LOCUS       sequence-181785-        8476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A1110
FEATURES             Location/Qualifiers
     source          1..8476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             2893..6996
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2893..6996
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6997..7044
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      7056..7304
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        7384..7488
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc tgcaggcgcg cctgcagtgc agcgtgaccc
      901 ggtcgtgccc ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac
      961 atattttttt tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa
     1021 actttactct acgaataata taatctatag tactacaata atatcagtgt tttagagaat
     1081 catataaatg aacagttaga catggtctaa aggacaattg agtattttga caacaggact
     1141 ctacagtttt atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc
     1201 tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt
     1261 tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa
     1321 ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa
     1381 gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc
     1441 ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa
     1501 ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg
     1561 ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca
     1621 tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc
     1681 ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc
     1741 ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt
     1801 tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt
     1861 caaggtacgc cgctcgtcct cccccccccc cctctctacc ttctctagat cggcgttccg
     1921 gtccatggtt agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg
     1981 tgttagatcc gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct
     2041 gattgctaac ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc
     2101 agacgggatc gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt
     2161 cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt
     2221 gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattaat
     2281 tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat
     2341 attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg
     2401 ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga
     2461 tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac
     2521 tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac
     2581 gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta
     2641 ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc
     2701 tatctattat aataaacaag tatgttttat aattattttg atcttgatat acttggatga
     2761 tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt
     2821 gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact tcctgcaggg
     2881 cgatctattc gaatggacaa gaagtactcg atcggcctcg acatcgggac gaactcagtt
     2941 ggctgggccg tgatcaccga cgagtacaag gtgccctcta agaagttcaa ggtcctgggg
     3001 aacaccgacc gccattccat caagaagaac ctcatcggcg ctctcctgtt cgacagcggg
     3061 gagaccgctg aggctacgag gctcaagaga accgctaggc gccggtacac gagaaggaag
     3121 aacaggatct gctacctcca agagattttc tccaacgaga tggccaaggt tgacgattca
     3181 ttcttccacc gcctggagga gtctttcctc gtggaggagg ataagaagca cgagcggcat
     3241 cccatcttcg gcaacatcgt ggacgaggtt gcctaccacg agaagtaccc tacgatctac
     3301 catctgcgga agaagctcgt ggactccacc gataaggcgg acctcagact gatctacctc
     3361 gctctggccc acatgatcaa gttccgcggc catttcctga tcgaggggga tctcaaccca
     3421 gacaacagcg atgttgacaa gctgttcatc caactcgtgc agacctacaa ccaactcttc
     3481 gaggagaacc cgatcaacgc ctctggcgtg gacgcgaagg ctatcctgtc cgcgaggctc
     3541 tcgaagtcca ggaggctgga gaacctgatc gctcagctcc caggcgagaa gaagaacggc
     3601 ctgttcggga acctcatcgc tctcagcctg gggctcaccc cgaacttcaa gtcgaacttc
     3661 gatctcgctg aggacgccaa gctgcaactc tccaaggaca cctacgacga tgacctcgat
     3721 aacctcctgg cccagatcgg cgatcaatac gcggacctgt tcctcgctgc caagaacctg
     3781 tcggacgcca tcctcctgtc agatatcctc cgcgtgaaca ccgagatcac gaaggctcca
     3841 ctctctgcct ccatgatcaa gcgctacgac gagcaccatc aggatctgac cctcctgaag
     3901 gcgctggtcc gccaacagct cccggagaag tacaaggaga ttttcttcga tcagtcgaag
     3961 aacggctacg ctgggtacat cgacggcggg gcctcacaag aggagttcta caagttcatc
     4021 aagccaatcc tggagaagat ggacggcacg gaggagctcc tggtgaagct caacagggag
     4081 gacctcctgc ggaagcagag aaccttcgat aacggcagca tcccccacca aatccatctc
     4141 ggggagctgc acgccatcct gagaaggcaa gaggacttct accctttcct caaggataac
     4201 cgggagaaga tcgagaagat cctgaccttc agaatcccat actacgtcgg ccctctcgcg
     4261 cgggggaact caagattcgc ttggatgacc cgcaagtctg aggagaccat cacgccgtgg
     4321 aacttcgagg aggtggtgga caagggcgct agcgctcagt cgttcatcga gaggatgacc
     4381 aacttcgaca agaacctgcc caacgagaag gtgctcccta agcactcgct cctgtacgag
     4441 tacttcaccg tctacaacga gctcacgaag gtgaagtacg tcaccgaggg catgcgcaag
