LOCUS       sequence-181707-       14137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This is a S. elongatus (PCC7942) shuttle vector used for 
            recombination of the pylRS orthogonal translation system to genomic 
            neutral site 2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14137)
  AUTHORS   Chemla Y, Friedman M, Heltberg M, Bakhrat A, Nagar E, Schwarz R, 
            Jensen MH, Alfonta L
  TITLE     Expanding the Genetic Code of a Photoautotrophic Organism.
  JOURNAL   Biochemistry. 2017 Apr 12. doi: 10.1021/acs.biochem.7b00131.
  PUBMED    28394580
REFERENCE   2  (bases 1 to 14137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2017 Apr 12. doi: 10.1021/acs.biochem.7b00131."
COMMENT     Sequence Label: gCOTS-pyl
FEATURES             Location/Qualifiers
     source          1..14137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        329..433
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(434..502,503..1294)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1465..2053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1954..1973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2207..2224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2239..2379
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2305..2324)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2465..2487
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2481..2672)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     4134..4153
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(5217..5876)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5791..5810
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5877..5979)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        6182..6286
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6864..6950
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7042..7069
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        10614..10829
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10830..11633
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPAAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ccaaagtcac caacagaccc agttcattga gcatgtctgc aaagacgcga ttagtgatgc
       61 gttcgctatc aacaagcaca ccatcacagt cgaaaatcac cagctgaaac ggtgaagttt
      121 gcattgtttt taagcacgag ccatcaacag taagagcgat cgcgctggga cgatgtaatc
      181 gcgccgtagg cagggttttg cctcatccgg cagatgacaa gctaattctt gaagacgaaa
      241 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
      301 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      361 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      421 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      481 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      541 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      601 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      661 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
      721 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      781 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      841 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      901 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      961 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1021 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1081 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1141 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     1201 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     1261 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     1321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1861 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2101 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2161 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     2221 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     2281 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     2341 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     2401 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     2461 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     2521 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
     2581 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     2641 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     2701 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     2761 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     2821 cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt
     2881 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
