LOCUS       sequence-181722-        7066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB2 with split pyrG marker for homologous integration with linker AB
            (negative control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8
FEATURES             Location/Qualifiers
     source          1..7066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2697..2714)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2868..3456)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2948..2967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3682..4473,4474..4542))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4305..4324
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4543..4647)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atctcagaga ctttggcctc aatctagaat ctatctagtt gtcaactaga ctgtggtatc
       61 attgtctttt attttcctag tcctggaact agcttctaac tagtctccct aatatgtggc
      121 tgtcttgttt tttttttttg tttccctacc cggatatcta gtccccttct aggttctgtt
      181 aacctctcgg gctctgattt agtttaacgc aaacctgaga ttagtttcta actagtctct
      241 aggttttcta tccaccttta attgtaataa taaatacaag caacgtttat acgtcaaaag
      301 catttataaa cttttaccct aaagtagctt gcttgtgtgt ttagtttata attagtctct
      361 tattaatttg atgtaggtaa gcccgccaca aatatatatt tttaacaaga taccgtggaa
      421 aaacttcgtg ctatcacaaa acagtataca aaaaataagc ttgtcgacga attcagatat
      481 cacgaattct ggatccgata cgtaacgcgt ctgcagcata agcgtaggtc aattgcgact
      541 tggaggttaa tgttcacacc agtcgtgaaa accacaaagt cctcctcatc cgaaggagag
      601 ctgacttccg actgcacctc acccaacgcg cgcgtcgaca cgaatcccat aacgaagttc
      661 ttgtatttcc gggcataatc gaccgaggaa gtagtgtact ggccggtagc caaggagccc
      721 ttagaggtca tctctgccaa gatcaacaga ccgcgttcgg ggccgtaggc gaagtccggt
      781 gcagacgccg tctgagcgag agcctcgacg ataccctcac cagggagaat gctgcagttg
      841 atgatgtggg cccattccga gatacggagg gtaccgccgt ggtattgctt ctggaccgtg
      901 ttgccgatgt caatgaactt gcggtcctcg aagatgagaa agttgtgctt ctgcgcgaga
      961 gccttaagtc cctcaatagt ctcgttgctg aaatcagaga ggatatcgat gtgggttttg
     1021 atcacggcaa tgtagggacc gagacggtca gcaagatcta gtagctcctt agtggtggta
     1081 acgtcagccg agacagtcac attggttttc ttggcctcgg caatctcgaa cagcctcttc
     1141 gccagagcat tgggatgctt gctggcacgg gcagtgtagg tgagctggga cttgctgctc
     1201 atgtgtcctg gtgggtgggt tgcaatgacg gcagacaagg caaggacgtt tggagatgag
     1261 ggagtttgga ggttgaggtg agtttgaagc cgcttcggcc cgtggtcgcc tgcccttagt
     1321 cagcgccggg gattgattgg ttgcggagct tctccggctg cccgcagtga cctggtccgc
     1381 agacctactc gattgggtgt ccggcttagg gcttagggtc tataatctgc tccagtggtc
     1441 atctgcggtc tgccgtagat aaggaacaca tgccccggcc gagcaatggc cgcgttcagc
     1501 ccgggatcag ccggtgctaa actgcgagag cggacgctag tgtcggttca ttgcgaacga
     1561 acaaatgccg gcgccatatg ttcccccggg ctaaaatgaa ttatcataca tcacttaata
     1621 ccttacatca acccgtccct atagtgactc agagttatgc tgatcatcct cccgtgccta
     1681 caacaagcct gcaccaccag ccctatcggt gacccgacat ttgaaccaat atggagagta
     1741 aggagatcag agggtccaag cagggccgaa tctggcgaca gtagatataa atatgaaaat
     1801 tccccaacgc ttttactcat ggagctgcat ggctttctcg cggcaagcag ctggggtgaa
     1861 gccatttcca accttccctg caatgatgcg ccaccgatcg ttctcgtact cgtgcattgc
     1921 ccgctgcagc ctttgtacct attcccttca ttagcatgtg tgagacaggc gtagaaatac
     1981 atcaggagca aatcgaacca tttcgtcact ccaagctaca tccttagcct tcagcttggt
     2041 gcagtatcgg acttgcaagg taccgctcgt tcggcccggg aagaagtctg caatttgctt
     2101 ccacgtcaag ttcttgtttt ccttcaactc caccagtaga gcgtcctctt cgggagtaaa
     2161 cttcagtttt ggtcctcgag gcctgggggc tggatcaggc attccaggct ggcccaccac
     2221 actcggcggg cccgagggca aaggaggaga ttccacattc actccatgac gccctggggc
     2281 gcgcagcgct gcatggggcg ggagtcgatg gtgatggtga tgttgcggca gggccacaga
     2341 gtccgtgggc ccaaagccat ggtttgacat gggatgctgc tgagatgatg cgccatggac
     2401 accgaagagc atttcgggag agtgcgaggg ttcgtgaggg gcgtcagctg agaggactga
     2461 tagctgcggc cgcaggccat ggtcggggtt cccatcctct tcggttcatg aaccttgtgc
     2521 ccgtctccga tcggagaccg cgaaaaaacc ccgccctgtc aggggcgggg ttttttgcga
     2581 ctagtgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     2641 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2701 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2761 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2821 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2881 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2941 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3001 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3061 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3121 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3181 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3241 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3301 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3361 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3421 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3481 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagatctaca
