LOCUS       sequence-181777-        8073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: 35S, Gene: TREX2-P2A-AtCas9, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 8073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A0901
FEATURES             Location/Qualifiers
     source          1..8073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1366..1711
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1654..1674
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2450..2506
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2507..6610
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2507..6610
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6623..6643
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6656..6904
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        6981..7085
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgccag atttgccttt tcaatttcag
      901 aaagaatgct aacccacaga tggttagaga ggcttacgca gcaggtatca tcaagacgat
      961 ctacccgagc aataatctcc aggaaatcaa ataccttccc aagaaggtta aagatgcagt
     1021 caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag
     1081 aagtactatt ccagtatgga cgattcaagg cttgcttcac aaaccaaggc aagtaataga
     1141 gattggagtc tctaaaaagg tagttcccac tgaatcaaag gccatggagt caaagattca
     1201 aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt
     1261 acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta
     1321 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca
     1381 aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt
     1441 gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
     1501 catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
     1561 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
     1621 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
     1681 ataaggaagt tcatttcatt tggagagaac acgggggact cctgcaggaa aaatgtctga
     1741 gccacctcgg gctgagacct ttgtattcct ggacctagaa gccactgggc tcccaaacat
     1801 ggaccctgag attgcagaga tatccctttt tgctgttcac cgctcttccc tggagaaccc
     1861 agaacgggat gattctggtt ccttggtgct gccccgtgtt ctggacaagc tcacactgtg
     1921 catgtgcccg gagcgcccct ttactgccaa ggccagtgag attactggtt tgagcagcga
     1981 aagcctgatg cactgcggga aggctggttt caatggcgct gtggtaagga cactgcaggg
     2041 cttcctaagc cgccaggagg gccccatctg ccttgtggcc cacaatggct tcgattatga
     2101 cttcccactg ctgtgcacgg agctacaacg tctgggtgcc catctgcccc aagacactgt
     2161 ctgcctggac acactgcctg cattgcgggg cctggaccgt gctcacagcc acggcaccag
     2221 ggctcaaggc cgcaaaagct acagcctggc cagtctcttc caccgctact tccaggctga
     2281 acccagtgct gcccattcag cagaaggtga tgtgcacacc ctgcttctga tcttcctgca
     2341 tcgtgctcct gagctgctcg cctgggcaga tgagcaggcc cgcagctggg ctcatattga
     2401 gcccatgtac gtgccacctg atggtccaag cctcgaagcc ggatctggag ctactaattt
     2461 ttctcttctt aagcaagctg gagatgttga agaaaatcct ggacccatgg ataagaagta
     2521 ctctatcgga ctcgatatcg gaactaactc tgtgggatgg gctgtgatca ccgatgagta
     2581 caaggtgcca tctaagaagt tcaaggttct cggaaacacc gataggcact ctatcaagaa
     2641 aaaccttatc ggtgctctcc tcttcgattc tggtgaaact gctgaggcta ccagactcaa
     2701 gagaaccgct agaagaaggt acaccagaag aaagaacagg atctgctacc tccaagagat
     2761 cttctctaac gagatggcta aagtggatga ttcattcttc cacaggctcg aagagtcatt
