LOCUS       sequence-181680-       10589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast two hybrid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10589)
  AUTHORS   Gao W, Yang J, Liu W, Wang Y, Shao F
  TITLE     Site-specific phosphorylation and microtubule dynamics control Pyrin
            inflammasome activation.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):E4857-66. doi: 
            10.1073/pnas.1601700113. Epub 2016 Aug 1.
  PUBMED    27482109
REFERENCE   2  (bases 1 to 10589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1601700113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-08-16- 16"; volume: "113"; issue: "33"; pages: 
            "E4857-66"
COMMENT     Sequence Label: pGAD-GH-MEFV
FEATURES             Location/Qualifiers
     source          1..10589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..705
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             751..771
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             787..1128
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     polyA_signal    3560..3694
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3584..3603)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3638..3657
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(3803..3821)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3828..3845)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3828..3844)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3837..3859)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      4247..4434
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4302..4326)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4551..5645)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5646..6051)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(6093..6109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6093..6109)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6106..6128)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6117..6133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6141..6147,6148..6165,6166..6171))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6186..6207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6262..6295
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6373..6406
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6475..6492)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6646..7234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6726..6745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7405..8196,8197..8265))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8028..8047
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8266..8370)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8438..8456
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8494..8516)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8616..8635
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      8652..9816
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca
       61 tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga
      121 gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt
      181 acactgcctc attgatggtg gtacataacg aactaatact gtagccctag acttgatagc
      241 catcatcata tcgaagtttc actacccttt ttccatttgc catctattga agtaataata
      301 ggcgcatgca acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca
      361 tatccgcaat gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca
      421 gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt
      481 tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc
      541 cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac
      601 ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc
      661 tttttctgca caatatttca agctatacca agcatacaat caactccaag ctttgcaaag
      721 atggataaag cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt
      781 accgccgcca attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc
      841 actaacagta gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca
      901 caaccaattg cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt
      961 aaaattgatg atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg
     1021 tataacgcgt ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat
     1081 aactatctat tcgatgatga agatacccca ccaaacccaa aaaaagagat cctagaacta
     1141 gtggatcccc cgggctgcag gaattcaggc cggccaatgg ctaagacccc tagtgaccat
     1201 ctgctgtcca ccctggagga gctggtgccc tatgacttcg agaagttcaa gttcaagctg
     1261 cagaacacca gtgtgcagaa ggagcactcc aggatccccc ggagccagat ccagagagcc
     1321 aggccggtga agatggccac tctgctggtc acctactatg gggaagagta cgccgtgcag
     1381 ctcaccctgc aggtcctgcg ggccatcaac cagcgcctgc tggccgagga gctccacagg
     1441 gcagccattc aggaatattc cacacaagaa aacggcacag atgattccgc agcgtccagc
     1501 tccctggggg agaacaagcc caggagcctg aagactccag accaccccga ggggaacgag
     1561 gggaacggcc ctcggccgta cgggggcgga gctgccagcc tgcggtgcag ccagcccgag
     1621 gccgggaggg ggctgtcgag gaagcccctg agcaaacgca gagagaaggc ctcggagggc
     1681 ctggacgcgc agggcaagcc tcggacccgg agcccggccc tgccgggcgg gagaagcccc
     1741 ggcccctgca gggcgctaga ggggggccag gccgaggtcc ggctgcgcag aaacgccagc
     1801 tccgcgggga ggctgcaggg gctggcgggg ggcgccccgg ggcagaagga gtgcaggccc
