LOCUS       sequence-181719-        6033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 490 / 553, Emission: 507 / 
            573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: Dendra2-Gamma-Tubulin-17
FEATURES             Location/Qualifiers
     source          1..6033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1947..2639
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     polyA_signal    2761..2882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2798..2817)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2852..2871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2889..3344)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3026..3047)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3238..3257
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3371..3475
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3477..3834
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3477..3668
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3500..3520)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3685..3820
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3869..4663
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3869..4663
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3923..3942)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4533..4552
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4851..4870)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4895..4942
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5271..5859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5760..5779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      541 ccatgccgag ggaaatcatc accctacagt tgggccagtg cggcaatcag attgggttcg
      601 agttctggaa acagctgtgc gccgagcatg gtatcagccc cgagggcatc gtggaggagt
      661 tcgccaccga gggcactgac cacaaggacg tctttttcta ccaggcagac gatgagcact
      721 acatcccccg ggccgtgctg ctggacttgg aaccccgggt gatccactcc atcctcaact
      781 ccccctatgc caagctctac aacccagaga acatctacct gtcggaacat ggaggaggag
      841 ctggcaacaa ctgggccagc ggattctccc agggagaaaa gatccatgag gacatttttg
      901 acatcataga ccgggaggca gatggtagtg acagtctaga gggctttgtg ctgtgtcact
      961 ccattgctgg ggggacaggc tctggactgg gttcctacct cttagaacgg ctgaatgaca
     1021 ggtatcctaa gaagctggtg cagacatact cagtgtttcc caaccaggac gagatgagcg
     1081 atgtggtggt ccagccttac aattcactcc tcacactcaa gaggctgacg cagaatgcag
     1141 actgtgtggt ggtgctggac aacacagccc tgaaccggat tgccacagac cgcctgcaca
     1201 tccagaaccc atccttctcc cagatcaacc agctggtgtc taccatcatg tcagccagca
     1261 ccaccaccct gcgctaccct ggctacatga acaatgacct catcggcctc atcgcctcgc
     1321 tcattcccac cccacggctc cacttcctca tgaccggcta cacccctctc actacggacc
     1381 agtcagtggc cagcgtgagg aagaccacgg tcctggatgt catgaggcgg ctgctgcagc
     1441 ccaagaacgt gatggtgtcc acaggccgag accgccagac caaccactgc tacatcgcca
     1501 tcctcaacat catccaggga gaggtggacc ccacccaggt ccacaagagc ttgcagagga
     1561 tccgggaacg caagttggcc aacttcatcc cgtggggccc cgccagcatc caggtggccc
     1621 tgtcgaggaa gtctccctac ctgccctcgg cccaccgggt cagcgggctc atgatggcca
     1681 accacaccag catctcctcg ctcttcgaga gaacctgtcg ccagtatgac aagctgcgta
     1741 agcgggaggc cttcctggag cagttccgca aggaggacat gttcaaggac aactttgatg
     1801 agatggacac atccagggag attgtgcagc agctcatcga tgagtaccat gcggccacac
     1861 ggccagacta catctcctgg ggcactcagg agcagcgaat tctgcagtcg acggtaccgc
     1921 gggcccggga tccaccggtc gccaccatga acaccccggg aattaacctg atcaaggagg
     1981 acatgcgcgt gaaggtgcac atggagggca acgtgaacgg ccacgccttc gtgatcgagg
     2041 gcgagggcaa gggcaagccc tacgagggca cccagaccgc caacctgacc gtgaaggagg
     2101 gcgcccccct gcccttcagc tacgacatcc tgaccaccgc cgtgcactac ggcaaccggg
     2161 tgttcaccaa gtaccccgag gacatccccg actacttcaa gcagagcttc cccgagggct
     2221 acagctggga gcgcaccatg accttcgagg acaagggcat ctgcaccatc cgcagcgaca
     2281 tcagcctgga gggcgactgc ttcttccaga acgtgcgctt caagggcacc aacttccccc
     2341 ccaacggccc cgtgatgcag aagaagaccc tgaagtggga gcccagcacc gagaagctgc
     2401 acgtgcgcga cggcctgctg gtgggcaaca tcaacatggc cctgctgctg gagggcggcg
     2461 gccactacct gtgcgacttc aagaccacct acaaggccaa gaaggtggtg cagctgcccg
     2521 acgcccactt cgtggaccac cgcatcgaga tcctgggcaa cgacagcgac tacaacaagg
     2581 tgaagctgta cgagcacgcc gtggcccgct acagccccct gcccagccag gtgtggtaag
     2641 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     2701 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     2761 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     2821 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     2881 taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     2941 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     3001 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     3061 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     3121 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     3181 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     3241 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     3301 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     3361 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3421 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
     3481 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     3541 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     3601 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3661 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     3721 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     3781 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
     3841 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     3901 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     3961 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     4021 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     4081 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     4141 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     4201 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     4261 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     4321 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     4381 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     4441 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     4501 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     4561 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     4621 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     4681 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     4741 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     4801 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
     4861 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     4921 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     4981 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     5041 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     5101 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
     5161 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5221 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5281 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5341 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5401 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5461 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5521 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5581 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5641 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5701 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5761 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5821 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5881 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5941 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
     6001 gtcattagtt catagcccat atatggagtt ccg
//
