LOCUS       sequence-181778-        8558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: AtUbi10, Gene: TREX2-P2A-AtCas9, Terminator: 
            HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 8558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A0902
FEATURES             Location/Qualifiers
     source          1..8558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             2935..2991
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2992..7095
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2992..7095
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7108..7128
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7141..7389
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        7466..7570
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgccgt cgagctgcag gtcaacggat
      901 caggatattc ttgtttaaga tgttgaactc tatggaggtt tgtatgaact gatgatctag
      961 gaccggataa gttcccttct tcatagcgaa cttattcaaa gaatgttttg tgtatcattc
     1021 ttgttacatt gttattaatg aaaaaatatt attggtcatt ggactgaaca cgagtgttaa
     1081 atatggacca ggccccaaat aagatccatt gatatatgaa ttaaataaca agaataaatc
     1141 gagtcaccaa accacttgcc ttttttaacg agacttgttc accaacttga tacaaaagtc
     1201 attatcctat gcaaatcaat aatcatacaa aaatatccaa taacactaaa aaattaaaag
     1261 aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact
     1321 gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat
     1381 aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc
     1441 aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa
     1501 aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttagaaa
     1561 ttgtggttgt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg
     1621 agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta
     1681 gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta
     1741 ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt
     1801 ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa
     1861 tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta
     1921 ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt
     1981 ttagattctg ttaatcttag atcgaacacg attttctggg tttgatcgtt agatatcatc
     2041 ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg
     2101 tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg
     2161 tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acaggcctgc aggaaaaatg
     2221 tctgagccac ctcgggctga gacctttgta ttcctggacc tagaagccac tgggctccca
     2281 aacatggacc ctgagattgc agagatatcc ctttttgctg ttcaccgctc ttccctggag
     2341 aacccagaac gggatgattc tggttccttg gtgctgcccc gtgttctgga caagctcaca
     2401 ctgtgcatgt gcccggagcg cccctttact gccaaggcca gtgagattac tggtttgagc
     2461 agcgaaagcc tgatgcactg cgggaaggct ggtttcaatg gcgctgtggt aaggacactg
     2521 cagggcttcc taagccgcca ggagggcccc atctgccttg tggcccacaa tggcttcgat
     2581 tatgacttcc cactgctgtg cacggagcta caacgtctgg gtgcccatct gccccaagac
     2641 actgtctgcc tggacacact gcctgcattg cggggcctgg accgtgctca cagccacggc
     2701 accagggctc aaggccgcaa aagctacagc ctggccagtc tcttccaccg ctacttccag
     2761 gctgaaccca gtgctgccca ttcagcagaa ggtgatgtgc acaccctgct tctgatcttc
     2821 ctgcatcgtg ctcctgagct gctcgcctgg gcagatgagc aggcccgcag ctgggctcat
     2881 attgagccca tgtacgtgcc acctgatggt ccaagcctcg aagccggatc tggagctact
     2941 aatttttctc ttcttaagca agctggagat gttgaagaaa atcctggacc catggataag
     3001 aagtactcta tcggactcga tatcggaact aactctgtgg gatgggctgt gatcaccgat