     4501 ccagcgttcc tgtccgggga gcagaagaag gctatcgtgg acctcctgtt caagaccaac
     4561 cggaaggtca cggttaagca actcaaggag gactacttca agaagatcga gtgcttcgat
     4621 tcggtcgaga tcagcggcgt tgaggaccgc ttcaacgcca gcctcgggac ctaccacgat
     4681 ctcctgaaga tcatcaagga taaggacttc ctggacaacg aggagaacga ggatatcctg
     4741 gaggacatcg tgctgaccct cacgctgttc gaggacaggg agatgatcga ggagcgcctg
     4801 aagacgtacg cccatctctt cgatgacaag gtcatgaagc aactcaagcg ccggagatac
     4861 accggctggg ggaggctgtc ccgcaagctc atcaacggca tccgggacaa gcagtccggg
     4921 aagaccatcc tcgacttcct caagagcgat ggcttcgcca acaggaactt catgcaactg
     4981 atccacgatg acagcctcac cttcaaggag gatatccaaa aggctcaagt gagcggccag
     5041 ggggactcgc tgcacgagca tatcgcgaac ctcgctggct cccccgcgat caagaagggc
     5101 atcctccaga ccgtgaaggt tgtggacgag ctcgtgaagg tcatgggccg gcacaagcct
     5161 gagaacatcg tcatcgagat ggccagagag aaccaaacca cgcagaaggg gcaaaagaac
     5221 tctagggagc gcatgaagcg catcgaggag ggcatcaagg agctggggtc ccaaatcctc
     5281 aaggagcacc cagtggagaa cacccaactg cagaacgaga agctctacct gtactacctc
     5341 cagaacggca gggatatgta cgtggaccaa gagctggata tcaaccgcct cagcgattac
     5401 gacgtcgatc atatcgttcc ccagtctttc ctgaaggatg actccatcga caacaaggtc
     5461 ctcaccaggt cggacaagaa ccgcggcaag tcagataacg ttccatctga ggaggtcgtt
     5521 aagaagatga agaactactg gaggcagctc ctgaacgcca agctgatcac gcaaaggaag
     5581 ttcgacaacc tcaccaaggc tgagagaggc gggctctcag agctggacaa ggccggcttc
     5641 atcaagcggc agctggtcga gaccagacaa atcacgaagc acgttgcgca aatcctcgac
     5701 tctcggatga acacgaagta cgatgagaac gacaagctga tcagggaggt taaggtgatc
     5761 accctgaagt ctaagctcgt ctccgacttc aggaaggatt tccagttcta caaggttcgc
     5821 gagatcaaca actaccacca tgcccatgac gcttacctca acgctgtggt cggcaccgct
     5881 ctgatcaaga agtacccaaa gctggagtcc gagttcgtgt acggggacta caaggtttac
     5941 gatgtgcgca agatgatcgc caagtcggag caagagatcg gcaaggctac cgccaagtac
     6001 ttcttctact caaacatcat gaacttcttc aagaccgaga tcacgctggc caacggcgag
     6061 atccggaaga gaccgctcat cgagaccaac ggcgagacgg gggagatcgt gtgggacaag
     6121 ggcagggatt tcgcgaccgt ccgcaaggtt ctctccatgc cccaggtgaa catcgtcaag
     6181 aagaccgagg tccaaacggg cgggttctca aaggagtcta tcctgcctaa gcggaacagc
     6241 gacaagctca tcgccagaaa gaaggactgg gacccaaaga agtacggcgg gttcgacagc
     6301 cctaccgtgg cctactcggt cctggttgtg gcgaaggttg agaagggcaa gtccaagaag
     6361 ctcaagagcg tgaaggagct cctggggatc accatcatgg agaggtccag cttcgagaag
     6421 aacccaatcg acttcctgga ggccaagggc tacaaggagg tgaagaagga cctgatcatc
     6481 aagctcccga agtactctct cttcgagctg gagaacggca ggaagagaat gctggcttcc
     6541 gctggcgagc tccagaaggg gaacgagctc gcgctgccaa gcaagtacgt gaacttcctc
     6601 tacctggctt cccactacga gaagctcaag ggcagcccgg aggacaacga gcaaaagcag
     6661 ctgttcgtcg agcagcacaa gcattacctc gacgagatca tcgagcaaat ctccgagttc
     6721 agcaagcgcg tgatcctcgc cgacgcgaac ctggataagg tcctctccgc ctacaacaag
     6781 caccgggaca agcccatcag agagcaagcg gagaacatca tccatctctt caccctgacg
     6841 aacctcggcg ctcctgctgc tttcaagtac ttcgacacca cgatcgatcg gaagagatac
     6901 acctccacga aggaggtcct ggacgcgacc ctcatccacc agtcgatcac cggcctgtac
     6961 gagacgagga tcgacctctc acaactcggc ggggataaga gacccgcagc aaccaagaag
     7021 gcagggcaag caaagaagaa gaagtgactc gagtatatga agatgaagat gaaatatttg
     7081 gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt
     7141 atgaatttgt ggctttttct aatattaaat gaatgtaaga tcacattata atgaataaac
     7201 aaatgtttct ataatccatt gtgaatgttt tgttggatct cttctgcagc atataactac
     7261 tgtatgtgct atggtatgga ctatggaata tgattaaaga taaggagctc gttaaccggt
     7321 gacggacgtg ggcaggtgtg atgaaaagcc ttagggtcag gtggcacttt tcggggaaat
     7381 gtgcgatgaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctaatg
     7441 agacaataac cctgataaat gcttcaatta tattgaaaaa ggaagagtat gagtatccaa
     7501 catttccgtg tcgcccttat tccctttttt gcggccttct gccttcctgt ttttgctcac
     7561 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcccg agtgggttac
     7621 attgaactgg atctcaacag cggcaaaatc ttagagagtt ttcgccccga agaacgcttc
     7681 ccaatgatga gcactttcaa agttctgcta tgtggcgcgg tgttatcccg tattgatgct
     7741 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtattca
     7801 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg tagtgctgcc
     7861 ataaccatga gtgataacac tgcggccaac ttacttctga caacgattgg aggaccgaag
     7921 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7981 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8041 gcaacaactc tccgcaaact attaactggc gaactactta ctcttgcttc acgccagcaa
     8101 ctcattgact ggatggaggc ggacaaagtt gcaggaccac ttctgcgctc ggcacttccg
     8161 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8221 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8281 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8341 cattggtaac tgtcagacga agggaaataa atagtagccc gcctgatgtg cgggcttttt
     8401 tttggtggtg gttaccagtg tcgcttagct accgtaggtc ctcttaggcg gtccgattag
     8461 cgacatagtc atcaat
//
