LOCUS       sequence-181682-       13580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in eukaryotic cells and generating stable cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13580)
  AUTHORS   Gao W, Yang J, Liu W, Wang Y, Shao F
  TITLE     Site-specific phosphorylation and microtubule dynamics control Pyrin
            inflammasome activation.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):E4857-66. doi: 
            10.1073/pnas.1601700113. Epub 2016 Aug 1.
  PUBMED    27482109
REFERENCE   2  (bases 1 to 13580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1601700113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-08-16- 16"; volume: "113"; issue: "33"; pages: 
            "E4857-66"
COMMENT     Sequence Label: pWPI-IRES-eGFP-Mefv iso1
FEATURES             Location/Qualifiers
     source          1..13580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1209..1232
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3704..3821
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3870..4437
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3888..4439
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4032..4049)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4259..4278
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4442..4444,4445..4447,4448..5158)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4442..4444,4445..4447,4448..5158)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4487..4508)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4748..4767)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5095..5116
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5237..5253)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5238..5254)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    5267..5855
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5320..5340)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5738..5749)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5999..6032)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6052..6232
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6257..6275)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6258..6275)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6565..6584)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6798..6817)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6917..6939
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6977..6995)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7063..7167
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7168..7236,7237..8028)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7386..7405)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8199..8787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8688..8707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8941..8958
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9028..9048)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9033..9362
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9213..9348
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10496..10561
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10691..10711
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11136..11270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11173..11192)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11227..11246
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11438..12071
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12118..12243
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12736..12969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13154..13198
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaatttcgac atttaaattt
     1201 aatcgatgga ctacaaggac gacgatgaca agttaattaa gatggccaag acccttggtg
     1261 atcatctgct aaacaccctg gaggagctgc tgccctatga ctttgagaag ttcaagttta
     1321 agctacagaa caccagcctg gagaagggcc actctaagat cccccggggc catatgcaga
     1381 tggccaggcc agtaaagctg gccagccttc tgataaccta ctacggggag gagtatgctg
     1441 taaggctgac cctgcagatc ctgcgggcca ccaaccagcg ccaactagca gaggagcttc
     1501 gcaaagccac aggcacagaa catttaattg aagaaaatag agttggtggt tctgtccagt
     1561 cttctgtaga gaataaagcc aagagtgtga aggtaccaga tgtccctgaa ggtgatggga
     1621 cacagcaaaa caatgatgaa tcagacaccc ttccatccag ccaggctgaa gtggggaagg
     1681 gaccccagaa gaagtcactg accaaaagga aggatcagag gggccccgag agcctggact
     1741 cacagaccaa gccatggaca aggagcacag cgcctctcta taggagaacc cagggcaccc
     1801 agtccccagg ggataaggag agcacagcaa gtgcccagct ccgcaggaat gtcagctctg
     1861 cagggaggct tcaaggactt tacaacaatg ccccagggag gagagaaagc aagaaagccg
     1921 aagtgtatgt atatttgcct tcaggaaaga agcgacccag gagtcttgaa attaccactt
     1981 attcaagaga aggagaaccc ccaaattcag aagttcttcc aactcaagag gaaacaagaa
     2041 atgggagtct cattcgcatg agaacagcta ctctgaatgg aaggactacg ggggctttag
     2101 aaaagggcac agggattcca gagcattcca tggtgctgga tgaaaaaaca ttcagaaaca
     2161 tgtcttccaa aacatcgttg attggggagg agagatgccc tacatcatgg acggaaaatg
     2221 gaaatgggag tccagaaacc acagagtcct cgggagagac ggctggcagc atactctctg
     2281 atccagaagt gcctttgtca ctatgtgaaa aaccagctaa aactccagaa gacccagcat
     2341 ccttaggaca ggccgcttgt gaaggtaccc tccttcaggt ctcaagaaaa tgtgatgcag
     2401 gcccagaaaa atgcacattt acacatcttg cactacaggt tgcatcaaat ttcggaaggt
     2461 cacaggacaa ggctgtgtgc cctctttgcc acacccagga aggagacctg cgtggtgata
     2521 cctgtgtgca aagttcctgt agctgctcca ttgctcctgg ggatcccaag gcctccggca
     2581 gatgctccat atgctttcag tgccaaggct tacttgccag aaagagctgc gaagcccaga
     2641 gcccccagtc cctaccacag tgccctcgtc acatgaagca ggtgctgctg cttttctgtg
     2701 aggaccacag ggagcccatc tgcctcatct gcaggctgag cctggagcat caaggacatc
     2761 gagtgcgccc catagaggag gctgcactag agtacaagga gcagatccgg gagcagctgg
     2821 agcgcctgcg ggagatgagg ggatatgtgg aggagcacag gctacaggga gacaagaaaa
     2881 cagacgattt cctgaaacaa acagaaatcc agaagcagaa gatttcatgt ccacttgaga
     2941 agctatacca actcctggag aagcaagaac aactctttgt gacctggcta caggagctaa
     3001 gccagaccat cagcaaggtc agagagacat actacacccg ggtttcccta ctggatgaga
     3061 tgattgaaga actagaggcc aagcaagacc agccagagtg ggacctcatg caggacattg
     3121 gaatcacctt gcacagggct aagatgatgt ctgcctctga gctgttggac actcctccag
     3181 gtgttaaaga aaagcttcat ctgctctacc agaagtcaaa gtctgtggag aagaatatgc
     3241 agtgtttctc agaaatgctg agctctgaaa tggcattcag cgcttcagat gtagcaaaat
     3301 gggaagggcg ccagcctagc gccacacagg tccagggctt ggttcccacc gttcacctaa
     3361 agtgtgatgg tgcacacaca caggactgtg atgtagtatt ttacccagaa cgggaagctg
     3421 gaggatcaga acctaaagat taccttcatc ctcagccagc tcaagacaca cctgagctac
     3481 atgagatcca ttctcggaac aacaaaagaa aatttaaatc tttcctgaaa tggaaacctt
     3541 cattcattgg gagcaccttg ggagaagccc acagatccgc actcccagct aggcattctt
     3601 cttcctgcag ttaccgtgat ttggaccaag cagctgctca ccccaacctc atcttctcga
     3661 tgatatgagg cgcgcccgat taatctcgac ggtatcggtt aacttttaaa agaaaagggg
     3721 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     3781 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc
     3841 tcgaggttta aactacgggc tgcaggaatt ccgccccccc ccncctaacg ttactggccg
     3901 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     3961 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     4021 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     4081 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     4141 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     4201 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     4261 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     4321 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     4381 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatac
     4441 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     4501 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     4561 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     4621 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     4681 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     4741 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     4801 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     4861 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     4921 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     4981 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     5041 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     5101 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
     5161 cggactcaga tctcgactag ctagtagcta gctagctagt cgagctcaag cttcgaattc
     5221 gatatcaagc ttatcgcgat accgtcgacc tcgagggaat tccgataatc aacctctgga
