LOCUS       sequence-181804-        3491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: CmYLCV, Gene: GFP, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A3003
FEATURES             Location/Qualifiers
     source          1..3491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1385..1413)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2008..2028
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2079..2327
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        2399..2503
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgcctg gcagacatac tgtcccacaa
      901 atgaagatgg aatctgtaaa agaaaacgcg tgaaataatg cgtctgacaa aggttaggtc
      961 ggctgccttt aatcaatacc aaagtggtcc ctaccacgat ggaaaaactg tgcagtcggt
     1021 ttggcttttt ctgacgaaca aataagattc gtggccgaca ggtgggggtc caccatgtga
     1081 aggcatcttc agactccaat aatggagcaa tgacgtaagg gcttacgaaa taagtaaggg
     1141 tagtttggga aatgtccact cacccgtcag tctataaata cttagcccct ccctcattgt
     1201 taagggagca aaatctcaga gagatagtcc tagagagaga aagagagcaa gtagcctaga
     1261 agtagtcaag gcggcgaagt attcaggcac gtggccagga agaagaaaag ccaagacgac
     1321 gaaaacaggt aagagctaag cttcctgcag gatggtgagt aaaggagaag aacttttcac
     1381 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
     1441 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
     1501 tactggaaaa ctacctgttc catggccaac cctggtcacc accctgacct acggcgtgca
     1561 gtgcttctcc cgttaccctg atcatatgaa gcggcacgac ttcttcaaga gcgccatgcc
     1621 tgagggatac gtgcaggaga ggaccatctt cttcaaggac gacgggaact acaagacacg
     1681 tgctgaagtc aagtttgagg gagacaccct cgtcaacagg atcgagctta agggaatcga
     1741 tttcaaggag gacggaaaca tcctcggcca caagttggaa tacaactaca actcccacaa
     1801 cgtatacatc atggccgaca agcaaaagaa cggcatcaaa gccaacttca agacccgcca
     1861 caacatcgaa gacggcggcg tgcaactcgc tgatcattat caacaaaata ctccaattgg
     1921 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
     1981 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
     2041 tacacatggc atggatgaac tatacaaata actcgagata tgaagatgaa gatgaaatat
     2101 ttggtgtgtc aaataaaaag cttgtgtgct taagtttgtg tttttttctt ggcttgttgt
     2161 gttatgaatt tgtggctttt tctaatatta aatgaatgta agatcacatt ataatgaata
     2221 aacaaatgtt tctataatcc attgtgaatg ttttgttgga tctcttctgc agcatataac
     2281 tactgtatgt gctatggtat ggactatgga atatgattaa agataaggag ctccggtgac
     2341 ggacgtgggc aggtgatgaa aagccttagg gtcaggtggc acttttcggg gaaatgtgcg
     2401 atgaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc taatgagaca
     2461 ataaccctga taaatgcttc aattatattg aaaaaggaag agtatgagta tccaacattt
     2521 ccgtgtcgcc cttattccct tttttgcggc cttctgcctt cctgtttttg ctcacccaga
     2581 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcccgagtgg gttacattga
     2641 actggatctc aacagcggca aaatcttaga gagttttcgc cccgaagaac gcttcccaat
     2701 gatgagcact ttcaaagttc tgctatgtgg cgcggtgtta tcccgtattg atgctgggca
     2761 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt attcaccagt
     2821 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgtagtg ctgccataac
     2881 catgagtgat aacactgcgg ccaacttact tctgacaacg attggaggac cgaaggagct
     2941 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3001 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3061 aactctccgc aaactattaa ctggcgaact acttactctt gcttcacgcc agcaactcat
     3121 tgactggatg gaggcggaca aagttgcagg accacttctg cgctcggcac ttccggctgg
     3181 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3241 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3301 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3361 gtaactgtca gacgaaggga aataaatagt agcccgcctg atgtgcgggc ttttttttgg
     3421 tggtggttac cagtgtcgct tagctaccgt aggtcctctt aggcggtccg attagcgaca
     3481 tagtcatcaa t
//
