LOCUS       sequence-181820-       14177 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB3 for mttA integration with homologous flanks at pyrG locus, 
            hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 14177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: pMST694_BB3_pTet-on_mttA_trpC_pcoxA:pyrGtrunc_...
FEATURES             Location/Qualifiers
     source          1..14177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1701..1816
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(1834..2451,2452..2457,2458..2493,2494..2496,2497..2532,
                     2533..2535,2536..2571,2572..2580)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    join(2928..2946,2947..2969,2970..2988,2989..3011,3012..3030,
                     3031..3053,3054..3072,3073..3095,3096..3114,3115..3137,
                     3138..3156,3157..3179,3180..3198)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          3268..3383
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     terminator      4316..5089
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     regulatory      10136..10145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10729..11754
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             10729..11754
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(12417..12433)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12417..12433)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      12481..12512
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        12513..12615
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             12616..13431
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(12689..12708)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      13455..13482
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(13494..14081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13574..13593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14121..14138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14122..14138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aggtgagcca gtgagttgat tgcagtccag ttacgctgga gtctgaggct cgtcctgaat
       61 gatatgcggc ccgctgtagg tcaattgcga cttggaggaa gggatccgcc aaggatgctc
      121 tggatccgca tccggccgct cttgcgcccc atcaatcgcc cgactataaa tcgaactact
      181 ttcggcatct tctagacttc ctaataccgc ctagtcatag cagattcaag ctgagaacac
      241 cacaagtaaa tatcacccat catgcttacc ctgaccgtcc ctgaaaacta cgggtatgtg
      301 ccaattctac aattccttgc agacaatgcc attctcccca tgaagtctga tgctaactat
      361 cctgcagctc tgtcattgcc gtcgctctgg gtgccatccc cgtcctgagc ttcgtccatg
      421 gcgccgtcgt gtctcgtctc cgcaaggaag ctgattgccc ctaccctcac tgctatgcga
      481 ccgtagagca gtgcaagacc aacgtaagcc aacctcacac aaacaggatt cctcgagcta
      541 acatacattc cgaaccgtgc agcccaaggc cgagcagttc aactgcgctc agcgcgctca
      601 tgccaacttc cttgagaact ccagccaaac tatgctcttc ctcctggtag ctggactgaa
      661 gtacccccag ttggcgactg gcctcggaag catctgggtc ctcggtcgct cactgttcct
      721 ttacggatat gtgtactccg gcaagccgcg gggtcgcggt cgtttgtacg gcagcttcta
      781 cttgcttgca cagggagctc tctggggctt gacgtctttt ggagttgcga gggagttgat
      841 ttcctacttc taagtttgga ctgaatccgt ggtgtgattg aggtgattgg cgatgtttgg
      901 ctataccagc tatatgtaat aatctctact gtatactact attcaacgca ttttactatg
      961 cgtgctgcta gggtcggcaa tgacaatggc aatctgactg acgtggtcta tttctccatg
     1021 tgcagcaggg aatacgagct ccaatggacc tcgggagtgg cacagtcaat ggcaaggaaa
     1081 ctggagcgca aaaaaccccg cccctgacag ggcggggttt tttcgcgatc ggagtcgata
     1141 agcttcggag aatatggagc ttcatcgaat caccggcagt aagcgaagga gaatgtgaag
     1201 ccaggggtgt atagccgtcg gcgaaatagc atgccattaa cctaggtaca gaagtccaat
     1261 tgcttccgat ctggtaaaag attcacgaga tagtaccttc tccgaagtag gtagagcgag
     1321 tacccggcgc gtaagctccc taattggccc atccggcatc tgtagggcgt ccaaatatcg
     1381 tgcctctcct gctttgcccg gtgtatgaaa ccggaaaggc cgctcaggag ctggccagcg