     2821 cctcgtggaa gaagataaga agcacgagag gcaccctatc ttcggaaaca tcgttgatga
     2881 ggtggcatac cacgagaagt accctactat ctaccacctc agaaagaagc tcgttgattc
     2941 tactgataag gctgatctca ggctcatcta cctcgctctc gctcacatga tcaagttcag
     3001 aggacacttc ctcatcgagg gtgatctcaa ccctgataac tctgatgtgg ataagttgtt
     3061 catccagctc gtgcagacct acaaccagct tttcgaagag aaccctatca acgcttcagg
     3121 tgtggatgct aaggctatcc tctctgctag gctctctaag tcaagaaggc ttgagaacct
     3181 cattgctcag ctccctggtg agaagaagaa cggacttttc ggaaacttga tcgctctctc
     3241 tctcggactc acccctaact tcaagtctaa cttcgatctc gctgaggatg caaagctcca
     3301 gctctcaaag gatacctacg atgatgatct cgataacctc ctcgctcaga tcggagatca
     3361 gtacgctgat ttgttcctcg ctgctaagaa cctctctgat gctatcctcc tcagtgatat
     3421 cctcagagtg aacaccgaga tcaccaaggc tccactctca gcttctatga tcaagagata
     3481 cgatgagcac caccaggatc tcacacttct caaggctctt gttagacagc agctcccaga
     3541 gaagtacaaa gagattttct tcgatcagtc taagaacgga tacgctggtt acatcgatgg
     3601 tggtgcatct caagaagagt tctacaagtt catcaagcct atcctcgaga agatggatgg
     3661 aaccgaggaa ctcctcgtga agctcaatag agaggatctt ctcagaaagc agaggacctt
     3721 cgataacgga tctatccctc atcagatcca cctcggagag ttgcacgcta tccttagaag
     3781 gcaagaggat ttctacccat tcctcaagga taacagggaa aagattgaga agattctcac
     3841 cttcagaatc ccttactacg tgggacctct cgctagagga aactcaagat tcgcttggat
     3901 gaccagaaag tctgaggaaa ccatcacccc ttggaacttc gaagaggtgg tggataaggg
     3961 tgctagtgct cagtctttca tcgagaggat gaccaacttc gataagaacc ttccaaacga
     4021 gaaggtgctc cctaagcact ctttgctcta cgagtacttc accgtgtaca acgagttgac
     4081 caaggttaag tacgtgaccg agggaatgag gaagcctgct tttttgtcag gtgagcaaaa
     4141 gaaggctatc gttgatctct tgttcaagac caacagaaag gtgaccgtga agcagctcaa
     4201 agaggattac ttcaagaaaa tcgagtgctt cgattcagtt gagatttctg gtgttgagga
     4261 taggttcaac gcatctctcg gaacctacca cgatctcctc aagatcatta aggataagga
     4321 tttcttggat aacgaggaaa acgaggatat cttggaggat atcgttctta ccctcaccct
     4381 ctttgaagat agagagatga ttgaagaaag gctcaagacc tacgctcatc tcttcgatga
     4441 taaggtgatg aagcagttga agagaagaag atacactggt tggggaaggc tctcaagaaa
     4501 gctcattaac ggaatcaggg ataagcagtc tggaaagaca atccttgatt tcctcaagtc
     4561 tgatggattc gctaacagaa acttcatgca gctcatccac gatgattctc tcacctttaa
     4621 agaggatatc cagaaggctc aggtttcagg acagggtgat agtctccatg agcatatcgc
     4681 taacctcgct ggatctcctg caatcaagaa gggaatcctc cagactgtga aggttgtgga
     4741 tgagttggtg aaggtgatgg gaaggcataa gcctgagaac atcgtgatcg aaatggctag
     4801 agagaaccag accactcaga agggacagaa gaactctagg gaaaggatga agaggatcga
     4861 ggaaggtatc aaagagcttg gatctcagat cctcaaagag caccctgttg agaacactca
     4921 gctccagaat gagaagctct acctctacta cctccagaac ggaagggata tgtatgtgga
     4981 tcaagagttg gatatcaaca ggctctctga ttacgatgtt gatcatatcg tgccacagtc
     5041 attcttgaag gatgattcta tcgataacaa ggtgctcacc aggtctgata agaacagggg
     5101 taagagtgat aacgtgccaa gtgaagaggt tgtgaagaaa atgaagaact attggaggca
     5161 gctcctcaac gctaagctca tcactcagag aaagttcgat aacttgacta aggctgagag
     5221 gggaggactc tctgaattgg ataaggcagg attcatcaag aggcagcttg tggaaaccag
     5281 gcagatcact aagcacgttg cacagatcct cgattctagg atgaacacca agtacgatga
     5341 gaacgataag ttgatcaggg aagtgaaggt tatcaccctc aagtcaaagc tcgtgtctga
