LOCUS       sequence-181766-        8411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A0502
FEATURES             Location/Qualifiers
     source          1..8411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             2788..2844
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2845..6948
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2845..6948
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6961..6981
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6994..7242
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        7319..7423
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgccgt cgagctgcag gtcaacggat
      901 caggatattc ttgtttaaga tgttgaactc tatggaggtt tgtatgaact gatgatctag
      961 gaccggataa gttcccttct tcatagcgaa cttattcaaa gaatgttttg tgtatcattc
     1021 ttgttacatt gttattaatg aaaaaatatt attggtcatt ggactgaaca cgagtgttaa
     1081 atatggacca ggccccaaat aagatccatt gatatatgaa ttaaataaca agaataaatc
     1141 gagtcaccaa accacttgcc ttttttaacg agacttgttc accaacttga tacaaaagtc
     1201 attatcctat gcaaatcaat aatcatacaa aaatatccaa taacactaaa aaattaaaag
     1261 aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact
     1321 gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat
     1381 aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc
     1441 aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa
     1501 aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttagaaa
     1561 ttgtggttgt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg
     1621 agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta
     1681 gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta
     1741 ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt
     1801 ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa
     1861 tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta
     1921 ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt
     1981 ttagattctg ttaatcttag atcgaacacg attttctggg tttgatcgtt agatatcatc
     2041 ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg
     2101 tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg
     2161 tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acaggcctgc aggaaaaatg
     2221 gatcattatc ttgatattag acttagacct gatccagaat ttccaccagc tcaacttatg
     2281 tctgttcttt ttggaaaact tcatcaagct cttgttgctc aaggaggaga tagaattgga
     2341 gtttcttttc ctgatcttga tgaatcaaga tcaagacttg gagaaagact tagaattcat
     2401 gcttctgctg atgatcttag agctttgctt gctagacctt ggcttgaagg acttagagat
     2461 catcttcaat ttggagaacc agctgttgtt ccacatccaa ctccttatag acaagtttca
     2521 agagttcaag ctaaatctaa tccagaaaga cttagaagaa gacttatgag aagacatgat
     2581 ctttctgaag aagaagctag aaaaagaatt cctgatactg ttgctagagc tttggatttg
     2641 ccttttgtta cacttagatc acaatctact ggacaacatt ttagactttt tattagacat
     2701 ggaccacttc aagttactgc tgaagaagga ggatttactt gttatggact ttctaaggga
     2761 ggttttgttc cttggtttgg atctggagct actaattttt ctcttcttaa gcaagctgga
     2821 gatgttgaag aaaatcctgg acccatggat aagaagtact ctatcggact cgatatcgga
     2881 actaactctg tgggatgggc tgtgatcacc gatgagtaca aggtgccatc taagaagttc
     2941 aaggttctcg gaaacaccga taggcactct atcaagaaaa accttatcgg tgctctcctc
     3001 ttcgattctg gtgaaactgc tgaggctacc agactcaaga gaaccgctag aagaaggtac
     3061 accagaagaa agaacaggat ctgctacctc caagagatct tctctaacga gatggctaaa
