LOCUS       sequence-181739-        4239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 promoter coxA gene of Aspergillus niger between FS1 and FS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB1_P_12_asn_coxA
FEATURES             Location/Qualifiers
     source          1..4239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(140..159)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             151..966
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             151..966
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(224..243)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1051..1639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1540..1559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1793..1810
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1927..1948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1963..1968,1969..1986,1987..1993)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2001..2017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2006..2028
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2025..2041
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2025..2041
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2060..2079
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2060..2078
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3547..3565)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3548..3565)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3579..3596)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3579..3595)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3588..3610)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3736..4191)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3873..3894)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4085..4104
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gatctctgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
       61 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaacaat aaaactgtct
      121 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc
      181 tctaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
      241 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
      301 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
      361 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
      421 cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta
      481 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
      541 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
      601 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
      661 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
      721 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
      781 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
      841 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
      901 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
      961 ttctaagaat taattcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1021 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1081 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1141 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1201 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1261 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1321 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1381 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1441 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1501 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1561 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1621 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1681 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     1741 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     1801 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     1861 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     1921 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     1981 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2041 ccatgattac gccaagctct aatacgactc actataggga aagctcggta ccacgcatgc
     2101 tgcagacgcg ttacgtatcg gatccagaat tcgtgatgaa gacgccatgt gtcctggtgg
     2161 gtgggttgca atgacggcag acaaggcaag gacgtttgga gatgagggag tttggaggtt
     2221 gaggtgagtt tgaagccgct tcggcccgtg gtcgcctgcc cttagtcagc gccggggatt
     2281 gattggttgc ggagcttctc cggctgcccg cagtgacctg gtccgcagac ctactcgatt
     2341 gggtgtccgg cttagggctt agggtctata atctgctcca gtggtcatct gcggtctgcc
     2401 gtagataagg aacacatgcc ccggccgagc aatggccgcg ttcagcccgg gatcagccgg
     2461 tgctaaactg cgagagcgga cgctagtgtc ggttcattgc gaacgaacaa atgccggcgc
     2521 catatgttcc cccgggctaa aatgaattat catacatcac ttaatacctt acatcaaccc
     2581 gtccctatag tgactcagag ttatgctgat catcctcccg tgcctacaac aagcctgcac
     2641 caccagccct atcggtgacc cgacatttga accaatatgg agagtaagga gatcagaggg
     2701 tccaagcagg gccgaatctg gcgacagtag atataaatat gaaaattccc caacgctttt
     2761 actcatggag ctgcatggct ttctcgcggc aagcagctgg ggtgaagcca tttccaacct
     2821 tccctgcaat gatgcgccac cgatcgttct cgtactcgtg cattgcccgc tgcagccttt
     2881 gtacctattc ccttcattag catgtgtgag acaggcgtag aaatacatca ggagcaaatc
     2941 gaaccatttc gtcactccaa gctacatcct tagccttcag cttggtgcag tatcggactt
     3001 gcaaggtacc gctcgttcgg cccgggaaga agtctgcaat ttgcttccac gtcaagttct
     3061 tgttttcctt caactccacc agtagagcgt cctcttcggg agtaaacttc agttttggtc
     3121 ctcgaggcct gggggctgga tcaggcattc caggctggcc caccacactc ggcgggcccg
     3181 agggcaaagg aggagattcc acattcactc catgacgccc tggggcgcgc agcgctgcat
     3241 ggggcgggag tcgatggtga tggtgatgtt gcggcagggc cacagagtcc gtgggcccaa
     3301 agccatggtt tgacatggga tgctgctgag atgatgcgcc atggacaccg aagagcattt
     3361 cgggagagtg cgagggttcg tgaggggcgt cagctgagag gactgatagc tgcggccgca
     3421 ggccatggtc ggggttccca tcctcttcgg ttctccgcgt cttcatctga attcgtcgac
     3481 aagcttctcg agcctaggct agctctagac cacacgtgtg ggggcccgag ctcgcggccg
     3541 ctgtattcta tagtgtcacc taaatggccg cacaattcac tggccgtcgt tttacaacgt
     3601 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3661 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3721 ctgaatggcg aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     3781 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3841 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3901 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3961 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     4021 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     4081 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     4141 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     4201 cttttcgggg aaatgtgcgc ggaaccccta tttgtttaa
//
