LOCUS       sequence-181685-        3134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA 
            and FSH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB2_L_12_syn_A_H
FEATURES             Location/Qualifiers
     source          1..3134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     47..64
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    181..202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(217..222,223..240,241..247)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    255..271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     260..282
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     279..295
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     279..295
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     314..333
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        314..332
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(577..595)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(578..595)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(609..626)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(609..625)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(618..640)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(766..1221)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(903..924)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1115..1134
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1248..1352
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1353..1421,1422..2213)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1571..1590)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2439..3027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2928..2947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
       61 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      121 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      181 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      241 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      301 ttacgccaag ctctaatacg actcactata gggaaagctc ggtaccacgc atgctgcaga
      361 cgcgttacgt atcggatcca gaattcgtga tggaagacgc catggttggg agaccctcga
      421 gggatccggt ctccgatcgc gaaaaaaccc cgccctgtca ggggcggggt tttttgcgct
      481 ccgcgtcttc gatcatctga attcgtcgac aagcttctcg agcctaggct agctctagac
      541 cacacgtgtg ggggcccgag ctcgcggccg ctgtattcta tagtgtcacc taaatggccg
      601 cacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      661 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      721 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt gtaagcgtta
      781 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
      841 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
      901 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
      961 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     1021 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     1081 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     1141 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     1201 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1261 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1321 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1381 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1441 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1501 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1561 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1621 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1681 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1741 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1801 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1861 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     1921 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1981 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2041 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2101 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2161 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2221 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2281 tctaggtgaa gatccttttt gataatctca tgaacaataa aactgtctgc ttacataaac
     2341 agtaatacaa ggggtgtaga tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2401 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2461 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2521 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2581 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2641 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2701 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2761 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2821 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2881 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2941 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3001 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3061 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3121 aaccgtatta ccgc
//