     1441 gcgcagaccg ggaacacaag ctggcagtcg acccatccgg tgctctgcac tcgacctgct
     1501 gaggtccctc agtccctggt aggcagcttt gccccgtctg tccgcccggt gtgtcggcgg
     1561 ggttgacaag gtcgttgcgt cagtccaaca tttgttgcca tattttcctg ctctccccac
     1621 cagctgctct tttcttttct ctttcttttc ccatcttcag tatattcatc ttcccatcca
     1681 agaaccttta tttcccctaa gtaagtactt tgctacatcc atactccatc cttcccatcc
     1741 cttattcctt tgaacctttc agttcgagct ttcccacttc atcgcagctt gactaacagc
     1801 taccccgctt gagcagacat caccgaattc accatgtcta gactggacaa gagcaaagtc
     1861 ataaacggcg ctctggaatt actcaatgga gtcggtatcg aaggcctgac gacaaggaaa
     1921 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     1981 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     2041 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct
     2101 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     2161 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     2221 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     2281 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     2341 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     2401 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc
     2461 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     2521 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     2581 ctaagtaagg atccactagt acagcagaag aatctctctc cgctgttgct tcagtgtctg
     2641 ccatgcatta acttcatcct actgtcctac ccgcagtacc cattcacatt tgcggcagat
     2701 accaggttgt tttctattcc ctcggttctt cagttcttca gatattacat tactcgagaa
     2761 attggcggcg atgcaggagt ttggttggtt ttaattgttt cagtctcctt gacctgattt
     2821 gtatgacatg caatgtttcg acggatgact atctcggtga atatacgtca tgaatcatgc
     2881 ggccgcgcgt atcacgaggc cctttcgact tcactcgagt ttaccactcc ctatcagtga
     2941 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     3001 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     3061 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     3121 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     3181 ccctatcagt gatagagaaa agtgaaagtc gagctcccca tcttcagtat attcatcttc
     3241 ccatccaaga acctttattt cccctaagta agtactttgc tacatccata ctccatcctt
     3301 cccatccctt attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac
     3361 taacagctac cccgcttgag cagacatcac cgtttaaaca ccatggattc caagatccag
     3421 accaacgtcc ccctgcccaa ggcccccctc atccagaagg ctcgcggcaa gcgcaccaag
     3481 ggcatccccg ccctcgtcgc tggcgcttgt gccggtgccg tcgagatctc catcacctac
     3541 cccttcgagt ccgccaagac tcgtgctcag ctcaagcgcc gcaaccacga tgtcgccgcc
     3601 atcaagcccg gcatccgcgg ctggtacgct ggctacggcg ctactctcgt cggcaccacc
     3661 gtcaaggcct ccgtccagtt cgcctccttc aacatctacc gctccgccct ctccggcccc
     3721 aacggtgagc tgtccaccgg cgcctccgtc ctcgctggct tcggcgctgg cgtcaccgag
     3781 gccgtcctcg ccgtcacacc tgccgaagcc atcaagacca agatcatcga tgcccgcaag
     3841 gtcggcaacg ccgagctgag caccaccttc ggcgctatcg ccggcatcct ccgtgatcgt
     3901 ggccctctcg gcttcttctc cgccgtgggc cccaccatcc tccgccagtc ctccaacgcc
     3961 gccgtcaagt tcaccgtcta caacgagctg atcggcctcg cccgcaagta ctccaagaac
     4021 ggcgaggatg tccaccccct cgcctccacc ctggtcggct ccgtcaccgg cgtctgctgc
     4081 gcctggtcca cccagcccct cgatgtcatc aagacccgca tgcagtccct ccaggcccgc
     4141 cagctctacg gcaacacctt caactgcgtc aagaccctcc tgcgctccga gggcatcggc
     4201 gtgttctggt ccggcgtctg gttccgcacc ggtcgcctct ccctcacctc tgccatcatg
     4261 ttccccgttt acgagaaggt ctacaagttc ctcacccagc ccaactgatg agcttgatcc
     4321 acttaacgtt actgaaatca tcaaacagct tgacgaatct ggatataaga tcgttggtgt
     4381 cgatgtcagc tccggagttg agacaaatgg tgttcaggat ctcgataaga tacgttcatt
     4441 tgtccaagca gcaaagagtg ccttctagtg atttaatagc tccatgtcaa caagaataaa
     4501 acgcgtttcg ggtttacctc ttccagatac agctcatctg caatgcatta atgcattgga
     4561 cctcgcaacc ctagtacgcc cttcaggctc cggcgaagca gaagaatagc ttagcagagt
     4621 ctattttcat tttcgggaga cgagatcaag cagatcaacg gtcgtcaaga gtcctacgag