     1861 ttcgaagtgt acctgccctc gggaaagatg cgacctagaa gccttgaggt caccatttct
     1921 acaggggaga aggcgcccgc aaatccagaa attctcctga ctctagagga aaagacagct
     1981 gcgaatctgg actcggcaac agaaccccgg gcaaggccca ctccggatgg aggggcatct
     2041 gcggacctga aggaaggccc tggaaatcca gaacattcgg tcaccggaag gccaccagac
     2101 acggctgcga gtccccgttg ccacgcccag gaaggagacc cagttgacgg tacctgtgtg
     2161 cgtgattcct gcagcttccc cgaggcagtt tctgggcacc cccaggcctc aggcagccgc
     2221 tcacctggct gcccccggtg ccaggactcc catgaaagga agagcccggg aagcctaagc
     2281 ccccagcccc tgccacagtg taagcgccac ctgaagcagg tccagctgct cttctgtgag
     2341 gatcacgatg agcccatctg cctcatctgc agtctgagtc aggagcacca aggccaccgg
     2401 gtgcgcccca ttgaggaggt cgccctggaa cacaagaaga aaattcagaa gcagctggag
     2461 catctgaaga agctgagaaa atcaggggag gagcagcgat cctatgggga ggagaaggca
     2521 gtgagctttc tgaaacaaac tgaagcgctg aagcagcggg tgcagaggaa gctggagcag
     2581 gtgtactact tcctggaaca gcaggagcat ttctttgtgg cctcactgga ggacgtgggc
     2641 cagatggttg ggcagatcag gaaggcatat gacacccgcg tatcccagga catcgccctg
     2701 ctcgacgcgc tgattgggga actggaggcc aaggagtgcc agtcagaatg ggaacttctg
     2761 caggacattg gagacatctt gcacagggct aagacagtgc ctgtccctga aaagtggacc
     2821 actcctcaag agataaaaca aaagatccaa ctcctccacc agaagtcaga gtttgtggag
     2881 aagagcacaa agtacttctc agaaaccctg cgttcagaaa tggaaatgtt caatgttccg
     2941 gagctgattg gcgctcaggc acatgctgtt aatgtgattc tggatgcaga aaccgcttac
     3001 cccaacctca tcttctctga tgatctgaag agtgttagac ttggaaacaa gtgggagagg
     3061 ctgcctgatg gcccgcaaag atttgacagc tgtatcattg ttctgggctc tccgagtttc
     3121 ctctctggcc gccgttactg ggaggtggag gttggagaca agacagcatg gatcctggga
     3181 gcctgcaaga catccataag caggaaaggg aacatgactc tgtcgccaga gaatggctac
     3241 tgggtggtga taatgatgaa ggaaaatgag taccaggcgt ccagcgttcc cccgacccgc
     3301 ctgctaataa aggagcctcc caagcgtgtg ggcatcttcg tggactacag agttggaagc
     3361 atctcctttt acaatgtgac agccagatcc cacatctata cattcgccag ctgctctttc
     3421 tctgggcccc ttcaacctat cttcagccct gggacacgtg atggagggaa gaacacagct
     3481 cctctgacta tctgtccagt gggtggtcag gggcctgact gaggcgcgcc tctagaacta
     3541 tagtgagtcg tattacgtag atccagacat gataagatac attgatgagt ttggacaaac
     3601 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     3661 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     3721 gtttcaggtt cagggggagg tgtgggaggt tttttaattc gcggccgcgg cgccaatgca
     3781 ttgggcccgg tacccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
     3841 ttacaacgtc gtgactggga aaaccctgat ctatgaatcg tagatactga aaaaccccgc
     3901 aagttcactt caactgtgca tcgtgcacca tctcaatttc tttcatttat acatcgtttt
     3961 gccttctttt atgtaactat actcctctaa gtttcaatct tggccatgta acctctgatc
     4021 tatagaattt tttaaatgac tagaattaat gcccatcttt tttttggacc taaattcttc
     4081 atgaaaatat attacgaggg cttattcaga agctttggac ttcttcgcca gaggtttggt
     4141 caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga
     4201 aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg
     4261 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
     4321 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
     4381 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggccggtc
     4441 gaaattcccc taccctatga acatattcca ttttgtaatt tcgtgtcgtt tctattatga
     4501 atttcattta taaagtttat gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg
     4561 attttcttaa cttcttcggc gacagcatca ccgacttcgg tggtactgtt ggaaccacct
     4621 aaatcaccag ttctgatacc tgcatccaaa acctttttaa ctgcatcttc aatggcctta
     4681 ccttcttcag gcaagttcaa tgacaatttc aacatcattg cagcagacaa gatagtggcg
     4741 atagggttga ccttattctt tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa
     4801 ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg cagatggcaa caaacccaag
     4861 gaacctggga taacggaggc ttcatcggag atgatatcac caaacatgtt gctggtgatt
     4921 ataataccat ttaggtgggt tgggttctta actaggatca tggcggcaga atcaatcaat
     4981 tgatgttgaa ccttcaatgt aggaaattcg ttcttgatgg tttcctccac agtttttctc
     5041 cataatcttg aagaggccaa aacattagct ttatccaagg accaaatagg caatggtggc
     5101 tcatgttgta gggccatgaa agcggccatt cttgtgattc tttgcacttc tggaacggtg
     5161 tattgttcac tatcccaagc gacaccatca ccatcgtctt cctttctctt accaaagtaa
     5221 atacctccca ctaattctct gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg
     5281 attggagata agtctaaaag agagtcggat gcaaagttac atggtcttaa gttggcgtac
     5341 aattgaagtt ctttacggat ttttagtaaa ccttgttcag gtctaacact acctgtaccc
     5401 catttaggac cacccacagc acctaacaaa acggcatcag ccttcttgga ggcttccagc
     5461 gcctcatctg gaagtggaac acctgtagca tcgatagcag caccaccaat taaatgattt
     5521 tcgaaatcga acttgacatt ggaacgaaca tcagaaatag ctttaagaac cttaatggct
     5581 tcggctgtga tttcttgacc aacgtggtca cctggcaaaa cgacgatctt cttaggggca
     5641 gacattagaa tggtatatcc ttgaaatata tatatatatt gctgaaatgt aaaaggtaag
     5701 aaaagttaga aagtaagacg attgctaacc acctattgga aaaaacaata ggtccttaaa
     5761 taatattgtc aacttcaagt attgtgatgc aagcatttag tcatgaacgc ttctctattc
     5821 tatatgaaaa gccggttccg gcgctctcac ctttcctttt tctcccaatt tttcagttga
     5881 aaaaggtata tgcgtcaggc gacctctgaa attaacaaaa aatttccagt catcgaattt