     2941 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
     3001 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     3061 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     3121 acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg
     3181 ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt
     3241 ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc
     3301 cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc
     3361 agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg
     3421 cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg
     3481 cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct
     3541 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc
     3601 agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga
     3661 taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga
     3721 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
     3781 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
     3841 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
     3901 tgactgggtt gaaggctctc aagggcatcg gtcgatcgac gctctccctt atgcgactcc
     3961 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     4021 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     4081 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     4141 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     4201 ccggcgtaga ggatccacgc attttaattc tggatgaggc gacttcggcg cttgattccg
     4261 agtccgaaac cttggtgcaa gaggccctag aacgggtgat gcagggacgg acggtcttta
     4321 tcattgctca ccgtctggct acggttcgta atgccagccg catcttggtg atggagcgcg
     4381 gccagattgt tgaggcaggc aatcacgatg cgctgttggc agaggctggc cgctatgcgc
     4441 gatactacgc acagcaattt cgtgcctgat caggattaat gaaacggacg ccccagactg
     4501 aattgccgga ctgggaagcg atcgcggatt tggatgcgat cgtggtcgat aaacgagctc
     4561 gtaagcgggc cacggcagcg aaagggcgac ggcgcgatcg ccggtatgga aaacgcttgc
     4621 tgcagcatca gatcgatgcg atcgccgaag actgtgacct agatgaggaa gcatgagcga
     4681 actgggcttg agtctgacgg cgatcgcgat ttttaccacg acggcattag ctttggtggg
     4741 accaatgctg ggtagttctc cgctgctacc ggcgggattg ggttttagcc tcttggtgct
     4801 gttcagtctg gatgcggtga cttggcaggg gcggggtgcc acgttactgc tcgatggcat
     4861 tcagcagcga tcgcccgaat atcgtcagcg gattttgcat cacgaagcgg gtcactactt
     4921 ggtagcaacc gcgctggggt taccagatcc gtcgatcata tcgtcaatta ttacctccac
     4981 ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca cactgcttcc
     5041 ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc
     5101 tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca
     5161 cttattcagg cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac
     5221 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     5281 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     5341 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     5401 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     5461 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     5521 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     5581 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     5641 tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     5701 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     5761 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     5821 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     5881 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     5941 tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag
     6001 ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga
     6061 tcttccgtca caggtattta ttcgaagacg aaagggcctc gtgatacgcc tatttttata
     6121 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     6181 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6241 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6301 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6361 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6421 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6481 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg tgacggatct
     6541 aagcttgctt ctttgctgac gagtggcgga cgggtgagta atgtctggga aactgcctga
     6601 tggaggggga taactactgg aaacggtagc taataccgca taacgtcgca agaccaaaga
     6661 gggggacctt cgggcctctt gccatcggat gtgcccagat gggattagct agtaggtggg
     6721 gtaacggctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc
     6781 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
     6841 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     6901 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