     3061 gagtacaagg tgccatctaa gaagttcaag gttctcggaa acaccgatag gcactctatc
     3121 aagaaaaacc ttatcggtgc tctcctcttc gattctggtg aaactgctga ggctaccaga
     3181 ctcaagagaa ccgctagaag aaggtacacc agaagaaaga acaggatctg ctacctccaa
     3241 gagatcttct ctaacgagat ggctaaagtg gatgattcat tcttccacag gctcgaagag
     3301 tcattcctcg tggaagaaga taagaagcac gagaggcacc ctatcttcgg aaacatcgtt
     3361 gatgaggtgg cataccacga gaagtaccct actatctacc acctcagaaa gaagctcgtt
     3421 gattctactg ataaggctga tctcaggctc atctacctcg ctctcgctca catgatcaag
     3481 ttcagaggac acttcctcat cgagggtgat ctcaaccctg ataactctga tgtggataag
     3541 ttgttcatcc agctcgtgca gacctacaac cagcttttcg aagagaaccc tatcaacgct
     3601 tcaggtgtgg atgctaaggc tatcctctct gctaggctct ctaagtcaag aaggcttgag
     3661 aacctcattg ctcagctccc tggtgagaag aagaacggac ttttcggaaa cttgatcgct
     3721 ctctctctcg gactcacccc taacttcaag tctaacttcg atctcgctga ggatgcaaag
     3781 ctccagctct caaaggatac ctacgatgat gatctcgata acctcctcgc tcagatcgga
     3841 gatcagtacg ctgatttgtt cctcgctgct aagaacctct ctgatgctat cctcctcagt
     3901 gatatcctca gagtgaacac cgagatcacc aaggctccac tctcagcttc tatgatcaag
     3961 agatacgatg agcaccacca ggatctcaca cttctcaagg ctcttgttag acagcagctc
     4021 ccagagaagt acaaagagat tttcttcgat cagtctaaga acggatacgc tggttacatc
     4081 gatggtggtg catctcaaga agagttctac aagttcatca agcctatcct cgagaagatg
     4141 gatggaaccg aggaactcct cgtgaagctc aatagagagg atcttctcag aaagcagagg
     4201 accttcgata acggatctat ccctcatcag atccacctcg gagagttgca cgctatcctt
     4261 agaaggcaag aggatttcta cccattcctc aaggataaca gggaaaagat tgagaagatt
     4321 ctcaccttca gaatccctta ctacgtggga cctctcgcta gaggaaactc aagattcgct
     4381 tggatgacca gaaagtctga ggaaaccatc accccttgga acttcgaaga ggtggtggat
     4441 aagggtgcta gtgctcagtc tttcatcgag aggatgacca acttcgataa gaaccttcca
     4501 aacgagaagg tgctccctaa gcactctttg ctctacgagt acttcaccgt gtacaacgag
     4561 ttgaccaagg ttaagtacgt gaccgaggga atgaggaagc ctgctttttt gtcaggtgag
     4621 caaaagaagg ctatcgttga tctcttgttc aagaccaaca gaaaggtgac cgtgaagcag
     4681 ctcaaagagg attacttcaa gaaaatcgag tgcttcgatt cagttgagat ttctggtgtt
     4741 gaggataggt tcaacgcatc tctcggaacc taccacgatc tcctcaagat cattaaggat
     4801 aaggatttct tggataacga ggaaaacgag gatatcttgg aggatatcgt tcttaccctc
     4861 accctctttg aagatagaga gatgattgaa gaaaggctca agacctacgc tcatctcttc
     4921 gatgataagg tgatgaagca gttgaagaga agaagataca ctggttgggg aaggctctca
     4981 agaaagctca ttaacggaat cagggataag cagtctggaa agacaatcct tgatttcctc
     5041 aagtctgatg gattcgctaa cagaaacttc atgcagctca tccacgatga ttctctcacc
     5101 tttaaagagg atatccagaa ggctcaggtt tcaggacagg gtgatagtct ccatgagcat
     5161 atcgctaacc tcgctggatc tcctgcaatc aagaagggaa tcctccagac tgtgaaggtt
     5221 gtggatgagt tggtgaaggt gatgggaagg cataagcctg agaacatcgt gatcgaaatg
     5281 gctagagaga accagaccac tcagaaggga cagaagaact ctagggaaag gatgaagagg
     5341 atcgaggaag gtatcaaaga gcttggatct cagatcctca aagagcaccc tgttgagaac
     5401 actcagctcc agaatgagaa gctctacctc tactacctcc agaacggaag ggatatgtat
     5461 gtggatcaag agttggatat caacaggctc tctgattacg atgttgatca tatcgtgcca
     5521 cagtcattct tgaaggatga ttctatcgat aacaaggtgc tcaccaggtc tgataagaac
     5581 aggggtaaga gtgataacgt gccaagtgaa gaggttgtga agaaaatgaa gaactattgg
     5641 aggcagctcc tcaacgctaa gctcatcact cagagaaagt tcgataactt gactaaggct
     5701 gagaggggag gactctctga attggataag gcaggattca tcaagaggca gcttgtggaa
     5761 accaggcaga tcactaagca cgttgcacag atcctcgatt ctaggatgaa caccaagtac