     4681 actgaggaat ccgctcttgg ctccacgcga ctatatattt gtctctaatt gtactttgac
     4741 atgctcctct tctttactct gatagcttga ctatgaaaat tccgtcacca gcccctgggt
     4801 tcgcaaagat aattgcactg tttcttcctt gaactctcaa gcctacagga cacacattca
     4861 tcgtaggtat aaacctcgaa aatcattcct actaagatgg gtatacaata gtaaccatgc
     4921 atggttgcct agtgaatgct ccgtaacacc caatacgccg gccgaaactt ttttacaact
     4981 ctcctatgag tcgtttaccc agaatgcaca ggtacacttg tttagaggta atccttcttt
     5041 ctagaagtcc tcgtgtactg tgtaagcgcc cactccacat ctccactcgc tccggggaga
     5101 cgggcacaag gttcatgaac cgaagaggat gggaaccccg accatggcct gcggccgcag
     5161 ctatcagtcc tctcagctga cgcccctcac gaaccctcgc actctcccga aatgctcttc
     5221 ggtgtccatg gcgcatcatc tcagcagcat cccatgtcaa accatggctt tgggcccacg
     5281 gactctgtgg ccctgccgca acatcaccat caccatcgac tcccgcccca tgcagcgctg
     5341 cgcgccccag ggcgtcatgg agtgaatgtg gaatctcctc ctttgccctc gggcccgccg
     5401 agtgtggtgg gccagcctgg aatgcctgat ccagccccca ggcctcgagg accaaaactg
     5461 aagtttactc ccgaagagga cgctctactg gtggagttga aggaaaacaa gaacttgacg
     5521 tggaagcaaa ttgcagactt cttcccgggc cgaacgagcg gtaccttgca agtccgatac
     5581 tgcaccaagc tgaaggctaa ggatgtagct tggagtgacg aaatggttcg atttgctcct
     5641 gatgtatttc tacgcctgtc tcacacatgc taatgaaggg aataggtaca aaggctgcag
     5701 cgggcaatgc acgagtacga gaacgatcgg tggcgcatca ttgcagggaa ggttggaaat
     5761 ggcttcaccc cagctgcttg ccgcgagaaa gccatgcagc tccatgagta aaagcgttgg
     5821 ggaattttca tatttatatc tactgtcgcc agattcggcc ctgcttggac cctctgatct
     5881 ccttactctc catattggtt caaatgtcgg gtcaccgata gggctggtgg tgcaggcttg
     5941 ttgtaggcac gggaggatga tcagcataac tctgagtcac tatagggacg ggttgatgta
     6001 aggtattaag tgatgtatga taattcattt tagcccgggg gaacatatgg cgccggcatt
     6061 tgttcgttcg caatgaaccg acactagcgt ccgctctcgc agtttagcac cggctgatcc
     6121 cgggctgaac gcggccattg ctcggccggg gcatgtgttc cttatctacg gcagaccgca
     6181 gatgaccact ggagcagatt atagacccta agccctaagc cggacaccca atcgagtagg
     6241 tctgcggacc aggtcactgc gggcagccgg agaagctccg caaccaatca atccccggcg
     6301 ctgactaagg gcaggcgacc acgggccgaa gcggcttcaa actcacctca acctccaaac
     6361 tccctcatct ccaaacgtcc ttgccttgtc tgccgtcatt gcaacccacc caccaggaca
     6421 catgagcagc aagtcccagc tcacctacac tgcccgtgcc agcaagcatc ccaatgctct
     6481 ggcgaagagg ctgttcgaga ttgccgaggc caagaaaacc aatgtgactg tctcggctga
     6541 cgttaccacc actaaggagc tactagatct tgctgaccgt ctcggtccct acattgccgt
     6601 gatcaaaacc cacatcgata tcctctctga tttcagcaac gagactattg agggacttaa
     6661 ggctctcgcg cagaagcaca actttctcat cttcgaggac cgcaagttca ttgacatcgg
     6721 caacacggtc cagaagcaat accacggcgg taccctccgt atctcggaat gggcccacat
     6781 catcaactgc agcattctcc ctggtgaggg tatcgtcgag gctctcgctc agacggcgtc
     6841 tgcaccggac ttcgcctacg gccccgaacg cggtctgttg atcttggcag agatgacctc
     6901 taagggctcc ttggctaccg gccagtacac tacttcctcg gtcgattatg cccggaaata
     6961 caagaacttc gttatgggat tcgtgtcgac gcgcgcgttg ggtgaggtgc agtcggaagt
     7021 cagctctcct tcggatgagg aggactttgt ggttttcacg actggtgtga acattaacct
     7081 ccaagtcgca attgacctac gcttatgctg cagacgcgtt acgtatcgga tccagaattc
     7141 gtgatatctg aattcgtcga caagcttatt ttttgtatac tgttttgtga tagcacgaag
     7201 tttttccacg gtatcttgtt aaaaatatat atttgtggcg ggcttaccta catcaaatta
     7261 ataagagact aattataaac taaacacaca agcaagctac tttagggtaa aagtttataa
     7321 atgcttttga cgtataaacg ttgcttgtat ttattattac aattaaaggt ggatagaaaa
     7381 cctagagact agttagaaac taatctcagg tttgcgttaa actaaatcag agcccgagag
     7441 gttaacagaa cctagaaggg gactagatat ccgggtaggg aaacaaaaaa aaaaaacaag
     7501 acagccacat attagggaga ctagttagaa gctagttcca ggactaggaa aataaaagac
     7561 aatgatacca cagtctagtt gacaactaga tagattctag attgaggcca aagtctctga
     7621 gatccaggtt agttgcaact aatactagtt agtatctagt ctcctataac tctgaagcta
     7681 gaataactta ctactattat cctcaccact gttcagctgc gcaaacggag tgattgcaag
