LOCUS       sequence-181736-        4288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of maize SCE1b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Augustine RC, York SL, Rytz TC, Vierstra RD
  TITLE     Defining the SUMO System in Maize: SUMOylation Is Up-Regulated 
            during Endosperm Development and Rapidly Induced by Stress.
  JOURNAL   Plant Physiol. 2016 Jul;171(3):2191-210. doi: 10.1104/pp.16.00353. 
            Epub 2016 May 15.
  PUBMED    27208252
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.00353"; journalName: "Plant Physiol"; date: 
            "2016-07"; volume: "171"; issue: "3"; pages: "2191-210"
COMMENT     Sequence Label: pRSF_ZmSCE1b-Myc
FEATURES             Location/Qualifiers
     source          1..4288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        183..201
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    202..226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             749..778
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             794..838
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(876..894)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      890..937
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1167..1982)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1167..1982)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1890..1909
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1983..2074)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2090..2839)
                     /direction=LEFT
                     /label=RSF ori
                     /note="Plasmids containing the RSF 1030 origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     complement(2100..2117)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2340..2359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3035..4117)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4079..4098
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4118..4195)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        4241..4259
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4260..4284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tagaaataat tttgtttaac tttaataagg agatatacca tgggcagcag ccatcaccat
       61 catcaccaca gccaggatcc gaattcgagc tcggcgcgcc tgcaggtcga caagcttgcg
      121 gccgcataat gcttaagtcg aacagaaagt aatcgtattg tacacggccg cataatcgaa
      181 attaatacga ctcactatag gggaattgtg agcggataac aattccccat cttagtatat
      241 tagttaagta taagaaggag atatacatat gtctggggga atcgcccgcg gccgccttgc
      301 cgaggagcgc aaggcctggc gcaagaacca cccgcacggt ttcgtcgcaa agccggagtc
      361 gctgcccgac gggacggtga acctgatgat ctggcagtgc accatccccg gcaagcaagg
      421 gactgactgg gaaggtggat atttccctct cacccttcat tttagtgagg attaccctag
      481 caagcctccc aagtgcaagt tccctcaggg tttcttccac ccaaatgtgt atccttctgg
      541 aacagtctgc ctttcgattc ttaatgagga cagtggttgg agaccagcta ttactgttaa
      601 gcagattctc gtcgggatcc aggacttgct agatcagcca aatcctgctg accctgctca
      661 aacagacgga tatcaccttt ttatccagga tcccacagaa tacaagaggc gcgttagact
      721 gcaggcgaag cagtatcctg cattggtcga acaaaaactc atctcagaag aggatctgtg
      781 actcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc
      841 tactagcgca gcttaattaa cctaggctgc tgccaccgct gagcaataac tagcataacc
      901 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaacctcagg catttgagaa
      961 gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc
     1021 ggctatttaa cgaccctgcc ctgaaccgac gacaagctga cgaccgggtc tccgcaagtg
     1081 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1141 atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa atgaaactgc
     1201 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     1261 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     1321 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     1381 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt
     1441 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     1501 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg gtcgctgtta
     1561 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     1621 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
     1681 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     1741 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     1801 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     1861 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     1921 gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc
     1981 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2041 tacatatttg aatgtattta gaaaaataaa caaataggca tgcagcgctc ttccgcttcc
     2101 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggtgtc agctcactca
     2161 aaagcggtaa tacggttatc cacagaatca ggggataaag ccggaaagaa catgtgagca
     2221 aaaagcaaag caccggaaga agccaacgcc gcaggcgttt ttccataggc tccgcccccc
     2281 tgacgagcat cacaaaaatc gacgctcaag ccagaggtgg cgaaacccga caggactata
     2341 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2401 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2461 acgctgttgg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2521 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2581 ggtaagacac gacttatcgc cactggcagc agccattggt aactgattta gaggactttg
     2641 tcttgaagtt atgcacctgt taaggctaaa ctgaaagaac agattttggt gagtgcggtc
     2701 ctccaaccca cttaccttgg ttcaaagagt tggtagctca gcgaaccttg agaaaaccac
     2761 cgttggtagc ggtggttttt ctttatttat gagatgatga atcaatcggt ctatcaagtc
     2821 aacgaacagc tattccgtta ctctagattt cagtgcaatt tatctcttca aatgtagcac
     2881 ctgaagtcag ccccatacga tataagttgt aattctcatg ttagtcatgc cccgcgccca
     2941 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3001 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3061 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3121 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3181 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3241 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3301 atcccactac cgagatgtcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3361 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3421 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3481 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     3541 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     3601 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     3661 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     3721 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     3781 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     3841 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     3901 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     3961 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4021 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4081 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4141 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4201 tctcgacgct ctcccttatg cgactcctgc attaggaaat taatacgact cactataggg
     4261 gaattgtgag cggataacaa ttcccctg
//
