LOCUS       sequence-181800-        3868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: 35S, Gene: GFP, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A3001
FEATURES             Location/Qualifiers
     source          1..3868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1366..1711
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1654..1674
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(1762..1790)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2385..2405
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2456..2704
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        2776..2880
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgccag atttgccttt tcaatttcag
      901 aaagaatgct aacccacaga tggttagaga ggcttacgca gcaggtatca tcaagacgat
      961 ctacccgagc aataatctcc aggaaatcaa ataccttccc aagaaggtta aagatgcagt
     1021 caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag
     1081 aagtactatt ccagtatgga cgattcaagg cttgcttcac aaaccaaggc aagtaataga
     1141 gattggagtc tctaaaaagg tagttcccac tgaatcaaag gccatggagt caaagattca
     1201 aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt
     1261 acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta
     1321 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca
     1381 aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt
     1441 gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
     1501 catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
     1561 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
     1621 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
     1681 ataaggaagt tcatttcatt tggagagaac acgggggact cctgcaggat ggtgagtaaa
     1741 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     1801 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
     1861 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaaccct ggtcaccacc
     1921 ctgacctacg gcgtgcagtg cttctcccgt taccctgatc atatgaagcg gcacgacttc
     1981 ttcaagagcg ccatgcctga gggatacgtg caggagagga ccatcttctt caaggacgac
     2041 gggaactaca agacacgtgc tgaagtcaag tttgagggag acaccctcgt caacaggatc
     2101 gagcttaagg gaatcgattt caaggaggac ggaaacatcc tcggccacaa gttggaatac
     2161 aactacaact cccacaacgt atacatcatg gccgacaagc aaaagaacgg catcaaagcc
     2221 aacttcaaga cccgccacaa catcgaagac ggcggcgtgc aactcgctga tcattatcaa
     2281 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     2341 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     2401 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataact cgagatatga
     2461 agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt
     2521 ttttcttggc ttgttgtgtt atgaatttgt ggctttttct aatattaaat gaatgtaaga
     2581 tcacattata atgaataaac aaatgtttct ataatccatt gtgaatgttt tgttggatct
     2641 cttctgcagc atataactac tgtatgtgct atggtatgga ctatggaata tgattaaaga
     2701 taaggagctc cggtgacgga cgtgggcagg tgatgaaaag ccttagggtc aggtggcact
     2761 tttcggggaa atgtgcgatg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2821 tatccgctaa tgagacaata accctgataa atgcttcaat tatattgaaa aaggaagagt
     2881 atgagtatcc aacatttccg tgtcgccctt attccctttt ttgcggcctt ctgccttcct
     2941 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcc
     3001 cgagtgggtt acattgaact ggatctcaac agcggcaaaa tcttagagag ttttcgcccc
     3061 gaagaacgct tcccaatgat gagcactttc aaagttctgc tatgtggcgc ggtgttatcc
     3121 cgtattgatg ctgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3181 gttgagtatt caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3241 tgtagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatt
     3301 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3361 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3421 cctgtagcaa tggcaacaac tctccgcaaa ctattaactg gcgaactact tactcttgct
     3481 tcacgccagc aactcattga ctggatggag gcggacaaag ttgcaggacc acttctgcgc
     3541 tcggcacttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3601 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3661 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3721 tcactgatta agcattggta actgtcagac gaagggaaat aaatagtagc ccgcctgatg
     3781 tgcgggcttt tttttggtgg tggttaccag tgtcgcttag ctaccgtagg tcctcttagg
     3841 cggtccgatt agcgacatag tcatcaat
//
