LOCUS       sequence-181652-        9604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of humanized Mb2Cpf1 and crRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9604)
  TITLE     Zhang lab Cpf1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pY128 (pcDNA3.1-U6-huMb2Cpf1)
FEATURES             Location/Qualifiers
     source          1..9604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..43)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        28..357
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      208..343
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     270..289
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             424..1218
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(478..497)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1088..1107
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1392..1513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1429..1448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1483..1502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1562..1578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1562..1578)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1575..1597)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1586..1602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1610..1616,1617..1634,1635..1640))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1655..1676
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1793..1810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1964..2552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2044..2063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2723..3514,3515..3583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3346..3365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3584..3688)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3763..3782)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3886..4134
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3886..3906
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4057..4076
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        4211..4590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4591..4794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4745..4765
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4839..4858
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4839..4857
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4899..4908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4911..4931
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8706..8753
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8760..8840
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(8914..8931)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8920..9144
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9190..9604,1..14)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9277..9296)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9487..9508
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
       61 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
      121 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      181 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      241 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
      301 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      361 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
      421 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
      481 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
      541 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
      601 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
      661 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
      721 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
      781 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
      841 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
      901 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
      961 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     1021 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     1081 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     1141 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     1201 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     1261 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     1321 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     1381 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1441 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1501 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     1561 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1621 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1681 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1741 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1801 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1861 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1921 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1981 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2041 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2101 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2161 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2221 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2281 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2341 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2401 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2461 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2521 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2581 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2641 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2701 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2761 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2821 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2881 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2941 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3001 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3061 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3121 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3181 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3241 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3301 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3361 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3421 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3481 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3541 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3601 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3661 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     3721 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     3781 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     3841 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattggaggg cctatttccc