     5401 tttcagaaag gatttccaat tctacaaggt gagggaaatc aacaactacc accacgctca
     5461 cgatgcttac cttaacgctg ttgttggaac cgctctcatc aagaagtatc ctaagctcga
     5521 gtcagagttc gtgtacggtg attacaaggt gtacgatgtg aggaagatga tcgctaagtc
     5581 tgagcaagag atcggaaagg ctaccgctaa gtatttcttc tactctaaca tcatgaattt
     5641 cttcaagacc gagattaccc tcgctaacgg tgagatcaga aagaggccac tcatcgagac
     5701 aaacggtgaa acaggtgaga tcgtgtggga taagggaagg gatttcgcta ccgttagaaa
     5761 ggtgctctct atgccacagg tgaacatcgt taagaaaacc gaggtgcaga ccggtggatt
     5821 ctctaaagag tctatcctcc ctaagaggaa ctctgataag ctcattgcta ggaagaagga
     5881 ttgggaccct aagaaatacg gtggtttcga ttctcctacc gtggcttact ctgttctcgt
     5941 tgtggctaag gttgagaagg gaaagagtaa gaagctcaag tctgttaagg aacttctcgg
     6001 aatcactatc atggaaaggt catctttcga gaagaaccca atcgatttcc tcgaggctaa
     6061 gggatacaaa gaggttaaga aggatctcat catcaagctc ccaaagtact cactcttcga
     6121 actcgagaac ggtagaaaga ggatgctcgc ttctgctggt gagcttcaaa agggaaacga
     6181 gcttgctctc ccatctaagt acgttaactt tctttacctc gcttctcact acgagaagtt
     6241 gaagggatct ccagaagata acgagcagaa gcaacttttc gttgagcagc acaagcacta
     6301 cttggatgag atcatcgagc agatctctga gttctctaaa agggtgatcc tcgctgatgc
     6361 aaacctcgat aaggtgttgt ctgcttacaa caagcacaga gataagccta tcagggaaca
     6421 ggcagagaac atcatccatc tcttcaccct taccaacctc ggtgctcctg ctgctttcaa
     6481 gtacttcgat acaaccatcg ataggaagag atacacctct accaaagaag tgctcgatgc
     6541 taccctcatc catcagtcta tcactggact ctacgagact aggatcgatc tctcacagct
     6601 cggtggtgat tcaagggctg atcctaagaa gaagaggaag gtttgacgtc gacgatatga
     6661 agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt
     6721 ttttcttggc ttgttgtgtt atgaatttgt ggctttttct aatattaaat gaatgtaaga
     6781 tcacattata atgaataaac aaatgtttct ataatccatt gtgaatgttt tgttggatct
     6841 cttctgcagc atataactac tgtatgtgct atggtatgga ctatggaata tgattaaaga
     6901 taagccagag ctctggtgac ggacgtgggc aggtgtgatg aaaagcctta gggtcaggtg
     6961 gcacttttcg gggaaatgtg cgatgaaccc ctatttgttt atttttctaa atacattcaa
     7021 atatgtatcc gctaatgaga caataaccct gataaatgct tcaattatat tgaaaaagga
     7081 agagtatgag tatccaacat ttccgtgtcg cccttattcc cttttttgcg gccttctgcc
     7141 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7201 gtgcccgagt gggttacatt gaactggatc tcaacagcgg caaaatctta gagagttttc
     7261 gccccgaaga acgcttccca atgatgagca ctttcaaagt tctgctatgt ggcgcggtgt
     7321 tatcccgtat tgatgctggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7381 acttggttga gtattcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7441 aattatgtag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7501 cgattggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7561 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7621 cgatgcctgt agcaatggca acaactctcc gcaaactatt aactggcgaa ctacttactc
     7681 ttgcttcacg ccagcaactc attgactgga tggaggcgga caaagttgca ggaccacttc
     7741 tgcgctcggc acttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7801 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7861 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7921 gtgcctcact gattaagcat tggtaactgt cagacgaagg gaaataaata gtagcccgcc
     7981 tgatgtgcgg gctttttttt ggtggtggtt accagtgtcg cttagctacc gtaggtcctc
     8041 ttaggcggtc cgattagcga catagtcatc aat
//
