LOCUS       sequence-181781-        5322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: AtUbi10, Gene: TALEN_1 backbone with Esp3I ccdb 
            cassette for repeat cloning, Terminator: none (ends with P2A to be 
            fused to TALEN_2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A1002
FEATURES             Location/Qualifiers
     source          1..5322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             2230..2295
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2302..2322
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2761..3066)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2863..2882)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     promoter        4230..4334
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgccgt cgagctgcag gtcaacggat
      901 caggatattc ttgtttaaga tgttgaactc tatggaggtt tgtatgaact gatgatctag
      961 gaccggataa gttcccttct tcatagcgaa cttattcaaa gaatgttttg tgtatcattc
     1021 ttgttacatt gttattaatg aaaaaatatt attggtcatt ggactgaaca cgagtgttaa
     1081 atatggacca ggccccaaat aagatccatt gatatatgaa ttaaataaca agaataaatc
     1141 gagtcaccaa accacttgcc ttttttaacg agacttgttc accaacttga tacaaaagtc
     1201 attatcctat gcaaatcaat aatcatacaa aaatatccaa taacactaaa aaattaaaag
     1261 aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact
     1321 gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat
     1381 aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc
     1441 aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa
     1501 aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttagaaa
     1561 ttgtggttgt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg
     1621 agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta
     1681 gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta
     1741 ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt
     1801 ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa
     1861 tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta
     1921 ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt
     1981 ttagattctg ttaatcttag atcgaacacg attttctggg tttgatcgtt agatatcatc
     2041 ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg
     2101 tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg
     2161 tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acaggcctgc aggaaaaatg
     2221 gcttcctccg attataagga tcacgatggc gactacaagg accacgatat tgactacaaa
     2281 gacgatgacg ataaaatggc tcctaagaaa aagcgcaaag tcggtatcca tggcgttccc
     2341 tctagaatgg tggatctacg cacgctcggc tacagtcagc agcagcaaga gaagatcaaa
     2401 ccgaaggtgc gttcgacagt ggcgcagcac cacgaggcac tggtgggcca tgggtttaca
     2461 cacgcgcaca tcgttgcgct cagccaacac ccggcagcgt tagggaccgt cgctgtcacg
     2521 tatcagcaca taatcacggc gttgccagag gcgacacacg aagacatcgt tggcgtcggc
     2581 aaacagtggt ccggcgcacg cgccctggag gccttgctca cggatgcggg ggagttgaga
     2641 ggtccgccgt tacagttgga cacaggccaa cttgtgaaga ttgcaaaacg tggcggcgtg
     2701 accgcaatgg aggcagtgca tgccagcagg aatgcgttaa caggtgcccc cctggagacg
     2761 ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag
     2821 atcagccact tcttccccga taacggaaac cggcacactg gccatatcgg tggtcatcat
     2881 gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga
     2941 cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc
     3001 actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa
     3061 ctgcatttca ccagcccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg
     3121 cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg
     3181 cttcattctg catggttgtg cttaccagac cggagatatt gacatcatat atgccttgag
     3241 caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag catacctcct
     3301 tttgacatac ttcgggtata catatcagta tatattctta taccgcaaaa atcagcgcgc
     3361 aaatacgcat actgttatct ggcttcgtct ccaacgacca cctcgtcgcc ttggcctgcc
     3421 tcggcggacg tcctgccatg gatgcagtga aaaagggatt gccgcacgcg ccggaattga
     3481 tcagaagagt caatcgccgt attggcgaac gcacgtccca tcgcgttgcc ggatcccagc
     3541 tggtgaagtc cgagctggaa gaaaaaaaga gcgagctgcg ccacaagctc aagtacgtgc
     3601 cccacgagta catcgagctg atcgagatcg cccgcaacag cacccaagac cgcatcctgg
     3661 agatgaaagt gatggagttc ttcatgaagg tgtacggcta ccgcggcaag cacctgggcg
     3721 gctcccgcaa gcccgatggc gccatctaca ccgtgggctc ccccatcgac tatggcgtca
     3781 ttgtcgacac caaggcctac tccggcggct acaacttacc catcggtcag gccgacgaga
     3841 tgcaacgcta cgtgaaggag aaccagaccc gcaataagca cattaatccc aacgagtggt
     3901 ggaaggtgta cccctcctcc gtgaccgagt tcaaattcct gttcgtgtcc ggccacttca
     3961 agggcaatta taaggcccaa ctgacccgcc tgaaccgcaa gaccaactgc aacggcgccg
     4021 tgctgtccgt ggaggaactg ctgatcggcg gcgagatgat caaggctggt accctgaccc
     4081 tggaagaggt gcgccgcaag ttcaacaatg gtgaaatcaa tttcaggtcc ggaagcggcg
     4141 ccacgaactt ctctctgtta aagcaagcag gggacgtggg caggtgatga aaagccttag
     4201 ggtcaggtgg cacttttcgg ggaaatgtgc gatgaacccc tatttgttta tttttctaaa
     4261 tacattcaaa tatgtatccg ctaatgagac aataaccctg ataaatgctt caattatatt
     4321 gaaaaaggaa gagtatgagt atccaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4381 ccttctgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4441 atcagttggg tgcccgagtg ggttacattg aactggatct caacagcggc aaaatcttag
     4501 agagttttcg ccccgaagaa cgcttcccaa tgatgagcac tttcaaagtt ctgctatgtg
     4561 gcgcggtgtt atcccgtatt gatgctgggc aagagcaact cggtcgccgc atacactatt
     4621 ctcagaatga cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga
     4681 cagtaagaga attatgtagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4741 ttctgacaac gattggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4801 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4861 gtgacaccac gatgcctgta gcaatggcaa caactctccg caaactatta actggcgaac
     4921 tacttactct tgcttcacgc cagcaactca ttgactggat ggaggcggac aaagttgcag
     4981 gaccacttct gcgctcggca cttccggctg gctggtttat tgctgataaa tctggagccg
     5041 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5101 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5161 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agacgaaggg aaataaatag
     5221 tagcccgcct gatgtgcggg cttttttttg gtggtggtta ccagtgtcgc ttagctaccg
     5281 taggtcctct taggcggtcc gattagcgac atagtcatca at
//
