LOCUS       sequence-181686-       10641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression in eukaryotic cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10641)
  AUTHORS   Gao W, Yang J, Liu W, Wang Y, Shao F
  TITLE     Site-specific phosphorylation and microtubule dynamics control Pyrin
            inflammasome activation.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):E4857-66. doi: 
            10.1073/pnas.1601700113. Epub 2016 Aug 1.
  PUBMED    27482109
REFERENCE   2  (bases 1 to 10641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1601700113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-08-16- 16"; volume: "113"; issue: "33"; pages: 
            "E4857-66"
COMMENT     Sequence Label: pLenti-NIrD-Mefv iso1
FEATURES             Location/Qualifiers
     source          1..10641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        63..110
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             129..527
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    570..1158
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(623..643)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1041..1052)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1244..1477
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1549..1670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1586..1605)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1640..1659
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1710..1845
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1865..1884)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1866..1884)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1894..1911)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1894..1910)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1903..1925)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2052..2507
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2139..2158)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2349..2370
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2533..2637
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2638..2706,2707..3498)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2856..2875)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3669..4257
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4158..4177
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4411..4428
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4545..4566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4581..4586,4587..4604,4605..4611)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4619..4635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4624..4646
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4643..4659
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4643..4659
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4678..4698
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4680..4698
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4726..4952
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4953..5133
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5180..5305
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5798..6031
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6216..6260
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6527..6643
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6687..6705
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6723..6741
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6759..6777
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6795..6813
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6825..6863
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6860..6884
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             9345..9410
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(9440..9460)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9445..9774
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9625..9760
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(9794..10411,10412..10417,10418..10453,10454..10456,
                     10457..10492,10493..10495,10496..10531,10532..10540)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 cggcgggttt ctgacatccg gcgggtgact cacaacccca gaaacagaca tctcgagcac
       61 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
      121 actaaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
      181 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
      241 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
      301 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
      361 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
      421 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
      481 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca cttcgtggcc
      541 gaggagcagg actgagaatt ccagtcgaca atcaacctct ggattacaaa atttgtgaaa
      601 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
      661 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
      721 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
      781 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
      841 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
      901 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
      961 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     1021 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     1081 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     1141 tttgggccgc ctccccgcct ggaattcgag ctcggtactt taagaccaat gacttacaag
     1201 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     1261 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     1321 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     1381 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     1441 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     1501 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     1561 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1621 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     1681 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     1741 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     1801 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     1861 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     1921 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     1981 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     2041 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2101 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2161 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2221 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2281 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2341 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2401 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2461 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     2521 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2581 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2641 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2701 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2761 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2821 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2881 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2941 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3001 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3061 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3121 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3181 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3241 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3301 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3361 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3421 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3481 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3541 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3601 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3661 