LOCUS       sequence-181684-        7345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic 
            interneurons under the control of the hDlx enhancer element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu 
            Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith 
            GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, 
            Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, 
            Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G,
            Fishell G
  TITLE     A viral strategy for targeting and manipulating interneurons across 
            vertebrate species.
  JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.
  PUBMED    27798629
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430."
COMMENT     Sequence Label: pAAV-hDlx-GqDREADD-dTomato-Fishell-4
FEATURES             Location/Qualifiers
     source          1..7345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     71..89
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(220..240)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(253..309)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2098..2124)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2174..2193)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          2176..2308
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     repeat_region   3027..3156
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(3218..3806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3298..3317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3977..4768,4769..4837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4600..4619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4838..4942)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5066..5088)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5188..5207
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5224..5679)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5361..5382)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5573..5592
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   5754..5894
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5765..5894
                     /label=AAV2 ITR
     primer_bind     complement(5944..5963)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    5973..6054
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5998..6017
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    6223..6811
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6329..6340
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6738..6758
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(6866..7345,1..219))
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(6949..6972)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(7147..7166)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cacgtacgcc ttggagccgt acatgaactg gggggacagg atgtcccagg cgaagggcag
       61 ggggccgccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc
      121 gccctcgccc tcgatctcga actcgtggcc gttcatggag ccctccatgc gcaccttgaa
      181 gcgcatgaac tctttgatga cctcctcgcc cttgctcacc accttcctct tcttcttggg
      241 ggccattcta gatggcccgg gattctcttc gacatcccct gcttgtttca acagggagaa
      301 gttagtggct ccgcttccgt tatctccgga caaggcctgc tcgggtgcgc gcttgtgaaa
      361 aatgaccgac tgtctctgct ggtactgctg cttgcgcctc ttttttttgt cacactggca
      421 cagcagcagc atcttgaaag tggttctgaa tgttttgttg cacagagcat agcacacggg
      481 gttcacggtg ctgttgatgt agcacagcca gtagcccaga ttccaaaagg ttttgggtat
      541 gcagctgtca caaaaggtgt tcaccagaac catgatgttg tatggggtcc aagtgatgat
      601 gaaggcaagc aagatcgcac tgagggtctg ggccgctttc ttctccttga ccagggacat
      661 ccttttccgc ttagtgatct gacttctggt cttcagagca aacctcttgg ccagagtggc
      721 ttccttgaag gacagaggta gagtggccgt gctcttaccc actgaggagt tgacgtcaga
      781 agtcttagct gtgtccacgg ctgactctag ctggatggga agcttggaga agctttttgg
      841 aaaactgcct ccatcgtcca cgctcttctg ggcctgcagc ttgtcggctt tcctctccaa
      901 gtccaccatc cccagctcct cctcaggcac ctgcaggttg tccgatgagg gtaacttggt
      961 ggagttgagg atggtgctgt gacccggaag cttgagcacg atggagtaga tggctctcgt
     1021 ctcggagcca atgtcctcct cgtcggagga ggcggagttc tccagggagg cagcagcatc
     1081 attgttgttc caactgtcac tgctgctgtg gtcttggtcc atctgctcgg agctgggttt
     1141 ccagctcttg gttgtgaacc agaagtggca gcggccatac ttcctcctgt tggagcgttt
     1201 catgctttgc tgttgaagtt cgtaactgct gcagcttcga gaactgcccg tggggtggac
     1261 aaagttttct gtctctgcct ctgtcccaga ggcttgcagg ccagcaagct ctttggtacg
     1321 cttttcagtt tccttataga tcctccagta taaaatagtc ataatggtga caggcatata
     1381 aaaaccagcg atggctgtgc caaaagtaat ggtgggctca ctgaggaact gaatgaagca
     1441 ctctcccgga ggcacagttc tctttccaac aaagtattgc cagaacaaga tggcaggagc
     1501 ccaaaggaca aaggagatga cccaagccag accgatcatc acaccggctc tctttgttgt
     1561 tcgtttggct cggtacgtga gcggcctcgt gatggaaaag tatctgtcaa agctgatgac
     1621 cagaagattc ataacagagg cattgctggc tacgcagtca atggcaagcc agaggtcaca
     1681 ggccaagttc cctaaggccc atcgattcat gatgatgtag gtcgtaaaca gattcattga
     1741 aatgaccccg ataatcagat cggcacaggc caggcttaag aggaagtagt tgttgaccgt
     1801 cttcagctgc ttgttgacct taaatgacac aattaccagg atgttgccga tgatggtcac
     1861 caaggccagg atgcccgtta agaaagcgat gaagaccact tgccagacgg tatgacctcc
     1921 cagagggtca tcggtggtac cgtctggaga ggagaaattg ccagctgctc gagaaacatt
     1981 gtagctgccg aaatgagtga cggttcccgg gggcagccct gcatcggagg ggctgtgtat
     2041 ccaggaggag ctgatgtttg gaaacaaagg cgaggttgta ctgttattgt gcaaggtagc
     2101 gtaatctgga acatcgtatg ggtacatggt ggcgctagct ttcttaagag ctgtaattga
     2161 actgggagtg gacacctgtg gagagaaagg caaagtggat gtcagtaaga ccaataggtg
     2221 cctatcagaa acgcaagagt cttctctgtc tcgacaagcc cagtttctat tggtctcctt
