LOCUS       sequence-181782-        5979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: ZmUbi, Gene: TALEN_1 backbone with Esp3I ccdb 
            cassette for repeat cloning, Terminator: none (ends with P2A to be 
            fused to TALEN_2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A1010
FEATURES             Location/Qualifiers
     source          1..5979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2009..2074
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2081..2101
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2540..2845)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2642..2661)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     promoter        4009..4113
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5181..5769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5670..5689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5923..5940
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga
      721 cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg
      781 gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg
      841 ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt
      901 tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac
      961 ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc ccccccccct ctctaccttc
     1021 tctagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg ttcatgtttg
     1081 tgttagatcc gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg
     1141 tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga atcctgggat
     1201 ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg
     1261 gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc
     1321 ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag
     1381 atcggagtag aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt
     1441 gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg
     1501 ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc
     1561 ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa
     1621 tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca
     1681 tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt
     1741 gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct
     1801 aaccttgagt acctatctat tataataaac aagtatgttt tataattatt ttgatcttga
     1861 tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc ctgccttcat
     1921 acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt
     1981 acttcctgca ggaaaaatgg cttcctccga ttataaggat cacgatggcg actacaagga
     2041 ccacgatatt gactacaaag acgatgacga taaaatggct cctaagaaaa agcgcaaagt
     2101 cggtatccat ggcgttccct ctagaatggt ggatctacgc acgctcggct acagtcagca
     2161 gcagcaagag aagatcaaac cgaaggtgcg ttcgacagtg gcgcagcacc acgaggcact
     2221 ggtgggccat gggtttacac acgcgcacat cgttgcgctc agccaacacc cggcagcgtt
     2281 agggaccgtc gctgtcacgt atcagcacat aatcacggcg ttgccagagg cgacacacga
     2341 agacatcgtt ggcgtcggca aacagtggtc cggcgcacgc gccctggagg ccttgctcac
     2401 ggatgcgggg gagttgagag gtccgccgtt acagttggac acaggccaac ttgtgaagat
     2461 tgcaaaacgt ggcggcgtga ccgcaatgga ggcagtgcat gccagcagga atgcgttaac
     2521 aggtgccccc ctggagacgt tatattcccc agaacatcag gttaatggcg tttttgatgt
     2581 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggaaacc ggcacactgg
     2641 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
     2701 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
     2761 cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
     2821 tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg
     2881 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
     2941 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg
     3001 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct
     3061 gcttcatagc atacctcctt ttgacatact tcgggtatac atatcagtat atattcttat
     3121 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttcgtctc caacgaccac
     3181 ctcgtcgcct tggcctgcct cggcggacgt cctgccatgg atgcagtgaa aaagggattg
     3241 ccgcacgcgc cggaattgat cagaagagtc aatcgccgta ttggcgaacg cacgtcccat
     3301 cgcgttgccg gatcccagct ggtgaagtcc gagctggaag aaaaaaagag cgagctgcgc
     3361 cacaagctca agtacgtgcc ccacgagtac atcgagctga tcgagatcgc ccgcaacagc
     3421 acccaagacc gcatcctgga gatgaaagtg atggagttct tcatgaaggt gtacggctac
     3481 cgcggcaagc acctgggcgg ctcccgcaag cccgatggcg ccatctacac cgtgggctcc
     3541 cccatcgact atggcgtcat tgtcgacacc aaggcctact ccggcggcta caacttaccc
     3601 atcggtcagg ccgacgagat gcaacgctac gtgaaggaga accagacccg caataagcac
     3661 attaatccca acgagtggtg gaaggtgtac ccctcctccg tgaccgagtt caaattcctg
     3721 ttcgtgtccg gccacttcaa gggcaattat aaggcccaac tgacccgcct gaaccgcaag
     3781 accaactgca acggcgccgt gctgtccgtg gaggaactgc tgatcggcgg cgagatgatc
     3841 aaggctggta ccctgaccct ggaagaggtg cgccgcaagt tcaacaatgg tgaaatcaat
     3901 ttcaggtccg gaagcggcgc cacgaacttc tctctgttaa agcaagcagg ggacgtgggc
     3961 aggtgatgaa aagccttagg gtcaggtggc acttttcggg gaaatgtgcg atgaacccct
     4021 atttgtttat ttttctaaat acattcaaat atgtatccgc taatgagaca ataaccctga
     4081 taaatgcttc aattatattg aaaaaggaag agtatgagta tccaacattt ccgtgtcgcc
     4141 cttattccct tttttgcggc cttctgcctt cctgtttttg ctcacccaga aacgctggtg
     4201 aaagtaaaag atgctgaaga tcagttgggt gcccgagtgg gttacattga actggatctc
     4261 aacagcggca aaatcttaga gagttttcgc cccgaagaac gcttcccaat gatgagcact
     4321 ttcaaagttc tgctatgtgg cgcggtgtta tcccgtattg atgctgggca agagcaactc
     4381 ggtcgccgca tacactattc tcagaatgac ttggttgagt attcaccagt cacagaaaag
     4441 catcttacgg atggcatgac agtaagagaa ttatgtagtg ctgccataac catgagtgat
     4501 aacactgcgg ccaacttact tctgacaacg attggaggac cgaaggagct aaccgctttt
     4561 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4621 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aactctccgc
     4681 aaactattaa ctggcgaact acttactctt gcttcacgcc agcaactcat tgactggatg
     4741 gaggcggaca aagttgcagg accacttctg cgctcggcac ttccggctgg ctggtttatt
     4801 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4861 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4921 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4981 gacgaaggga aataaatagt agcccgcctg atgtgcgggc ttttttttgg tggtggttac
     5041 cagtgtcgct tagctaccgt aggtcctctt aggcggtccg attagcgaca tagtcatcaa
     5101 tgccttgacg gcaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5161 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5221 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct actaactctt
     5281 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5341 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5401 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5461 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgctcacag
     5521 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5581 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5641 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5701 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5761 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5821 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5881 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5941 gaagcggaag agcgcccacc tgctcatgat cggcgcgcc
//
