LOCUS       sequence-18169-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     Integrated Genomics
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACT2.2
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..52
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             32..58
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     terminator      488..675
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(543..567)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(696..729)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(807..840)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(884..917)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acccaaaaaa gagatctcta tggcttaccc atacgatgtt ccagattacg ctagcttggg
       61 tggtcatatg gccatggagg ccccggggat ccgaattcga gctcgagaga tctatgaatc
      121 gtagatactg aaaaaccccg caagttcact tcaactgtgc atcgtgcacc atctcaattt
      181 ctttcattta tacatcgttt tgccttcttt tatgtaacta tactcctcta agtttcaatc
      241 ttggccatgt aacctctgat ctatagaatt ttttaaatga ctagaattaa tgcccatctt
      301 ttttttggac ctaaattctt catgaaaata tattacgagg gcttattcag aagctttgga
      361 cttcttcgcc agaggtttgg tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc
      421 agaaatttac gaaaagatgg aaaagggtca aatcgttggt agatacgttg ttgacacttc
      481 taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat
      541 aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag
      601 taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga
      661 ccacacctct accggcatgc cggtcgaggg acctaataac ttcgtatagc atacattata
      721 cgaagttata ttaagggttc cggatcgcgg ccgctcgacc tgcagccaag ctagcttggc
      781 tggacgtaaa ctcctcttca gacctaataa cttcgtatag catacattat acgaagttat
      841 attaagggtt attgaatatg atcggaattg gtcgagggac ctaataactt cgtatagcat
      901 acattatacg aagttatatt
//
