LOCUS       sequence-181801-        3708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 terminator trpC gene Aspergillus nidulans between FS3 and FS4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB1_T_34_ani_trpC
FEATURES             Location/Qualifiers
     source          1..3708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(140..159)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             151..966
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             151..966
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(224..243)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1051..1639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1540..1559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1793..1810
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1927..1948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1963..1968,1969..1986,1987..1993)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2001..2017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2006..2028
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2025..2041
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2025..2041
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2060..2079
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2060..2078
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2148..2921)
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     promoter        complement(3016..3034)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3017..3034)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3048..3065)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3048..3064)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3057..3079)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3205..3660)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3342..3363)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3554..3573
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gatctctgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
       61 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaacaat aaaactgtct
      121 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc
      181 tctaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
      241 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
      301 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
      361 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
      421 cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta
      481 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
      541 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
      601 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
      661 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
      721 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
      781 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
      841 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
      901 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
      961 ttctaagaat taattcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1021 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1081 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1141 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1201 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1261 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1321 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1381 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1441 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1501 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1561 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1621 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1681 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     1741 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     1801 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     1861 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     1921 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     1981 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2041 ccatgattac gccaagctct aatacgactc actataggga aagctcggta ccacgcatgc
     2101 tgcagacgcg ttacgtatcg gatccagaat tcgtgatgaa gacgcagcga gtggagatgt
     2161 ggagtgggcg cttacacagt acacgaggac ttctagaaag aaggattacc tctaaacaag
     2221 tgtacctgtg cattctgggt aaacgactca taggagagtt gtaaaaaagt ttcggccggc
     2281 gtattgggtg ttacggagca ttcactaggc aaccatgcat ggttactatt gtatacccat
     2341 cttagtagga atgattttcg aggtttatac ctacgatgaa tgtgtgtcct gtaggcttga
     2401 gagttcaagg aagaaacagt gcaattatct ttgcgaaccc aggggctggt gacggaattt
     2461 tcatagtcaa gctatcagag taaagaagag gagcatgtca aagtacaatt agagacaaat
     2521 atatagtcgc gtggagccaa gagcggattc ctcagtctcg taggactctt gacgaccgtt
     2581 gatctgcttg atctcgtctc ccgaaaatga aaatagactc tgctaagcta ttcttctgct
     2641 tcgccggagc ctgaagggcg tactagggtt gcgaggtcca atgcattaat gcattgcaga
     2701 tgagctgtat ctggaagagg taaacccgaa acgcgtttta ttcttgttga catggagcta
     2761 ttaaatcact agaaggcact ctttgctgct tggacaaatg aacgtatctt atcgagatcc
     2821 tgaacaccat ttgtctcaac tccggagctg acatcgacac caacgatctt atatccagat
     2881 tcgtcaagct gtttgatgat ttcagtaacg ttaagtggat caagcgcgtc ttcatctgaa
     2941 ttcgtcgaca agcttctcga gcctaggcta gctctagacc acacgtgtgg gggcccgagc
     3001 tcgcggccgc tgtattctat agtgtcacct aaatggccgc acaattcact ggccgtcgtt
     3061 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     3121 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3181 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
     3241 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     3301 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     3361 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3421 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     3481 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     3541 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     3601 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     3661 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttaa
//
