LOCUS       sequence-181802-        4812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with AMA1_2.8 sequence for transient plasmid propagation in A. 
            niger between FS 34.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB1_O_34_AMA1_2.8
FEATURES             Location/Qualifiers
     source          1..4812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      138..165
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(177..764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(257..276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     804..821
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     805..821
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3912..3928)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3912..3928)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      3976..4007
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        4008..4110
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(4111..4812,1..114)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4184..4203)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
       61 aatcctgata tgaataaatt gcagtttcac ttgatgctcg atgagttttt ctaatgagga
      121 cctaaatgta atcacctggc tcaccttcgg gtgggccttt ctgcgttgct ggcgtttttc
      181 cataggctcc gcccccctga cgagcatcac aaaaatcgat gctcaagtca gaggtggcga
      241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      601 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttacctcg
      661 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      721 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgattt
      781 tctaccgaag aaaggcccac ccgtgtaaaa cgacggccag tttatctagt cagcttgatt
      841 ctagctgatc gtggaccgga aggtgagcca gtgagttgat tgcagtccag ttacgctgga
      901 gtctgaggct cgtcctgaat gatatgcggc ctcgaattcg aagacgcgct tatgctgcag
      961 acgcgttacg tatcggatcc agaattcgtg atatctgaat tcgtcgacaa gcttattttt
     1021 tgtatactgt tttgtgatag cacgaagttt ttccacggta tcttgttaaa aatatatatt
     1081 tgtggcgggc ttacctacat caaattaata agagactaat tataaactaa acacacaagc
     1141 aagctacttt agggtaaaag tttataaatg cttttgacgt ataaacgttg cttgtattta
     1201 ttattacaat taaaggtgga tagaaaacct agagactagt tagaaactaa tctcaggttt
     1261 gcgttaaact aaatcagagc ccgagaggtt aacagaacct agaaggggac tagatatccg
     1321 ggtagggaaa caaaaaaaaa aaacaagaca gccacatatt agggagacta gttagaagct
     1381 agttccagga ctaggaaaat aaaagacaat gataccacag tctagttgac aactagatag
     1441 attctagatt gaggccaaag tctctgagat ccaggttagt tgcaactaat actagttagt
     1501 atctagtctc ctataactct gaagctagaa taacttacta ctattatcct caccactgtt
     1561 cagctgcgca aacggagtga ttgcaaggtg ttcagagact agttattgac tagtcagtga
     1621 ctagcaataa ctaacaaggt attaacctac catgtctgcc atcaccctgc acttcctcgg
     1681 gctcagcagc cttttcctcc tcattttcat gctcattttc cttgtttaag actgtgacta
     1741 gtcaaagact agtccagaac cacaaaggag aaatgtctta ccactttctt cattgcttgt
     1801 ctcttttgca ttatccatgt ctgcaactag ttagagtcta gttagtgact agtccgacga
     1861 ggacttgctt gtctccggat tgttggagga actctccagg gcctcaagat ccacaacaga
     1921 gccttctaga tgactggtca ataactagtt ggtctttgtc tgagtctgac ttacgaggtt
     1981 gcatactcgc tccctttgcc tcgtcaatcg atgagaaaaa gcgccaaaac tcgcaatatg
     2041 gctttgaacc acacggtgct gagactagtt agaatctagt cccaaactag cttggatagc
     2101 ttacctttgc cctttgcgtt gcgacaggtc ttgcagggta tggttccttt ctcaccagct
     2161 gatttagctg ccttgctacc ctcacggcgg atctgcataa agagtggcta gaggttataa
     2221 attagcactg atcctaggta cggggctgaa tgtaacttgc ctttcctttc tcatcgcgcg
     2281 gcaagacagg cttgctcaaa ttcctaccag tcacaggggt atgcacggcg tacggaccac
     2341 ttgaactagt cacagattag ttagcaacta gtctgcattg aatggctgta cttacgggcc
     2401 ctcgccattg tcctgatcat ttccagcttc accctcgttg ctgcaaagta gttagtgact
     2461 agtcaaggac tagttgaaat gggagaagaa actcacgaat tctcgacacc cttagtattg
     2521 tggtccttgg acttggtgct gctatatatt agctaataca ctagttagac tcacagaaac
     2581 ttacgcagct cgcttgcgct tcttggtagg agtcggggtt gggagaacag tgccttcaaa
     2641 caagccttca taccatgcta cttgactagt cagggactag tcaccaagta atctagatag
     2701 gacttgcctt tggcctccat cagttccttc atagtgggag gtccattgtg caatgtaaac
     2761 tccatgccgt gggagttctt gtccttcaag tgcttgacca atatgtttct gttggcagag
     2821 ggaacctgtc aactagttaa taactagtca gaaactagta tagcagtaga ctcactgtac
     2881 gcttgaggca tcccttcact cggcagtaga cttcatatgg atggatatca ggcacgccat
     2941 tgtcgtcctg tggactagtc agtaactagg cttaaagcta gtcgggtcgg cttactatct
     3001 tgaaatccgg cagcgtaagc tccccgtcct taactgcctc gagatagtga cagtactctg
     3061 gggactttcg gagatcgtta tcgcgaatgc tcggcatact aatcgttgac tagtcttgga
     3121 ctagtcccga gcaaaaagga ttggaggagg aggaggaagg tgagagtgag acaaagagcg
     3181 aaataagagc ttcaaaggct atctctaagc agtatgaagg ttaagtatct agttcttgac
     3241 tagatttaaa agagatttcg actagttatg tacctggagt ttggatatag gaatgtgttg
     3301 tggtaacgaa atgtaagggg gaggaaagaa aaagtcggtc aagaggtaac tctaagtcgg
     3361 ccattccttt ttgggaggcg ctaaccataa acggcatggt cgacttagag ttagctcagg
     3421 gaatttaggg agttatctgc gaccaccgag gaacggcgga atgccaaaga atcccgatgg
     3481 agctctagct ggcggttgac aaccccacct tttggcgttt ctgcggcgtt gcaggcggga
     3541 ctggatactt cgtagaacca gaaaggcaag gcagaacgcg ctcagcaaga gtgttggaag
     3601 tgatagcatg atgtgccttg ttaactaggt caaaatctgc agtatgcttg atgttatcca
     3661 aagtgtgaga gaggaaggtc caaacataca cgattgggag agggcctagg tataagagtt
     3721 tttgagtaga acgcatgtga gcccagccat ctcgaggaga ttaaacacgg gccggcattt
     3781 gatggctatg ttagtacccc aatggaaagc ctgagagtcc agtggcgctg cgtcttcgaa
     3841 ttcgcgcgtg atcttacggc attatacgta tgatcggtcc acgatcagct agattatcta
     3901 gtcagcttga tgtcatagct gtttcctgag gctcaatact gaccatttaa atcatacctg
     3961 acctccatag cagaaagtca aaagcctccg accggaggct tttgacttga tcggcacgta
     4021 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
     4081 tcgagatttt caggagctaa ggaagctaaa atgagccata ttcaacggga aacgtcttgc
     4141 ttgaagccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     4201 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
     4261 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     4321 aggctaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     4381 cctgatgatg catggttact caccactgcg atcccaggga aaacagcatt ccaggtatta
     4441 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     4501 ttgcattcga ttcctgtttg taattgtcct tttaacggcg atcgcgtatt tcgtctcgct
     4561 caggcgcaat cacgaatgaa taacggtttg gttggtgcga gtgattttga tgacgagcgt
     4621 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aactcttgcc attctcaccg
     4681 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     4741 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     4801 atcctatgga ac
//