     5941 gattctgtgc gatagcgccc ctgtgtgttc tcgttatgtt gaggaaaaaa ataatggttg
     6001 ctaagagatt cgaactcttg catcttacga tacctgagta ttcccacagt tgggggatct
     6061 cgactctagc tagaggatca attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
     6121 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6181 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6241 ggaaacctgt cgtgccagct gataacttcg tataatgtat gctatacgaa gttattaggt
     6301 ctgaagagga gtttacgtcc agccaagcta gcttggctgc aggtcgagcg gccgcgatcc
     6361 ggaaccctta atataacttc gtataatgta tgctatacga agttatcagc tgcattaatg
     6421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6481 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6541 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6601 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6661 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6721 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6781 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6841 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6901 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6961 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7021 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7081 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7141 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7201 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7261 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7321 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7381 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7441 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7501 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7561 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7621 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7681 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7741 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7801 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7861 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7921 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7981 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8041 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8101 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8161 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8221 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8281 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8341 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8401 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     8461 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     8521 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     8581 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     8641 agtgcaccat aacgcattta agcataaaca cgcactatgc cgttcttctc atgtatatat
     8701 atatacaggc aacacgcaga tataggtgcg acgtgaacag tgagctgtat gtgcgcagct
     8761 cgcgttgcat tttcggaagc gctcgttttc ggaaacgctt tgaagttcct attccgaagt
     8821 tcctattctc tagctagaaa gtataggaac ttcagagcgc ttttgaaaac caaaagcgct
     8881 ctgaagacgc actttcaaaa aaccaaaaac gcaccggact gtaacgagct actaaaatat
     8941 tgcgaatacc gcttccacaa acattgctca aaagtatctc tttgctatat atctctgtgc
     9001 tatatcccta tataacctac ccatccacct ttcgctcctt gaacttgcat ctaaactcga
     9061 cctctacatt ttttatgttt atctctagta ttactcttta gacaaaaaaa ttgtagtaag
     9121 aactattcat agagtgaatc gaaaacaata cgaaaatgta aacatttcct atacgtagta
     9181 tatagagaca aaatagaaga aaccgttcat aattttctga ccaatgaaga atcatcaacg
     9241 ctatcacttt ctgttcacaa agtatgcgca atccacatcg gtatagaata taatcgggga
     9301 tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt
     9361 ggagtcaggc tttttttatg gaagagaaaa tagacaccaa agtagccttc ttctaacctt
     9421 aacggaccta cagtgcaaaa agttatcaag agactgcatt atagagcgca caaaggagaa
     9481 aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc tcgggatgca tttttgtaga
     9541 acaaaaaaga agtatagatt ctttgttggt aaaatagcgc tctcgcgttg catttctgtt
     9601 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     9661 gttttacaaa aatgaagcac agattcttcg ttggtaaaat agcgctttcg cgttgcattt
     9721 ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca
     9781 tttttgttct acaaaatgaa gcacagatgc ttcgttgctt gcatgcctgc aggtcgagat
     9841 ccgggatcga agaaatgatg gtaaatgaaa taggaaatca aggagcatga aggcaaaaga
     9901 caaatataag ggtcgaacga aaaataaagt gaaaagtgtt gatatgatgt atttggcttt
     9961 gcggcgccga aaaaacgagt ttacgcaatt gcacaatcat gctgactctg tggcggaccc
    10021 gcgctcttgc cggcccggcg ataacgctgg gcgtgaggct gtgcccggcg gagttttttg
    10081 cgcctgcatt ttccaaggtt taccctgcgc taaggggcga gattggagaa gcaataagaa
    10141 tgccggttgg ggttgcgatg atgacgacca cgacaactgg tgtcattatt taagttgccg
    10201 aaagaacctg agtgcatttg caacatgagt atactagaag aatgagccaa gacttgcgag
    10261 acgcgagttt gccggtggtg cgaacaatag agcgaccatg accttgaagg tgagacgcgc
    10321 ataaccgcta gagtactttg aagaggaaac agcaataggg ttgctaccag tataaataga
    10381 caggtacata caacactgga aatggttgtc tgtttgagta cgctttcaat tcatttgggt
    10441 gtgcacttta ttatgttaca atatggaagg gaactttaca cttctcctat gcacatatat
    10501 taattaaagt ccaatgctag tagagaaggg gggtaacacc cctccgcgct cttttccgat
    10561 ttttttctaa accgtggaat atttcggat
//