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3721 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3781 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3841 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3901 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3961 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4021 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4081 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4141 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4201 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4261 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4321 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4381 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4441 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4501 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     4561 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     4621 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     4681 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     4741 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     4801 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     4861 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     4921 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     4981 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5041 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5101 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     5161 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     5221 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     5281 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     5341 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     5401 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     5461 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     5521 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     5581 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     5641 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     5701 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     5761 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     5821 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     5881 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     5941 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     6001 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     6061 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     6121 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     6181 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     6241 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     6301 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     6361 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     6421 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     6481 acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag
     6541 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     6601 aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgata aaggaaccaa
     6661 ttcagtcgac tggatcccga gtttactccc tatcagtgat agagaacgta tgtcgagttt
     6721 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     6781 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt
     6841 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgccggc cggccaatgg
     6901 ccaagaccct tggtgatcat ctgctaaaca ccctggagga gctgctgccc tatgactttg
     6961 agaagttcaa gtttaagcta cagaacacca gcctggagaa gggccactct aagatccccc
     7021 ggggccatat gcagatggcc aggccagtaa agctggccag ccttctgata acctactacg
     7081 gggaggagta tgctgtaagg ctgaccctgc agatcctgcg ggccaccaac cagcgccaac
     7141 tagcagagga gcttcgcaaa gccacaggca cagaacattt aattgaagaa aatagagttg
     7201 gtggttctgt ccagtcttct gtagagaata aagccaagag tgtgaaggta ccagatgtcc
     7261 ctgaaggtga tgggacacag caaaacaatg atgaatcaga cacccttcca tccagccagg
     7321 ctgaagtggg gaagggaccc cagaagaagt cactgaccaa aaggaaggat cagaggggcc
     7381 ccgagagcct ggactcacag accaagccat ggacaaggag cacagcgcct ctctatagga
     7441 gaacccaggg cacccagtcc ccaggggata aggagagcac agcaagtgcc cagctccgca
     7501 ggaatgtcag ctctgcaggg aggcttcaag gactttacaa caatgcccca gggaggagag
     7561 aaagcaagaa agccgaagtg tatgtatatt tgccttcagg aaagaagcga cccaggagtc
     7621 ttgaaattac cacttattca agagaaggag aacccccaaa ttcagaagtt cttccaactc
     7681 aagaggaaac aagaaatggg agtctcattc gcatgagaac agctactctg aatggaagga
     7741 ctacgggggc tttagaaaag ggcacaggga ttccagagca ttccatggtg ctggatgaaa
     7801 aaacattcag aaacatgtct tccaaaacat cgttgattgg ggaggagaga tgccctacat
     7861 catggacgga aaatggaaat gggagtccag aaaccacaga gtcctcggga gagacggctg
     7921 gcagcatact ctctgatcca gaagtgcctt tgtcactatg tgaaaaacca gctaaaactc
     7981 cagaagaccc agcatcctta ggacaggccg cttgtgaagg taccctcctt caggtctcaa
     8041 gaaaatgtga tgcaggccca gaaaaatgca catttacaca tcttgcacta caggttgcat
     8101 caaatttcgg aaggtcacag gacaaggctg tgtgccctct ttgccacacc caggaaggag
     8161 acctgcgtgg tgatacctgt gtgcaaagtt cctgtagctg ctccattgct cctggggatc
     8221 ccaaggcctc cggcagatgc tccatatgct ttcagtgcca aggcttactt gccagaaaga
     8281 gctgcgaagc ccagagcccc cagtccctac cacagtgccc tcgtcacatg aagcaggtgc
     8341 tgctgctttt ctgtgaggac cacagggagc ccatctgcct catctgcagg ctgagcctgg
     8401 agcatcaagg acatcgagtg cgccccatag aggaggctgc actagagtac aaggagcaga
     8461 tccgggagca gctggagcgc ctgcgggaga tgaggggata tgtggaggag cacaggctac
     8521 agggagacaa gaaaacagac gatttcctga aacaaacaga aatccagaag cagaagattt
     8581 catgtccact tgagaagcta taccaactcc tggagaagca agaacaactc tttgtgacct
     8641 ggctacagga gctaagccag accatcagca aggtcagaga gacatactac acccgggttt
     8701 ccctactgga tgagatgatt gaagaactag aggccaagca agaccagcca gagtgggacc
     8761 tcatgcagga cattggaatc accttgcaca gggctaagat gatgtctgcc tctgagctgt
     8821 tggacactcc tccaggtgtt aaagaaaagc ttcatctgct ctaccagaag tcaaagtctg
     8881 tggagaagaa tatgcagtgt ttctcagaaa tgctgagctc tgaaatggca ttcagcgctt
     8941 cagatgtagc aaaatgggaa gggcgccagc ctagcgccac acaggtccag ggcttggttc
     9001 ccaccgttca cctaaagtgt gatggtgcac acacacagga ctgtgatgta gtattttacc
     9061 cagaacggga agctggagga tcagaaccta aagattacct tcatcctcag ccagctcaag
     9121 acacacctga gctacatgag atccattctc ggaacaacaa aagaaaattt aaatctttcc
     9181 tgaaatggaa accttcattc attgggagca ccttgggaga agcccacaga tccgcactcc
     9241 cagctaggca ttcttcttcc tgcagttacc gtgatttgga ccaagcagct gctcacccca
     9301 acctcatctt ctcgatgata tgaggcgcgc caggatcccg ggctgactac aaagaccatg
     9361 acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag tagacgcgtc
     9421 gaccagtggt cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     9481 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     9541 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9601 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     9661 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     9721 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta
     9781 tcgctagctc gagatgtcta gactggacaa gagcaaagtc ataaacggag ctctggaatt
     9841 actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     9901 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc
     9961 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
    10021 agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg
    10081 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
    10141 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
    10201 cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga
    10261 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
    10321 gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
    10381 cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga
    10441 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
    10501 cgctcttgac gattttgacc ttgacatgct ccccgggtag atcgataccg cgacctcgaa
    10561 tcactagtcc ggcgggyttc tgacatccgg cgggtttctg acatccggcg ggtttctgac
    10621 atccggcggg tttctgacat c
//
