LOCUS       sequence-181689-       12947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 12947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP12-FilaminA-C-14
FEATURES             Location/Qualifiers
     source          1..12947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1512
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     polyA_signal    9675..9796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9712..9731)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9766..9785
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9803..10258)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9940..9961)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10152..10171
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10285..10389
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10391..10748
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10391..10582
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10414..10434)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10599..10734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10783..11577
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10783..11577
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10837..10856)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11447..11466
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11765..11784)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11809..11856
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12185..12773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12674..12693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgtcggt accgctgact acctcagcat tcggccacgc gtttctggct
      601 aactgtgaac gcgagcagat ccacctggcg ggctccattc agccgcacgg tatcctgctg
      661 gctgtgaaag agccggacaa cgtggtgatc caggcttcta ttaacgctgc ggagttcctg
      721 aacaccaact ctgttgttgg ccgtccgctg cgtgacctgg gcggcgatct gcctttgcag
      781 atcctgccgc acctgaacgg cccgctgcac ctggctccga tgaccctgcg ttgtaccgtg
      841 ggttctccgc cgcgtcgtgt ggactgtacc attcatcgtc cgtctaacgg cggcctgatc
      901 gtagaactgg aaccagcaac caagaccact aacattgcgc cggctctgga cggtgcgttt
      961 catcgtatca cttcttcatc ctccctgatg ggcctgtgtg acgaaaccgc gactattatc
     1021 cgtgagatta ctggctacga ccgtgtgatg gtagtacgtt tcgatgaaga gggtaatggc
     1081 gaaattctgt ccgaacgtcg tcgtgcggac ctggaagcgt tcctgggtaa ccgctacccg
     1141 gcgtctacta ttccgcagat cgctcgtcgc ctgtacgaac ataaccgtgt tcgcctgctg
     1201 gtagatgtga actatactcc ggttccgcta cagccgcgca tcagcccgct gaacggtcgt
     1261 gatctggata tgtccctgtc ttgcctgcgc tctatgtccc cgatccacca gaaatacatg
     1321 caggacatgg gcgttggcgc gaccctggtt tgctctctga tggtgtctgg tcgtctgtgg
     1381 ggtctgatcg cttgccacca ctacgaaccg cgcttcgttc cgttccacat tcgcgctgct
     1441 ggcgaagcgc tggcggaaac ttgtgcgatc cgcatcgcga cgctggagag ctttgcacag
     1501 tctcagtcca aaggtggcgg aagcggcggc ggaagcggtg gcggaagttc cggagggagc
     1561 ggggctggag ggagcggggg tggcagcggt agtagctccc actctcgggc gggccagagc
     1621 gcagcaggcg cggctccggg cggcggcgtc gacacgcggg acgccgagat gccggccacc
     1681 gagaaggacc tggcggagga cgcgccatgg aagaagatcc agcagaacac tttcacgcgc
     1741 tggtgcaacg agcacctgaa gtgcgtgagc aagcgcatcg ccaacctgca gacggacctg
     1801 agcgacgggc tgcggcttat cgcgctgttg gaggtgctca gccagaagaa gatgcaccgc
     1861 aagcacaacc agcggcccac tttccgccaa atgcagcttg agaacgtgtc ggtggcactc
     1921 gagttcctgg accgcgagag catcaaactg gtgtccatcg acagcaaggc catcgtggac
     1981 gggaacctga agctgatcct gggcctcatc tggaccctga tcctgcacta ctccatctcc
     2041 atgcccatgt gggacgagga ggaggatgag gaggccaaga agcagacccc caagcagagg
     2101 ctcctgggct ggatccagaa caagctgccg cagctgccca tcaccaactt cagccgggac
     2161 tggcagagcg gccgggccct gggcgccctg gtggacagct gtgccccggg cctgtgtcct
     2221 gactgggact cttgggacgc cagcaagccc gttaccaatg cgcgagaggc catgcagcag
     2281 gcggatgact ggctgggcat cccccaggtg atcacccccg aggagattgt ggaccccaac
     2341 gtggacgagc actctgtcat gacctacctg tcccagttcc ccaaggccaa gctgaagcca
     2401 ggggctccct tgcggcccaa actgaacccg aagaaagccc gtgcctacgg gccaggcatc
     2461 gagcccacag gcaacatggt gaagaagcgg gcagagttca ctgtggagac cagaagtgct
     2521 ggccagggag aggtgctggt gtacgtggag gacccggccg gacaccagga ggaggcaaaa