     6961 cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa
     7021 ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
     7081 aaactccgga tccggccgga tccggccaag cttgctagct cgagcccggg cctgtctagg
     7141 cagtagtaga caactcagtt gctgacctca gagactgctt gacaaaattt tctatcggac
     7201 taaagtaatt aattgtgcgg ttcgcacttg ccaggaaacc tgatcatgta gatcgaatgg
     7261 actctaaatc cgttcagccg ggttagattc ccggggtttc cgccattctt tcttacccct
     7321 gtcatcaatg gtgatagggg ttcgtttttt gttatctgtg actattgccc acgcagtagg
     7381 aatctggctg tccatggcag tgggcggaat ctcatccgcc ggtatcgata agcttgaaac
     7441 aaaacgttta aaactttatg taataacaaa tttaaatatg taagttaaga actttcaaag
     7501 aataacttat gccatttctt gatatattgt gagacaagtt acaaattacg tggtgtgcaa
     7561 ttttttcatc ttgcgctgat tactctacta aatatccgtc aagtaaattg gctcttagct
     7621 cgtctcctgt caataaagga ggtcggcaag agtgcagaag cgggaatgtg tgaaaactaa
     7681 cccaattcat taaatacccc gaaatatagg ggaatcatct catactttcc gtaaaccgcg
     7741 aaggtcgtga agggataaaa gcaatttagt gggtgagaag aacagataaa aaagaatttt
     7801 ttaactatgg caagaggaaa aagtaaaagc gttatcgaat tcctgcagcc cgggggatcc
     7861 tctagattta agaaggagat atacatatgg ataaaaaacc actaaacact ctgatctctg
     7921 ctactggtct gtggatgagt cgtaccggaa ccattcataa aatcaaacac cacgaggtta
     7981 gccgttcgaa aatctatatt gagatggcgt gtggcgatca tctggttgtg aacaatagcc
     8041 gctcttctcg tacagcacgt gcactgcgtc accacaaata tcgtaaaacc tgtaaacgtt
     8101 gccgtgtgtc cgatgaggat ctgaacaaat tcctgacaaa agccaatgag gaccaaacaa
     8161 gcgtgaaagt gaaagtcgtt agcgctccta cccgtactaa aaaagcaatg ccgaaatccg
     8221 ttgctcgtgc ccctaaacca ctggaaaaca ctgaagcagc acaggcacag ccgtctggaa
     8281 gcaaattctc tccggccatt cctgtttcta cccaggagtc cgtttctgtt ccagcaagtg
     8341 tgagcaccag cattagcagt attagcaccg gtgccaccgc tagcgccctg gttaaaggca
     8401 ataccaatcc gattacaagc atgtctgccc cggttcaagc atcagctcca gcactgacaa
     8461 aatcccaaac cgatcgtctg gaggttctgc tgaatccgaa agacgaaatc agcctgaatt
     8521 ccggcaaacc gtttcgtgaa ctggagagcg aactgctgtc acgtcgtaaa aaagacctgc
     8581 aacaaatcta tgccgaagaa cgtgagaact atctggggaa actggaacgt gaaatcaccc
     8641 gctttttcgt ggatcgtggc tttctggaga tcaaatcccc gattctgatt cctctggagt
     8701 atatcgagcg tatgggcatc gacaatgata ccgaactgag caaacaaatt ttccgtgtgg
     8761 ataaaaactt ctgtctgcgc cctatgctgg caccaaatct gtataactat ctgcgcaaac
     8821 tggaccgtgc cctgcctgat cctatcaaaa tcttcgagat cggcccgtgt tatcgtaaag
     8881 agtccgacgg taaagaacat ctggaggagt ttaccatgct gaacttttgc caaatgggtt
     8941 caggttgtac tcgtgagaac ctggaaagca tcatcaccga ttttctgaac cacctgggca
     9001 ttgacttcaa aattgtgggc gacagctgta tggtgtatgg cgacaccctg gatgtcatgc
     9061 acggcgacct ggaactgtct agtgccgttg ttggaccaat tccgctggac cgtgagtggg
     9121 gtatcgacaa accgtggatc ggagcaggat tcggtctgga acgcctgctg aaagtgaaac
     9181 acgacttcaa aaacatcaaa cgtgccgccc gttctgaatc gtattataac gggatttcta
     9241 ccaacctgta agtcgacaga tctgcgaata ccgcttccac aaacattgct caaaagtatc
     9301 tctttgctat atatctctgt gctatatccc tatataacct acccatccac ctttcgctcc
     9361 ttgaacttgc atctaaactc gacctctaca ttttttatgt ttatctctag tattactctt
     9421 tagacaaaaa aattgtagta agaactattc atagagtgaa tcgaaaacaa tacgaaaatg
     9481 taaacatttc ctatacgtag tatatagaga caaaatagaa gaaaccgttc ataattttct
     9541 gaccaatgaa gaatcatcaa cgctatcact ttctgttcac aaagtatgcg caatccacat
     9601 cggtatagaa tataatcggg gatgccttta tcttgaaaaa atgcacccgc agcttcgcta
     9661 gtaatcagta aacgcgggaa gtggagtcag gcttttttta tggaagagaa aatagacacc
     9721 aaagtagcct tcttctaacc ttaacggacc tacagtgcaa aaagttatca agagactgca
     9781 ttatagagcg cacaaaggag aaaaaaagta atctaagatg ctttgttaga aaaatagcgc
     9841 tctcgggatg catttttgta gaacaaaaaa gaagtataga ttctttgttg gtaaaatagc
     9901 gctctcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt
     9961 agcgctctcg cgctgcattt ttgttctaca aaatgaagca cagatgcttc gttaacaaag
    10021 atatgctatt gaagtgcaag atggaaacgc agaaaatgaa ccggggatgc gacgtgcaag
    10081 attacctatg caatagatgc aatagtctct ccaggaaccg aaatacatac attgtcttcc
    10141 gtaaagcgct agactatata ttattataca ggttcaaata tactatctgt ttcagggaaa
    10201 actcccaggt tcggatgttc aaaattcaat gatgggtaac gagtacgatc gtaaatctgt
    10261 aaaacagttt gtcggatatt aggctgtatc tcctcaaagc gtattcgaat atcattgaga
    10321 agctgcagcg tcacatcgga taataatgtt ggcagccatt gtagaagtgc cttttgcatt
    10381 tctagtctct ttctcggtct agctagtttt actacatcgc gaagatagaa tcttagatca
    10441 cactgccttt gctgagctgg atcaatagag taacaaaaga gtggtaaggc ttcgttaaag