     2281 aaacctgtct tgtaaccttg atacttacct gcccagtgcc tcacgaccaa cttctgcagc
     2341 ttaaggcgcc gcgctctgct tctggaaggc tgcgctcctc gacctgcagg ctagaagcaa
     2401 atgtaagcaa tagatggctc tgccctgact tttatgccca gcccgggctg caggaattcg
     2461 atatcaagct tcctactgtg aaactttggg ttcgtagctc cccaggatca attctgaaca
     2521 aagcctccag ctgcagtgcc atccaatttg aagcagacat tggggacaat ttaaggtttt
     2581 tatccacaag aaggtttttt tccattctct taaatgcagc cataattaga gtaatttttc
     2641 atgtagcccg ctgattacag cgtttttacc gtcaaagata attacctgta attttcttcc
     2701 acttttaata ctaaaaagcc atctttattt agattcagga acaggaaagg cgaaacaaaa
     2761 gagggaaatt attctgttat tcatacacaa attgcagaga cgtaggacct aaaattgaaa
     2821 attaaccaaa attataatgc tgaaaagaat ggaagaggct gcagaagtac agctggtagt
     2881 ggggctttgc tgaattattc aaattacgtt agggagaatg ctaccataca tcgaaaccaa
     2941 cactaatttt tcgttaaaaa aaattcacca gatgcatgcc aaaccactgt gagtgcataa
     3001 cattctgaat aaacgcgtgc ggccgcagga acccctagtg atggagttgg ccactccctc
     3061 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggcgg
     3121 cctcagtgag cgagcgagcg cgcagctgcc tgcaggacat gtgagcaaaa ggccagcaaa
     3181 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3241 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3301 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3361 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3421 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3481 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3541 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3601 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3661 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3721 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3781 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3841 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3901 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3961 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4021 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4081 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4141 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4201 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4261 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4321 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4381 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4441 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4501 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4561 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4621 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4681 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4741 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4801 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4861 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4921 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     4981 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     5041 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     5101 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     5161 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     5221 ataaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     5281 cattttttaa ccaatagacc gaaatcggca aaatccctta taaatcaaaa gaatagcccg
     5341 agatagagtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     5401 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     5461 ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     5521 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     5581 aagcgaaagg agcgggcgct aaggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     5641 ccacacccgc cgcgcttaat gcgccgctac agggcgcgta ctatggttgc tttgacgtat
     5701 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gcccctgcag
     5761 gcagctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac
     5821 ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat
     5881 cactaggggt tcctgcggcc gctcggtccg cacgtgcatg cggtatcgat aagctgatac
     5941 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     6001 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     6061 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     6121 aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg gccagcttga
     6181 tggccgctat cacagatctc tcgagcagcg ctcggtatcg atgcggggag gcggcccaaa
     6241 gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc agagccggca
     6301 gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg
     6361 tcccgcgcag aatccaggtg gcaacatagg cgagcagcca aggaaaggac gatgatttcc
     6421 ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa
     6481 ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa
     6541 ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc
     6601 acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg
     6661 atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca
     6721 ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc
     6781 tttcacaaat tttgtaatcc agaggttgat tatcgataag cttgatatcg aattccctgc
     6841 aggggtgggg cgcgccgtcg acttacttgt acagctcgtc catgccgtac aggaacaggt
     6901 ggtggcggcc ctcggagcgc tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg
     6961 tgatgtccag cttggtgtcc acgtagtagt agccgggcag ttgcacgggc ttcttggcca
     7021 tgtagatggt cttgaactcc accaggtagt ggccgccgtc cttcagcttc agggcctggt
     7081 ggatctcgcc cttcagcacg ccgtcgcggg ggtacaggcg ctcggtggag gcctcccagc
     7141 ccatggtctt cttctgcatt acggggccgt cgggggggaa gttggtgccg cgcatcttca
     7201 ccttgtagat cagcgtgccg tcctgcaggg aggagtcctg ggtcacggtc accagaccgc
     7261 cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcttgt
     7321 aatcggggat gtcggcgggg tgctt
//