     2581 gtgaccgcca ataacgacaa gaaccgcacc ttctccgtct ggtacgtccc cgaggtgacg
     2641 gggactcata aggttactgt gctctttgct ggccagcaca tcgccaagag ccccttcgag
     2701 gtgtacgtgg ataagtcaca gggtgacgcc agcaaagtga cagcccaagg tcccggcctg
     2761 gagcccagtg gcaacatcgc caacaagacc acctactttg agatctttac ggcaggagct
     2821 ggcacgggcg aggtcgaggt tgtgatccag gaccccatgg gacagaaggg cacggtagag
     2881 cctcagctgg aggcccgggg cgacagcaca taccgctgca gctaccagcc caccatggag
     2941 ggcgtccaca ccgtgcacgt cacgtttgcc ggcgtgccca tccctcgcag cccctacact
     3001 gtcactgttg gccaagcctg taacccgagt gcctgccggg cggttggccg gggcctccag
     3061 cccaagggtg tgcgggtgaa ggagacagct gacttcaagg tgtacacaaa gggcgctggc
     3121 agtggggagc tgaaggtcac cgtgaagggc cccaagggag aggagcgcgt gaagcagaag
     3181 gacctggggg atggcgtgta tggcttcgag tattacccca tggtccctgg aacctatatc
     3241 gtcaccatca cgtggggtgg tcagaacatc gggcgcagtc ccttcgaagt gaaggtgggc
     3301 accgagtgtg gcaatcagaa ggtacgggcc tggggccctg ggctggaggg cggcgtcgtt
     3361 ggcaagtcag cagactttgt ggtggaggct atcggggacg acgtgggcac gctgggcttc
     3421 tcggtggaag ggccatcgca ggctaagatc gaatgtgacg acaagggcga cggctcctgt
     3481 gatgtgcgct actggccgca ggaggctggc gagtatgccg ttcacgtgct gtgcaacagc
     3541 gaagacatcc gcctcagccc cttcatggct gacatccgtg acgcgcccca ggacttccac
     3601 ccagacaggg tgaaggcacg tgggcctgga ttggagaaga caggtgtggc cgtcaacaag
     3661 ccagcagagt tcacagtgga tgccaagcac ggtggcaagg ccccacttcg ggtccaagtc
     3721 caggacaatg aaggctgccc tgtggaggcg ttggtcaagg acaacggcaa tggcacttac
     3781 agctgctcct acgtgcccag gaagccggtg aagcacacag ccatggtgtc ctggggaggc
     3841 gtcagcatcc ccaacagccc cttcagggtg aatgtgggag ctggcagcca ccccaacaag
     3901 gtcaaagtat acggccccgg agtagccaag acagggctca aggcccacga gcccacctac
     3961 ttcactgtgg actgcgccga ggctggccag ggggacgtca gcatcggcat caagtgtgcc
     4021 cctggagtgg taggccccgc cgaagctgac atcgacttcg acatcatccg caatgacaat
     4081 gacaccttca cggtcaagta cacgccccgg ggggctggca gctacaccat tatggtcctc
     4141 tttgctgacc aggccacgcc caccagcccc atccgagtca aggtggagcc ctctcatgac
     4201 gccagtaagg tgaaggccga gggccctggc ctcagtcgca ctggtgtcga gcttggcaag
     4261 cccacccact tcacagtaaa tgccaaagct gctggcaaag gcaagctgga cgtccagttc
     4321 tcaggactca ccaaggggga tgcagtgcga gatgtggaca tcatcgacca ccatgacaac
     4381 acctacacag tcaagtacac gcctgtccag cagggtccag taggcgtcaa tgtcacttat
     4441 ggaggggatc ccatccctaa gagccctttc tcagtggcag tatctccaag cctggacctc
     4501 agcaagatca aggtgtctgg cctgggagag aaggtggacg ttggcaaaga ccaggagttc
     4561 acagtcaaat caaagggtgc tggtggtcaa ggcaaagtgg catccaagat tgtgggcccc
     4621 tcgggtgcag cggtgccctg caaggtggag ccaggcctgg gggctgacaa cagtgtggtg
     4681 cgcttcctgc cccgtgagga agggccctat gaggtggagg tgacctatga cggcgtgccc
     4741 gtgcctggca gcccctttcc tctggaagct gtggccccca ccaagcctag caaggtgaag
     4801 gcgtttgggc cggggctgca gggaggcagt gcgggctccc ccgcccgctt caccatcgac
     4861 accaagggcg ccggcacagg tggcctgggc ctgacggtgg agggcccctg tgaggcgcag
     4921 ctcgagtgct tggacaatgg ggatggcaca tgttccgtgt cctacgtgcc caccgagccc
     4981 ggggactaca acatcaacat cctcttcgct gacacccaca tccctggctc cccattcaag
     5041 gcccacgtgg ttccctgctt tgacgcatcc aaagtcaagt gctcaggccc cgggctggag
     5101 cgggccaccg ctggggaggt gggccaattc caagtggact gctcgagcgc gggcagcgcg
     5161 gagctgacca ttgagatctg ctcggaggcg gggcttccgg ccgaggtgta catccaggac
     5221 cacggtgatg gcacgcacac cattacctac attcccctct gccccggggc ctacaccgtc
     5281 accatcaagt acggcggcca gcccgtgccc aacttcccca gcaagctgca ggtggaacct
     5341 gcggtggaca cttccggtgt ccagtgctat gggcctggta ttgagggcca gggtgtcttc
     5401 cgtgaggcca ccactgagtt cagtgtggac gcccgggctc tgacacagac cggagggccg
     5461 cacgtcaagg cccgtgtggc caacccctca ggcaacctga cggagaccta cgttcaggac
     5521 cgtggcgatg gcatgtacaa agtggagtac acgccttacg aggagggact gcactccgtg
     5581 gacgtgacct atgacggcag tcccgtgccc agcagcccct tccaggtgcc cgtgaccgag
     5641 ggctgcgacc cctcccgggt gcgtgtccac gggccaggca tccaaagtgg caccaccaac
     5701 aagcccaaca agttcactgt ggagaccagg ggagctggca cgggcggcct gggcctggct
     5761 gtagagggcc cctccgaggc caagatgtcc tgcatggata acaaggacgg cagctgctcg
