctggtg ctgaaagaca aggccgacga cgagatcggc agctacaaga acgccctgca
     8041 gctgactaac aacttcaccg atctgaagag tatcggcaag cagaccggct ttctgttcta
     8101 cgtgcccgcc tggaatacca gcaagatcga ccccgagaca ggcttcgtgg acctgctgaa
     8161 gcctagatac gagaatatcg cccagagcca ggccttcttc ggcaagttcg acaagatctg
     8221 ctacaacgcc gataggggct acttcgagtt ccacatcgac tacgccaagt tcaatgacaa
     8281 ggccaaaaac agccggcaga tctggaaaat ctgcagccac ggcgataagc gctacgtgta
     8341 cgataagacc gccaaccaga acaagggcgc caccatcgga gtgaatgtga acgatgagct
     8401 gaagtccctg ttcacccgct accacatcaa cgataagcag cccaatctcg tgatggacat
     8461 ctgccagaac aacgacaaag agtttcacaa gagcctgatg tatctgctga aaacactgct
     8521 ggctctgcgg tacagcaacg ccagctccga cgaggatttc atcctgagcc ccgtggccaa
     8581 cgacgagggc gtgttcttca atagcgccct ggccgacgat acccagcccc agaatgccga
     8641 tgccaacggc gcctaccaca ttgccctgaa gggactgtgg ctgctgaacg aactgaagaa
     8701 cagcgacgat ctgaacaaag tgaagctggc catcgacaac cagacctggc tgaatttcgc
     8761 ccagaacaga aagaggccgg cggccacgaa gaaggccggc caggccaaga agaagaaggg
     8821 atcctaccca tacgatgttc cagattacgc ttatccctac gacgtgcctg attatgcata
     8881 cccatatgat gtccccgact atgcctaaga attctgcaga tatccagcac agtggcggcc
     8941 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     9001 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     9061 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     9121 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     9181 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     9241 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     9301 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     9361 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     9421 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     9481 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     9541 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     9601 ttcttttgat ttataaggga ttttgccgat ttcg
//