     7741 gtgttcagag actagttatt gactagtcag tgactagcaa taactaacaa ggtattaacc
     7801 taccatgtct gccatcaccc tgcacttcct cgggctcagc agccttttcc tcctcatttt
     7861 catgctcatt ttccttgttt aagactgtga ctagtcaaag actagtccag aaccacaaag
     7921 gagaaatgtc ttaccacttt cttcattgct tgtctctttt gcattatcca tgtctgcaac
     7981 tagttagagt ctagttagtg actagtccga cgaggacttg cttgtctccg gattgttgga
     8041 ggaactctcc agggcctcaa gatccacaac agagccttct agatgactgg tcaataacta
     8101 gttggtcttt gtctgagtct gacttacgag gttgcatact cgctcccttt gcctcgtcaa
     8161 tcgatgagaa aaagcgccaa aactcgcaat atggctttga accacacggt gctgagacta
     8221 gttagaatct agtcccaaac tagcttggat agcttacctt tgccctttgc gttgcgacag
     8281 gtcttgcagg gtatggttcc tttctcacca gctgatttag ctgccttgct accctcacgg
     8341 cggatctgca taaagagtgg ctagaggtta taaattagca ctgatcctag gtacggggct
     8401 gaatgtaact tgcctttcct ttctcatcgc gcggcaagac aggcttgctc aaattcctac
     8461 cagtcacagg ggtatgcacg gcgtacggac cacttgaact agtcacagat tagttagcaa
     8521 ctagtctgca ttgaatggct gtacttacgg gccctcgcca ttgtcctgat catttccagc
     8581 ttcaccctcg ttgctgcaaa gtagttagtg actagtcaag gactagttga aatgggagaa
     8641 gaaactcacg aattctcgac acccttagta ttgtggtcct tggacttggt gctgctatat
     8701 attagctaat acactagtta gactcacaga aacttacgca gctcgcttgc gcttcttggt
     8761 aggagtcggg gttgggagaa cagtgccttc aaacaagcct tcataccatg ctacttgact
     8821 agtcagggac tagtcaccaa gtaatctaga taggacttgc ctttggcctc catcagttcc
     8881 ttcatagtgg gaggtccatt gtgcaatgta aactccatgc cgtgggagtt cttgtccttc
     8941 aagtgcttga ccaatatgtt tctgttggca gagggaacct gtcaactagt taataactag
     9001 tcagaaacta gtatagcagt agactcactg tacgcttgag gcatcccttc actcggcagt
     9061 agacttcata tggatggata tcaggcacgc cattgtcgtc ctgtggacta gtcagtaact
     9121 aggcttaaag ctagtcgggt cggcttacta tcttgaaatc cggcagcgta agctccccgt
     9181 ccttaactgc ctcgagatag tgacagtact ctggggactt tcggagatcg ttatcgcgaa
     9241 tgctcggcat actaatcgtt gactagtctt ggactagtcc cgagcaaaaa ggattggagg
     9301 aggaggagga aggtgagagt gagacaaaga gcgaaataag agcttcaaag gctatctcta
     9361 agcagtatga aggttaagta tctagttctt gactagattt aaaagagatt tcgactagtt
     9421 atgtacctgg agtttggata taggaatgtg ttgtggtaac gaaatgtaag ggggaggaaa
     9481 gaaaaagtcg gtcaagaggt aactctaagt cggccattcc tttttgggag gcgctaacca
     9541 taaacggcat ggtcgactta gagttagctc agggaattta gggagttatc tgcgaccacc
     9601 gaggaacggc ggaatgccaa agaatcccga tggagctcta gctggcggtt gacaacccca
     9661 ccttttggcg tttctgcggc gttgcaggcg ggactggata cttcgtagaa ccagaaaggc
     9721 aaggcagaac gcgctcagca agagtgttgg aagtgatagc atgatgtgcc ttgttaacta
     9781 ggtcaaaatc tgcagtatgc ttgatgttat ccaaagtgtg agagaggaag gtccaaacat
     9841 acacgattgg gagagggcct aggtataaga gtttttgagt agaacgcatg tgagcccagc
     9901 catctcgagg agattaaaca cgggccggca tttgatggct atgttagtac cccaatggaa
     9961 agcctgagag tccagtggcg ctaattcatg aataacggtg agactagcgg ccggtcccct
    10021 tatcccagct gttccacgtt ggcctgcccc tcagttagcg ctcaactcaa tgcccctcac
    10081 tggcgaggcg agggcaagga tggaggggca gcatcgcctg agttggagca aagcggccgc
    10141 catgggagca gcgaaccaac ggagggatgc cgtgctttgt cgtggctgct gtggccaatc
    10201 cgggcccttg gttggctcac agagcgttgc tgtgagtcca tgagctatta ttgctaggta
    10261 cagtatagag agaggagaga gagagagaga gagagagggg aaaaaaggtg aggttgaagt
    10321 gagaaaaaaa aaaaaaaaaa tccaaccact gacggctgcc ggctctgcca cccccctccc
    10381 tccaccccag accacctgca cactcagcgc gcagcatcac ctaatcttgg ctcgccttcc
    10441 cgcagctcag gttgtttttt ttttctctct ccctcgtcga agccgccctt gttcccttat
    10501 ttatttccct ctccatcctt gtctgccttt ggtccatctg cccctttgtc tgcatctctt
    10561 ttgcacgcat cgccttatcg tcgtctcttt tttcactcac gggagcttga cgatgacctg
    10621 actcgtgagc ctcacctgct gatttctctc cccccctccc gaccggcttg acttttgttt
    10681 ctcctccagt accttatcgc gaagccggaa gaacctctta acctctagat gaaaaagcct
    10741 gaactcaccg cgacgtctgt cgagaagttc ctgatcgaaa agttcgacag cgtctccgac
    10801 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