     3901 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
     3961 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     4021 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     4081 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgaattt
     4141 ctactgtttg tagatggaga cgatatatcg tctctttttt ttcgcgatgt acgggccaga
     4201 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     4261 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     4321 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     4381 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     4441 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     4501 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     4561 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     4621 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     4681 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     4741 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
     4801 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
     4861 acccaagctg gctagcgttt aaacttaagc ttaccggtgc caccatggcc ccaaagaaga
     4921 agcggaaggt cggtatccac ggagtcccag cagccctgtt ccaggacttc acccacctgt
     4981 accccctgag caagaccgtc agattcgagc tgaagcccat cggccggacc ctggaacaca
     5041 tccacgccaa gaactttctg agccaggacg agacaatggc cgacatgtac cagaaagtga
     5101 aagtgatcct ggacgactac caccgggact ttatcgccga catgatgggc gaagtgaagc
     5161 tgaccaagct ggccgagttc tacgacgtgt acctgaagtt ccggaagaac cccaaggacg
     5221 acggcctgca gaagcagctg aaagacctgc aggctgtgct gcggaaagaa agcgtgaagc
     5281 ctatcggaag cggcggcaag tacaagaccg gctacgacag actgttcggc gccaagctgt
     5341 tcaaggacgg caaagagctg ggcgacctgg ccaagttcgt gatcgcccag gaaggcgaga
     5401 gcagccctaa gctggcccac ctggcccatt tcgagaagtt cagcacctac ttcaccggct
     5461 tccacgacaa ccgcaagaac atgtacagcg acgaggacaa gcacaccgcc attgcctacc
     5521 ggctgatcca cgagaacctg ccccggttca tcgacaacct gcagatcctg accaccatca
     5581 agcagaagca cagcgccctg tacgaccaga tcatcaacga gctgaccgcc agcggcctgg
     5641 atgtgtctct ggcctctcac ctggatggct accacaagct gctgacacag gaaggcatca
     5701 ccgcctacaa ccggatcatc ggagaagtga acggctacac caacaagcac aaccagatct
     5761 gccacaagag cgagcggatt gccaagctgc ggcccctgca caagcagatt ctgagcgacg
     5821 gaatgggcgt gtccttcctg cccagcaagt tcgccgacga cagcgagatg tgccaggccg
     5881 tgaatgagtt ctaccggcac tacaccgacg tgttcgccaa ggtgcagagc ctgttcgacg
     5941 gcttcgacga ccaccagaaa gacggcatct acgtggaaca caagaacctg aacgagctgt
     6001 ccaagcaggc cttcggcgac tttgccctgc tgggaagagt gctggacggc tactatgtgg
     6061 acgtcgtgaa ccccgagttc aacgagagat ttgccaaggc caagaccgac aacgccaaag
     6121 ccaagctgac aaaagagaag gacaagttca tcaagggcgt gcactccctg gcttctctgg
     6181 aacaggccat cgagcaccac acagccagac acgacgacga gtctgtgcag gccggaaagc
     6241 tgggccagta cttcaagcac ggactggccg gcgtggacaa ccccatccag aagatccaca
     6301 acaaccactc tacaatcaag ggcttcctgg aaagagagcg gcctgccggc gaaagagccc
     6361 tgcccaagat caagagcggc aagaaccctg agatgaccca gctgagacag ctgaaagagc
     6421 tgctggacaa cgccctgaac gtggcccact tcgccaaact gctgaccaca aagaccaccc
     6481 tggacaacca ggatggcaac ttctacggcg agttcggcgt gctgtacgat gagctggcca
     6541 agatccctac cctgtacaac aaagtgcggg actacctgag ccagaagccc ttcagcaccg
     6601 agaagtacaa gctgaatttc ggcaacccca ccctgctgaa cggctgggac ctgaacaaag
     6661 agaaagataa cttcggcgtg atcctgcaga aagatggctg ctactacctg gctctgctgg
     6721 ataaggccca caagaaggtg ttcgataacg cccccaacac cggcaagaat gtgtatcaga
     6781 aaatggtgta taagctgctg cctggcccca acaagatgct gcccaaggtg ttcttcgcca
     6841 agagcaacct ggactactac aaccccagcg ccgaactgct ggacaaatac gccaagggca
     6901 cacacaagaa aggcgacaac ttcaacctga aggactgcca cgccctgatc gatttcttca
     6961 aggccggcat taacaagcac cccgagtggc agcacttcgg cttcaagttc agccccacca
     7021 gcagctaccg ggacctgagc gacttctaca gagaggtgga accccagggc taccaagtga
     7081 agttcgtgga catcaacgcc gactacatcg acgagctggt ggaacagggc aagctgtacc
     7141 tgtttcagat ctacaacaag gactttagcc ccaaggccca cggcaagccc aacctgcaca
     7201 ccctgtactt taaggccctg ttcagcgagg acaacctggc cgaccccatc tacaaactga
     7261 acggcgaggc ccagatcttc taccggaagg ccagcctgga catgaacgag acaaccatcc
     7321 acagagccgg cgaggtgctg gaaaacaaga atcccgacaa ccctaagaaa cggcagttcg
     7381 tgtacgacat catcaaggac aagcggtaca cccaggataa gttcatgctg cacgtgccca
     7441 tcaccatgaa cttcggagtg cagggcatga ccatcaaaga gttcaacaaa aaagtgaacc
     7501 agagcatcca gcagtacgac gaagtgaatg tgattggcat cgaccggggc gagcggcatc
     7561 tgctgtatct gaccgtgatc aacagcaagg gcgagattct ggaacagaga tccctgaacg
     7621 acatcaccac cgcctccgcc aatggcaccc aagtgaccac cccttaccac aagatcctgg
     7681 ataagcgcga gatcgagcgg ctgaacgcca gagtgggatg gggagagatc gagacaatca
     7741 aagaactgaa gtccggctac ctgtcccacg tggtgcatca gatcaaccag ctgatgctga
     7801 agtacaacgc catcgtggtg ctggaagatc tgaattttgg cttcaagagg ggccggttca
     7861 aggtggaaaa gcagatctac cagaacttcg agaatgccct gatcaagaaa ctgaaccacc
     7921 tggtgctgaa agacaaggcc gacgacgaga tcggcagcta caagaacgcc ctgcagctga
     7981 ctaacaactt caccgatctg aagtccattg gcaagcagac cggctttctg ttctacgtgc
     8041 ccgcctggaa taccagcaag atcgaccccg agacaggctt cgtggacctg ctgaagccta
     8101 gatacgagaa tatcgcccag tcccaggcct tcttcggcaa gttcgacaag atctgctaca
     8161 acaccgacaa gggctacttc gagttccaca tcgactacgc caagttcacc gataaggcca
     8221 aaaacagccg gcagaagtgg gctatctgca gccacggcga caagagatac gtgtacgata
     8281 agaccgccaa ccagaacaag ggcgctgcca agggaatcaa cgtgaacgac gaactgaaat
     8341 ccctgttcgc ccgctaccac atcaacgata agcagcccaa tctcgtgatg gacatctgcc
     8401 agaacaacga caaagagttt cacaagagcc tgatgtgcct gctgaaaacc ctgctggccc
     8461 tgcggtacag caacgccagc tccgacgagg atttcatcct gagccccgtg gccaacgacg
     8521 agggcgtgtt cttcaatagc gccctggccg atgacaccca gccccagaac gctgatgcca
     8581 acggcgccta ccacattgcc ctgaagggac tgtggctgct gaatgagctg aagaactccg
     8641 acgatctgaa caaagtgaag ctggccatcg acaaccagac ctggctgaat ttcgcccaga
     8701 acagaaagag gccggcggcc acgaagaagg ccggccaggc caagaagaag aagggatcct
     8761 acccatacga tgttccagat tacgcttatc cctacgacgt gcctgattat gcatacccat
     8821 atgatgtccc cgactatgcc taagaattct gcagatatcc agcacagtgg cggccgctcg
     8881 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     8941 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     9001 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     9061 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     9121 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     9181 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     9241 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     9301 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     9361 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     9421 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     9481 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     9541 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     9601 aaaa
//