    10501 gacaaggacc tgagcggaag tgtatcgtac agtagacgga gtatactagg tatagtctat
    10561 agtccgtgga attaattctc atgtttgaca gcttatcatc gataagctag cttttcaatt
    10621 catcattttt tttttattct tttttttgat ttcggtttcc ttgaaatttt tttgattcgg
    10681 taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac
    10741 gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa
    10801 caaaaacctg caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg
    10861 ctactcatcc tagtcctgct gctgccaagc tatttaatat catgcacgaa aagcaaacaa
    10921 acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat
    10981 taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg
    11041 agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag
    11101 acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca
    11161 gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta
    11221 gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag
    11281 cagaattgtc atgcaagggc tccctatcta ctggagagta tactaagggt actgttgaca
    11341 ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagggac atgggtggaa
    11401 gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag
    11461 acgcattggg tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta
    11521 ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt
    11581 acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac tagatctttg
    11641 tcttattggt tcgccatact ttatttattt actctgccat tatatgaaag ataaaaaaat
    11701 ctcattttct ggagataaaa gcctttatat cataaccgga ggcggctgga aaagttacga
    11761 aaaagaatct ctgaaacgtg atgatttcaa tcatctttta tttgatactt tcaatctcag
    11821 tgatattagt cagatccgag atatatttaa tcaagttgaa ctcaacactt gtttctttga
    11881 ggatgaaatg cagcgtaaac atgttccgcc gtgggtatat gcgcgagcgc ttgatcctga
    11941 aacgttgaaa cctgtacctg atggaacgcc ggggttgatg agttatatgg atgcgtcagc
    12001 aaccagttat ccagcattta ttgttaccga tgatgtcggg ataattagca gagaatatgg
    12061 taagtatccc ggcgtgctcg ttgaaatttt acgtcgcgtc aatacgagga cgcagaaagg
    12121 gtgtgcttta agcttaaccg aagcgtttga tagttgatat cctttgccta attgtaagtg
    12181 gaatgcttgc gttatataaa tctgaatgac atctacactt tacaaaattc tccaaaacat
    12241 ccacatttgg gtacttgata gaggtttatg gggttggctt aacattgttc tcattgttat
    12301 tattggctca aagcaaaagg agataacatg aaaaaattgg cagttatgct tgcattggga
    12361 atgattagct ttggtgcaat ggcagttgat gggtataaag atgcaaagtt tggcatgaca
    12421 gaagaagagt ttctttcgaa gaggttatgt gattttgaaa aatttgaggg agattctcga
    12481 atagaagaag tatcacttta ttcatgttct gacttttcgt ttgctaacaa aaagcgtgaa
    12541 gcaatggcat tttttttaaa tgggaaattt aaaagattag agattaatat tggcagactt
    12601 gtgaagccag taagcaaatc gttaacgaaa aagtacggag atggatcatc gtatccatca
    12661 aaagaagaat ttgagaacgc gctaaaatac aatggaacta tgtctatagg ttatgataat
    12721 aatacggtat tagttgatat acatataata tgtggcaaag aaggcataga aaccagtcaa
    12781 ctgatttata cgagtccaga tgtttatacg ctcccagatt tcggagaaaa aatccaggaa
    12841 ttaaagggat taaaggaatt cgagctcggt acccatcggt tacccgtgac gggctacacc
    12901 ctctcagcgt gggaagcgct gcgccaagga caacctggtc gcgggggtgt gcagttccaa
    12961 gcagctgcgc tagaagccga agccgcacag gggcaactca gtcagcgatc gctggaacag
    13021 tggtgtcagg tgttgatggc cggtgcagcg gcagagcaac tggtctacgg caacgtggaa
    13081 gggggagctg acgatcgcgc ccagtggaaa caactgtggc ggcaactcga tcgcaatcct
    13141 gccgaagcgg atttacgcag tcgctgggga ttgttacggg cgaagacttt actggagcaa
    13201 caacgtcccg cctacgatgc tttggtggcg gcgatggctg cagaggccag cattgaagac
    13261 tgcaatcaag cgatcgccac tgcttgggta gaagaacctg cgatcgccgc ttagtgaaga
    13321 gtccagaaga ttcccctccc ctctcgccca atggacaagg gaaatgtgat tcagcatgag
    13381 tcaagtcccc agtgcatgga tgggtgtgca atgacggaca gaggctgttg actctcagtt
    13441 atccctgtaa gccaaatcgt ccgaaaatca ccagctgaaa cggttagggt tgcattgttt
    13501 ttaagcacga gccatcaaca gtaagagcga tcgcgcagga acggtttagt gtgtagttgc
    13561 ggcgcgagca ggggttaggg attgggcaat gctgtggagt tatcttttgg cgacgattgg
    13621 agcagactgg gcagcagtcg catatcgtca aaaatgagat gggcaccggc ttcttgcaga
    13681 cgccaagcgg gcatggaacc cgcgtagcca aacacttgca ttcctgccgc cacgcctgcc
    13741 gctactccca agggggtgtc ttcgatcaca gcgcaggccg taggattaac cccgaagcga
    13801 tcggcggcca acaaaaagac atcgggaaat ggcttgccgc gaggtacttc agtcacgctg
    13861 aagattcgtc cctcaaatcg tggccagagc ttcgtcaggc tcagtgtggt tcgcatcttt
    13921 tgatgatcac cactggacgc aacacagtag ggcaattcaa gagcatccaa agcctcttca
    13981 accccaggaa cggcttgtag atgcgtttct aacgcgatac gggtacggcg ttgatagtgc
    14041 tgaacaaagt cagggggtgg aggattgcct aaccgtcgct caattagttt gagacagtca
    14101 gccatggaat gacccacaaa ctgctcaaac atgtcat
//