     5821 gtcgagtaca tcccttatga ggctggcacc tacagcctca acgtcaccta tggtggccat
     5881 caagtgccag gcagtccttt caaggtccct gtgcatgatg tgacagatgc gtccaaggtc
     5941 aagtgctctg ggcccggcct gagcccaggc atggttcgtg ccaacctccc tcagtccttc
     6001 caggtggaca caagcaaggc tggtgtggcc ccattgcagg tcaaagtgca agggcccaaa
     6061 ggcctggtgg agccagtgga cgtggtagac aacgctgatg gcacccagac cgtcaattat
     6121 gtgcccagcc gagaagggcc ctacagcatc tcagtactgt atggagatga agaggtaccc
     6181 cggagcccct tcaaggtcaa ggtgctgcct actcatgatg ccagcaaggt gaaggccagt
     6241 ggccccgggc tcaacaccac tggcgtgcct gccagcctgc ccgtggagtt caccatcgat
     6301 gcaaaggacg ccggggaggg cctgctggct gtccagatca cggatcccga aggcaagccg
     6361 aagaagacac acatccaaga caaccatgac ggcacgtata cagtggccta cgtgccagac
     6421 gtgacaggtc gctacaccat cctcatcaag tacggtggtg acgagatccc cttctccccg
     6481 taccgcgtgc gtgccgtgcc caccggggac gccagcaagt gcactgtcac aggtgctggc
     6541 atcggcccca ccattcagat tggggaggag acggtgatca ctgtggacac taaggcggca
     6601 ggcaaaggca aagtgacgtg caccgtgtgc acgcctgatg gctcagaggt ggatgtggac
     6661 gtggtggaga atgaggacgg cactttcgac atcttctaca cggcccccca gccgggcaaa
     6721 tacgtcatct gtgtgcgctt tggtggcgag cacgtgccca acagcccctt ccaagtgacg
     6781 gctctggctg gggaccagcc ctcggtgcag ccccctctac ggtctcagca gctggcccca
     6841 cagtacacct acgcccaggg cggccagcag acttgggccc cggagaggcc cctggtgggt
     6901 gtcaatgggc tggatgtgac cagcctgagg ccctttgacc ttgtcatccc cttcaccatc
     6961 aagaagggcg agatcacagg ggaggttcgg atgccctcag gcaaggtggc gcagcccacc
     7021 atcactgaca acaaagacgg caccgtgacc gtgcggtatg cacccagcga ggctggcctg
     7081 cacgagatgg acatccgcta tgacaacatg cacatcccag gaagcccctt gcagttctat
     7141 gtggattacg tcaactgtgg ccatgtcact gcctatgggc ctggcctcac ccatggagta
     7201 gtgaacaagc ctgccacctt caccgtcaac accaaggatg caggagaggg gggcctgtct
     7261 ctggccattg agggcccgtc caaagcagaa atcagctgca ctgacaacca ggatgggaca
     7321 tgcagcgtgt cctacctgcc tgtgctgccg ggggactaca gcattctagt caagtacaat
     7381 gaacagcacg tcccaggcag ccccttcact gcccgggtca caggtgacga ctccatgcgt
     7441 atgtcccacc taaaggtcgg ctctgctgcc gacatcccca tcaacatctc agagacggat
     7501 ctcagcctgc tgacggccac tgtggtcccg ccctcgggcc gggaggagcc ctgtttgctg
     7561 aagcggctgc gtaatggcca cgtggggatt tcattcgtgc ccaaggagac gggggagcac
     7621 ctggtgcatg tgaagaaaaa tggccagcac gtggccagca gccccatccc ggtggtgatc
     7681 agccagtcgg aaattgggga tgccagtcgt gttcgggtct ctggtcaggg ccttcacgaa
     7741 ggccacacct ttgagcctgc agagtttatc attgataccc gcgatgcagg ctatggtggg
     7801 ctcagcctgt ccattgaggg ccccagcaag gtggacatca acacagagga cctggaggac
     7861 gggacgtgca gggtcaccta ctgccccaca gagccaggca actacatcat caacatcaag
     7921 tttgccgacc agcacgtgcc tggcagcccc ttctctgtga aggtgacagg cgagggccgg
     7981 gtgaaagaga gcatcacccg caggcgtcgg gctccttcag tggccaacgt tggtagtcat
     8041 tgtgacctca gcctgaaaat ccctgaaatt agcatccagg atatgacagc ccaggtgacc
     8101 agcccatcgg gcaagaccca tgaggccgag atcgtggaag gggagaacca cacctactgc
     8161 atccgctttg ttcccgctga gatgggcaca cacacagtca gcgtcaagta caagggccag
     8221 cacgtgcctg ggagcccctt ccagttcacc gtggggcccc taggggaagg gggagcccac
     8281 aaggtccgag ctgggggccc tggcctggag agagctgaag ctggagtgcc agccgaattc
     8341 agtatctgga cccgggaagc tggtgctgga ggcctggcca ttgctgtcga gggccccagc
     8401 aaggctgaga tctcttttga ggaccgcaag gacggctcct gtggtgtggc ttatgtggtc
     8461 caggagccag gtgactacga agtctcagtc aagttcaacg aggaacacat tcccgacagc
     8521 cccttcgtgg tgcctgtggc ttctccgtct ggcgacgccc gccgcctcac tgtttctagc
     8581 cttcaggagt cagggctaaa ggtcaaccag ccagcctctt ttgcagtcag cctgaacggg
     8641 gccaaggggg cgatcgatgc caaggtgcac agcccctcag gagccctgga ggagtgctat
     8701 gtcacagaaa ttgaccaaga taagtatgct gtgcgcttca tccctcggga gaatggcgtt
     8761 tacctgattg acgtcaagtt caacggtacc cacatccctg gaagcccctt caagatccga
     8821 gttggggagc ctgggcatgg aggggaccca ggcttggtgt ctgcttacgg agcaggtctg
     8881 gaaggcggtg tcacagggaa cccagctgag ttcgtcgtga acacgagcaa tgcgggagct
     8941 ggtgccctgt cggtgaccat tgacggcccc tccaaggtga agatggattg ccaggagtgc
     9001 cctgagggct accgcgtcac ctataccccc atggcacctg gcagctacct catctccatc
     9061 aagtacggcg gcccctacca cattgggggc agccccttca aggccaaagt cacaggcccc
