LOCUS       sequence-181694-        1681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a fusion of human histone H2B to fluorescent protein 
            mCherry in the pCS2+ expression vector. It has the SP6 RNA 
            polymerase site for in vitro transcription..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Wan Y, Wei Z, Looger LL, Koyama M, Druckmann S, Keller PJ
  TITLE     Single-Cell Reconstruction of Emerging Population Activity in an 
            Entire Developing Circuit.
  JOURNAL   Cell. 2019 Oct 3;179(2):355-372.e23. doi: 
            10.1016/j.cell.2019.08.039. Epub 2019 Sep 26.
  PUBMED    31564455
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.039"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "355-372.e23"
COMMENT     Sequence Label: pCS2+ H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..1681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        110..128
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     110..127
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             179..556
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      569..578
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             575..1282
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(720..738)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     988..1007
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1182..1205
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1315..1449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1339..1358)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1393..1412
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1562..1582)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1562..1580)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1601..1617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1601..1617)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1614..1636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1625..1641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1649..1655,1656..1673,1674..1679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggcgttccat tgacgtaaat gggcggtagg cgtgcctaat gggaggtcta tataagcaat
       61 gctcgtttag ggaaccgcca ttctgcctgg ggacgtcgga gcaagcttga tttaggtgac
      121 actatagaat acaagctact tgttcttttt gcaggatccc atcgacggta ccgccaccat
      181 gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc tccaagaagg cggtgactaa
      241 ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc aaggagagct attccatcta
      301 tgtgtacaag gttctgaagc aggtccaccc tgacaccggc atttcgtcca aggccatggg
      361 catcatgaat tcgtttgtga acgacatttt cgagcgcatc gcaggtgagg cttcccgcct
      421 ggcgcattac aacaagcgct cgaccatcac ctccagggag atccagacgg ccgtgcgcct
      481 gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag ggtactaagg ccatcaccaa
      541 gtacaccagc gctaaggatc caccggtcgc caccatggtg agcaagggcg aggaggacaa
      601 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      661 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      721 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccctca
      781 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      841 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      901 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      961 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     1021 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     1081 gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct acaaggccaa
     1141 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagctggaca tcacctccca
     1201 caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc actccaccgg
     1261 cggcatggac gagctgtaca agttatctag aactatagtg agtcgtatta cgtagatcca
     1321 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     1381 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     1441 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     1501 gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt
     1561 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     1621 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     1681 c
//
