LOCUS       sequence-18172-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  TITLE     Integrated Genomics
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACT2.2gtwy
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..62
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             42..68
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    99..223
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(248..253,254..271,272..278)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(398..417)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccccaccaaa cccaaaaaaa gagatctcta tggcttaccc atacgatgtt ccagattacg
       61 ctagcttggg tggtcatatg gccatggagg ccccatcaac aagtttgtac aaaaaagctg
      121 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
      181 agactacata atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac
      241 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc
      301 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
      361 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
      421 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
      481 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
      541 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
      601 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
      661 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
      721 cctggcctat t
//