     9121 cgtctcgtca gcaaccacag cctccacgag acatcatcag tgtttgtaga ctctctgacc
     9181 aaggccacct gtgcccccca gcatggggcc ccgggtcctg ggcctgctga cgccagcaag
     9241 gtggtggcca agggcctggg gctgagcaag gcctacgtag gccagaagag cagcttcaca
     9301 gtagactgca gcaaagcagg caacaacatg ctgctggtgg gggttcatgg cccaaggacc
     9361 ccctgcgagg agatcctggt gaagcacgtg ggcagccggc tctacagcgt gtcctacctg
     9421 ctcaaggaca agggggagta cacactggtg gtcaaatggg ggcacgagca catcccaggc
     9481 agcccctacc gcgttgtggt gccctaaaag cttcgaattc tgcagtcgac ggtaccgcgg
     9541 gcccgggatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
     9601 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     9661 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9721 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9781 tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
     9841 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     9901 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     9961 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    10021 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
    10081 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    10141 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
    10201 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
    10261 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
    10321 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
    10381 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
    10441 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    10501 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    10561 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
    10621 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    10681 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    10741 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
    10801 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
    10861 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
    10921 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
    10981 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
    11041 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
    11101 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
    11161 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
    11221 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
    11281 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
    11341 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
    11401 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
    11461 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
    11521 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
    11581 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
    11641 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
    11701 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc
    11761 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
    11821 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
    11881 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
    11941 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
    12001 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
    12061 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    12121 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    12181 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    12241 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    12301 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    12361 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    12421 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    12481 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    12541 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    12601 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    12661 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    12721 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    12781 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    12841 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag
    12901 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccg
//