     5281 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5341 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5401 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5461 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5521 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5581 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5641 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     5701 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5761 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     5821 gacgagtcgg atctcccttt gggccgcctc cccgcatcgg gaattcgagc tcggtacctt
     5881 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5941 gactggaagg gctaattcac tcccaacgaa gacaagatgg gatcaattca ccatgggaat
     6001 aacttcgtat agcatacatt atacgaagtt atgctgcttt ttgcttgtac tgggtctctc
     6061 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6121 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6181 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     6241 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     6301 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     6361 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     6421 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
     6481 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     6541 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     6601 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     6661 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     6721 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     6781 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     6841 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     6901 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6961 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     7021 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7081 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7141 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7201 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7261 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7321 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7381 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7441 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7501 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7561 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7621 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7681 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7741 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7801 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7861 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7921 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7981 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8041 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8101 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8161 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8221 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8281 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8341 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8401 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8461 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8521 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8581 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8641 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8701 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8761 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8821 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8881 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8941 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     9001 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     9061 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     9121 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     9181 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     9241 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     9301 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     9361 aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg
     9421 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg
     9481 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat
     9541 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag
     9601 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt
     9661 gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc
     9721 atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc
     9781 tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg
     9841 gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg
     9901 caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct
     9961 ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat
    10021 cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag
    10081 tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg
    10141 ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc
    10201 gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg
    10261 attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag
    10321 gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt
    10381 ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta
    10441 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
    10501 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
    10561 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
    10621 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
    10681 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
    10741 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
    10801 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
    10861 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
    10921 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
    10981 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
    11041 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    11101 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    11161 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    11221 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    11281 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    11341 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    11401 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
    11461 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
    11521 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
    11581 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
    11641 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
    11701 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
    11761 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
    11821 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
    11881 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    11941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    12001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    12061 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
    12121 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
    12181 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
    12241 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
    12301 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
    12361 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
    12421 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
    12481 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
    12541 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
    12601 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
    12661 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
    12721 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
    12781 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
    12841 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
    12901 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
    12961 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
    13021 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
    13081 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
    13141 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
    13201 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
    13261 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
    13321 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
    13381 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
    13441 cggatctcga cggtatcgat cacgagacta gcctcgacca tcgatgtcga cgataagctt
    13501 tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg
    13561 tcttgaaagg agtgggaatt
//