     3121 gtggatgatt cattcttcca caggctcgaa gagtcattcc tcgtggaaga agataagaag
     3181 cacgagaggc accctatctt cggaaacatc gttgatgagg tggcatacca cgagaagtac
     3241 cctactatct accacctcag aaagaagctc gttgattcta ctgataaggc tgatctcagg
     3301 ctcatctacc tcgctctcgc tcacatgatc aagttcagag gacacttcct catcgagggt
     3361 gatctcaacc ctgataactc tgatgtggat aagttgttca tccagctcgt gcagacctac
     3421 aaccagcttt tcgaagagaa ccctatcaac gcttcaggtg tggatgctaa ggctatcctc
     3481 tctgctaggc tctctaagtc aagaaggctt gagaacctca ttgctcagct ccctggtgag
     3541 aagaagaacg gacttttcgg aaacttgatc gctctctctc tcggactcac ccctaacttc
     3601 aagtctaact tcgatctcgc tgaggatgca aagctccagc tctcaaagga tacctacgat
     3661 gatgatctcg ataacctcct cgctcagatc ggagatcagt acgctgattt gttcctcgct
     3721 gctaagaacc tctctgatgc tatcctcctc agtgatatcc tcagagtgaa caccgagatc
     3781 accaaggctc cactctcagc ttctatgatc aagagatacg atgagcacca ccaggatctc
     3841 acacttctca aggctcttgt tagacagcag ctcccagaga agtacaaaga gattttcttc
     3901 gatcagtcta agaacggata cgctggttac atcgatggtg gtgcatctca agaagagttc
     3961 tacaagttca tcaagcctat cctcgagaag atggatggaa ccgaggaact cctcgtgaag
     4021 ctcaatagag aggatcttct cagaaagcag aggaccttcg ataacggatc tatccctcat
     4081 cagatccacc tcggagagtt gcacgctatc cttagaaggc aagaggattt ctacccattc
     4141 ctcaaggata acagggaaaa gattgagaag attctcacct tcagaatccc ttactacgtg
     4201 ggacctctcg ctagaggaaa ctcaagattc gcttggatga ccagaaagtc tgaggaaacc
     4261 atcacccctt ggaacttcga agaggtggtg gataagggtg ctagtgctca gtctttcatc
     4321 gagaggatga ccaacttcga taagaacctt ccaaacgaga aggtgctccc taagcactct
     4381 ttgctctacg agtacttcac cgtgtacaac gagttgacca aggttaagta cgtgaccgag
     4441 ggaatgagga agcctgcttt tttgtcaggt gagcaaaaga aggctatcgt tgatctcttg
     4501 ttcaagacca acagaaaggt gaccgtgaag cagctcaaag aggattactt caagaaaatc
     4561 gagtgcttcg attcagttga gatttctggt gttgaggata ggttcaacgc atctctcgga
     4621 acctaccacg atctcctcaa gatcattaag gataaggatt tcttggataa cgaggaaaac
     4681 gaggatatct tggaggatat cgttcttacc ctcaccctct ttgaagatag agagatgatt
     4741 gaagaaaggc tcaagaccta cgctcatctc ttcgatgata aggtgatgaa gcagttgaag
     4801 agaagaagat acactggttg gggaaggctc tcaagaaagc tcattaacgg aatcagggat
     4861 aagcagtctg gaaagacaat ccttgatttc ctcaagtctg atggattcgc taacagaaac
     4921 ttcatgcagc tcatccacga tgattctctc acctttaaag aggatatcca gaaggctcag
     4981 gtttcaggac agggtgatag tctccatgag catatcgcta acctcgctgg atctcctgca
     5041 atcaagaagg gaatcctcca gactgtgaag gttgtggatg agttggtgaa ggtgatggga
     5101 aggcataagc ctgagaacat cgtgatcgaa atggctagag agaaccagac cactcagaag
     5161 ggacagaaga actctaggga aaggatgaag aggatcgagg aaggtatcaa agagcttgga
     5221 tctcagatcc tcaaagagca ccctgttgag aacactcagc tccagaatga gaagctctac
     5281 ctctactacc tccagaacgg aagggatatg tatgtggatc aagagttgga tatcaacagg
     5341 ctctctgatt acgatgttga tcatatcgtg ccacagtcat tcttgaagga tgattctatc
     5401 gataacaagg tgctcaccag gtctgataag aacaggggta agagtgataa cgtgccaagt
     5461 gaagaggttg tgaagaaaat gaagaactat tggaggcagc tcctcaacgc taagctcatc
     5521 actcagagaa agttcgataa cttgactaag gctgagaggg gaggactctc tgaattggat
     5581 aaggcaggat tcatcaagag gcagcttgtg gaaaccaggc agatcactaa gcacgttgca
     5641 cagatcctcg attctaggat gaacaccaag tacgatgaga acgataagtt gatcagggaa
     5701 gtgaaggtta tcaccctcaa gtcaaagctc gtgtctgatt tcagaaagga tttccaattc