     3541 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgagat tatcaaaaag
     3601 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3661 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3721 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3781 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagagccac gctcaccggc
     3841 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3901 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3961 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     4021 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     4081 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     4141 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     4201 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4261 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4321 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4381 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4441 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4501 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4561 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4621 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
     4681 ctgtagcggc accggtggtc tccccggagc gccactggac tctcaggctt tccattgggg
     4741 tactaacata gccatcaaat gccggcccgt gtttaatctc ctcgagatgg ctgggctcac
     4801 atgcgttcta ctcaaaaact cttataccta ggccctctcc caatcgtgta tgtttggacc
     4861 ttcctctctc acactttgga taacatcaag catactgcag attttgacct agttaacaag
     4921 gcacatcatg ctatcacttc caacactctt gctgagcgcg ttctgccttg cctttctggt
     4981 tctacgaagt atccagtccc gcctgcaacg ccgcagaaac gccaaaaggt ggggttgtca
     5041 accgccagct agagctccat cgggattctt tggcattccg ccgttcctcg gtggtcgcag
     5101 ataactccct aaattccctg agctaactct aagtcgacca tgccgtttat ggttagcgcc
     5161 tcccaaaaag gaatggccga cttagagtta cctcttgacc gactttttct ttcctccccc
     5221 ttacatttcg ttaccacaac acattcctat atccaaactc caggtacata actagtcgaa
     5281 atctctttta aatctagtca agaactagat acttaacctt catactgctt agagatagcc
     5341 tttgaagctc ttatttcgct ctttgtctca ctctcacctt cctcctcctc ctccaatcct
     5401 ttttgctcgg gactagtcca agactagtca acgattagta tgccgagcat tcgcgataac
     5461 gatctccgaa agtccccaga gtactgtcac tatctcgagg cagttaagga cggggagctt
     5521 acgctgccgg atttcaagat agtaagccga cccgactagc tttaagccta gttactgact
     5581 agtccacagg acgacaatgg cgtgcctgat atccatccat atgaagtcta ctgccgagtg
     5641 aagggatgcc tcaagcgtac agtgagtcta ctgctatact agtttctgac tagttattaa
     5701 ctagttgaca ggttccctct gccaacagaa acatattggt caagcacttg aaggacaaga
     5761 actcccacgg catggagttt acattgcaca atggacctcc cactatgaag gaactgatgg
     5821 aggccaaagg caagtcctat ctagattact tggtgactag tccctgacta gtcaagtagc
     5881 atggtatgaa ggcttgtttg aaggcactgt tctcccaacc ccgactccta ccaagaagcg
     5941 caagcgagct gcgtaagttt ctgtgagtct aactagtgta ttagctaata tatagcagca
     6001 ccaagtccaa ggaccacaat actaagggtg tcgagaattc gtgagtttct tctcccattt
     6061 caactagtcc ttgactagtc actaactact ttgcagcaac gagggtgaag ctggaaatga
     6121 tcaggacaat ggcgagggcc cgtaagtaca gccattcaat gcagactagt tgctaactaa
     6181 tctgtgacta gttcaagtgg tccgtacgcc gtgcataccc ctgtgactgg taggaatttg
     6241 agcaagcctg tcttgccgcg cgatgagaaa ggaaaggcaa gttacattca gccccgtacc
     6301 taggatcagt gctaatttat aacctctagc cactctttat gcagatccgc cgtgagggta
     6361 gcaaggcagc taaatcagct ggtgagaaag gaaccatacc ctgcaagacc tgtcgcaacg
     6421 caaagggcaa aggtaagcta tccaagctag tttgggacta gattctaact agtctcagca
     6481 ccgtgtggtt caaagccata ttgcgagttt tggcgctttt tctcatcgat tgacgaggca
     6541 aagggagcga gtatgcaacc tcgtaagtca gactcagaca aagaccaact agttattgac
     6601 cagtcatcta gaaggctctg ttgtggatct tgaggccctg gagagttcct ccaacaatcc
     6661 ggagacaagc aagtcctcgt cggactagtc actaactaga ctctaactag ttgcagacat
     6721 ggataatgca aaagagacaa gcaatgaaga aagtggtaag acatttctcc tttgtggttc
     6781 tggactagtc tttgactagt cacagtctta aacaaggaaa atgagcatga aaatgaggag
     6841 gaaaaggctg ctgagcccga ggaagtgcag ggtgatggca gacatggtag gttaatacct
     6901 tgttagttat tgctagtcac tgactagtca ataactagtc tctgaacacc ttgcaatcac
     6961 tccgtttgcg cagctgaaca gtggtgagga taatagtagt aagttattct agcttcagag
     7021 ttataggaga ctagatacta actagtatta gttgcaacta acctgg
//