     5821 gatgagaacg ataagttgat cagggaagtg aaggttatca ccctcaagtc aaagctcgtg
     5881 tctgatttca gaaaggattt ccaattctac aaggtgaggg aaatcaacaa ctaccaccac
     5941 gctcacgatg cttaccttaa cgctgttgtt ggaaccgctc tcatcaagaa gtatcctaag
     6001 ctcgagtcag agttcgtgta cggtgattac aaggtgtacg atgtgaggaa gatgatcgct
     6061 aagtctgagc aagagatcgg aaaggctacc gctaagtatt tcttctactc taacatcatg
     6121 aatttcttca agaccgagat taccctcgct aacggtgaga tcagaaagag gccactcatc
     6181 gagacaaacg gtgaaacagg tgagatcgtg tgggataagg gaagggattt cgctaccgtt
     6241 agaaaggtgc tctctatgcc acaggtgaac atcgttaaga aaaccgaggt gcagaccggt
     6301 ggattctcta aagagtctat cctccctaag aggaactctg ataagctcat tgctaggaag
     6361 aaggattggg accctaagaa atacggtggt ttcgattctc ctaccgtggc ttactctgtt
     6421 ctcgttgtgg ctaaggttga gaagggaaag agtaagaagc tcaagtctgt taaggaactt
     6481 ctcggaatca ctatcatgga aaggtcatct ttcgagaaga acccaatcga tttcctcgag
     6541 gctaagggat acaaagaggt taagaaggat ctcatcatca agctcccaaa gtactcactc
     6601 ttcgaactcg agaacggtag aaagaggatg ctcgcttctg ctggtgagct tcaaaaggga
     6661 aacgagcttg ctctcccatc taagtacgtt aactttcttt acctcgcttc tcactacgag
     6721 aagttgaagg gatctccaga agataacgag cagaagcaac ttttcgttga gcagcacaag
     6781 cactacttgg atgagatcat cgagcagatc tctgagttct ctaaaagggt gatcctcgct
     6841 gatgcaaacc tcgataaggt gttgtctgct tacaacaagc acagagataa gcctatcagg
     6901 gaacaggcag agaacatcat ccatctcttc acccttacca acctcggtgc tcctgctgct
     6961 ttcaagtact tcgatacaac catcgatagg aagagataca cctctaccaa agaagtgctc
     7021 gatgctaccc tcatccatca gtctatcact ggactctacg agactaggat cgatctctca
     7081 cagctcggtg gtgattcaag ggctgatcct aagaagaaga ggaaggtttg acgtcgacga
     7141 tatgaagatg aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg cttaagtttg
     7201 tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt tttctaatat taaatgaatg
     7261 taagatcaca ttataatgaa taaacaaatg tttctataat ccattgtgaa tgttttgttg
     7321 gatctcttct gcagcatata actactgtat gtgctatggt atggactatg gaatatgatt
     7381 aaagataagc cagagctctg gtgacggacg tgggcaggtg tgatgaaaag ccttagggtc
     7441 aggtggcact tttcggggaa atgtgcgatg aacccctatt tgtttatttt tctaaataca
     7501 ttcaaatatg tatccgctaa tgagacaata accctgataa atgcttcaat tatattgaaa
     7561 aaggaagagt atgagtatcc aacatttccg tgtcgccctt attccctttt ttgcggcctt
     7621 ctgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7681 gttgggtgcc cgagtgggtt acattgaact ggatctcaac agcggcaaaa tcttagagag
     7741 ttttcgcccc gaagaacgct tcccaatgat gagcactttc aaagttctgc tatgtggcgc
     7801 ggtgttatcc cgtattgatg ctgggcaaga gcaactcggt cgccgcatac actattctca
     7861 gaatgacttg gttgagtatt caccagtcac agaaaagcat cttacggatg gcatgacagt
     7921 aagagaatta tgtagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7981 gacaacgatt ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8041 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8101 caccacgatg cctgtagcaa tggcaacaac tctccgcaaa ctattaactg gcgaactact
     8161 tactcttgct tcacgccagc aactcattga ctggatggag gcggacaaag ttgcaggacc
     8221 acttctgcgc tcggcacttc cggctggctg gtttattgct gataaatctg gagccggtga
     8281 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8341 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8401 gataggtgcc tcactgatta agcattggta actgtcagac gaagggaaat aaatagtagc
     8461 ccgcctgatg tgcgggcttt tttttggtgg tggttaccag tgtcgcttag ctaccgtagg
     8521 tcctcttagg cggtccgatt agcgacatag tcatcaat
//