    10861 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
    10921 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
    10981 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
    11041 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
    11101 gccgatctca gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
    11161 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
    11221 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
    11281 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
    11341 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
    11401 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
    11461 cagacgcgct acttcgagcg gaggcacccg gagcttgcag gatcgccgcg gctccgggcg
    11521 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
    11581 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
    11641 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
    11701 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagatgcat
    11761 ggctttcgtg accgggcttc aaacaatgat gtgcgatggt gtggttcccg gttggcggag
    11821 tctttgtcta ctttggttgt ctgtcgcagg tcggtagacc gcaaatgagc aactgatgga
    11881 ttgttgccag cgatactata attcacatgg atggtctttg tcgatcagta gctagtgaga
    11941 gagagagaac atctatccac aatgtcgagt gtctattaga catactccga gaataaagtc
    12001 aactgtgtct gtgatctaaa gatcgattcg gcagtcgagt agcgtataac aactccgagt
    12061 accagcgaaa gcacgtcgtg acaggagcag ggctttgcca actgcgcaac cttgcttgaa
    12121 tgaggataca cggggtgcaa catggctgta ctgatccatc gcaaccaaaa tttctgttta
    12181 tagatcaagc tggtagattc caattactcc acctcttgcg cttctccatg acatgtaagt
    12241 gcacgtggaa accataccca aattgcctac agctgcggag catgagccta tggcgatcag
    12301 tctggtcatg ttaaccagcc tgtgctctga cgttaatgca gaatagcttc gcgtgatctt
    12361 acggcattat acgtatgatc ggtccacgat cagctagatt atctagtcag cttgatgtca
    12421 tagctgtttc ctgaggctca atactgacca tttaaatcat acctgacctc catagcagaa
    12481 agtcaaaagc ctccgaccgg aggcttttga cttgatcggc acgtaagagg ttccaacttt
    12541 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
    12601 gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt cttgcttgaa gccgcgatta
    12661 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
    12721 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
    12781 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcaggct aaactggctg
    12841 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
    12901 ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat
    12961 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
    13021 gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
    13081 atgaataacg gtttggttgg tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
    13141 gaacaagtct ggaaagaaat gcataaactc ttgccattct caccggattc agtcgtcact
    13201 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
    13261 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
    13321 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
    13381 cctgatatga ataaattgca gtttcacttg atgctcgatg agtttttcta atgaggacct
    13441 aaatgtaatc acctggctca ccttcgggtg ggcctttctg cgttgctggc gtttttccat
    13501 aggctccgcc cccctgacga gcatcacaaa aatcgatgct caagtcagag gtggcgaaac
    13561 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    13621 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    13681 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    13741 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    13801 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    13861 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    13921 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt tacctcggaa
    13981 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    14041 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgattttct
    14101 accgaagaaa ggcccacccg tgtaaaacga cggccagttt atctagtcag cttgattcta
    14161 gctgatcgtg gaccgga
//