     5761 tacaaggtga gggaaatcaa caactaccac cacgctcacg atgcttacct taacgctgtt
     5821 gttggaaccg ctctcatcaa gaagtatcct aagctcgagt cagagttcgt gtacggtgat
     5881 tacaaggtgt acgatgtgag gaagatgatc gctaagtctg agcaagagat cggaaaggct
     5941 accgctaagt atttcttcta ctctaacatc atgaatttct tcaagaccga gattaccctc
     6001 gctaacggtg agatcagaaa gaggccactc atcgagacaa acggtgaaac aggtgagatc
     6061 gtgtgggata agggaaggga tttcgctacc gttagaaagg tgctctctat gccacaggtg
     6121 aacatcgtta agaaaaccga ggtgcagacc ggtggattct ctaaagagtc tatcctccct
     6181 aagaggaact ctgataagct cattgctagg aagaaggatt gggaccctaa gaaatacggt
     6241 ggtttcgatt ctcctaccgt ggcttactct gttctcgttg tggctaaggt tgagaaggga
     6301 aagagtaaga agctcaagtc tgttaaggaa cttctcggaa tcactatcat ggaaaggtca
     6361 tctttcgaga agaacccaat cgatttcctc gaggctaagg gatacaaaga ggttaagaag
     6421 gatctcatca tcaagctccc aaagtactca ctcttcgaac tcgagaacgg tagaaagagg
     6481 atgctcgctt ctgctggtga gcttcaaaag ggaaacgagc ttgctctccc atctaagtac
     6541 gttaactttc tttacctcgc ttctcactac gagaagttga agggatctcc agaagataac
     6601 gagcagaagc aacttttcgt tgagcagcac aagcactact tggatgagat catcgagcag
     6661 atctctgagt tctctaaaag ggtgatcctc gctgatgcaa acctcgataa ggtgttgtct
     6721 gcttacaaca agcacagaga taagcctatc agggaacagg cagagaacat catccatctc
     6781 ttcaccctta ccaacctcgg tgctcctgct gctttcaagt acttcgatac aaccatcgat
     6841 aggaagagat acacctctac caaagaagtg ctcgatgcta ccctcatcca tcagtctatc
     6901 actggactct acgagactag gatcgatctc tcacagctcg gtggtgattc aagggctgat
     6961 cctaagaaga agaggaaggt ttgacgtcga cgatatgaag atgaagatga aatatttggt
     7021 gtgtcaaata aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat
     7081 gaatttgtgg ctttttctaa tattaaatga atgtaagatc acattataat gaataaacaa
     7141 atgtttctat aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg
     7201 tatgtgctat ggtatggact atggaatatg attaaagata agccagagct ctggtgacgg
     7261 acgtgggcag gtgtgatgaa aagccttagg gtcaggtggc acttttcggg gaaatgtgcg
     7321 atgaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc taatgagaca
     7381 ataaccctga taaatgcttc aattatattg aaaaaggaag agtatgagta tccaacattt
     7441 ccgtgtcgcc cttattccct tttttgcggc cttctgcctt cctgtttttg ctcacccaga
     7501 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcccgagtgg gttacattga
     7561 actggatctc aacagcggca aaatcttaga gagttttcgc cccgaagaac gcttcccaat
     7621 gatgagcact ttcaaagttc tgctatgtgg cgcggtgtta tcccgtattg atgctgggca
     7681 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt attcaccagt
     7741 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgtagtg ctgccataac
     7801 catgagtgat aacactgcgg ccaacttact tctgacaacg attggaggac cgaaggagct
     7861 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7921 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7981 aactctccgc aaactattaa ctggcgaact acttactctt gcttcacgcc agcaactcat
     8041 tgactggatg gaggcggaca aagttgcagg accacttctg cgctcggcac ttccggctgg
     8101 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8161 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8221 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8281 gtaactgtca gacgaaggga aataaatagt agcccgcctg atgtgcgggc ttttttttgg
     8341 tggtggttac cagtgtcgct tagctaccgt aggtcctctt aggcggtccg attagcgaca
     8401 tagtcatcaa t
//
